• Login
    View Item 
    •   Home
    • Colleges & Programs
    • Medical College of Georgia (MCG)
    • Department of Cellular Biology and Anatomy
    • Department of Cellular Biology and Anatomy: Faculty Research and Presentations
    • View Item
    •   Home
    • Colleges & Programs
    • Medical College of Georgia (MCG)
    • Department of Cellular Biology and Anatomy
    • Department of Cellular Biology and Anatomy: Faculty Research and Presentations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of Scholarly CommonsCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsThis CollectionTitleAuthorsIssue DateSubmit DateSubjects

    My Account

    LoginRegister

    About

    AboutCreative CommonsAugusta University LibrariesUSG Copyright Policy

    Statistics

    Display statistics

    Reduced-folate carrier (RFC) is expressed in placenta and yolk sac, as well as in cells of the developing forebrain, hindbrain, neural tube, craniofacial region, eye, limb buds and heart.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    1471-213X-3-6.pdf
    Size:
    619.4Kb
    Format:
    PDF
    Download
    Authors
    Maddox, Dennis M
    Manlapat, Anna K
    Roon, Penny
    Prasad, Puttur D
    Ganapathy, Vadivel
    Smith, Sylvia B
    Issue Date
    2003-10-29
    URI
    http://hdl.handle.net/10675.2/98
    
    Metadata
    Show full item record
    Abstract
    BACKGROUND: Folate is essential for cellular proliferation and tissue regeneration. As mammalian cells cannot synthesize folates de novo, tightly regulated cellular uptake processes have evolved to sustain sufficient levels of intracellular tetrahydrofolate cofactors to support biosynthesis of purines, pyrimidines, and some amino acids (serine, methionine). Though reduced-folate carrier (RFC) is one of the major proteins mediating folate transport, knowledge of the developmental expression of RFC is lacking. We utilized in situ hybridization and immunolocalization to determine the developmental distribution of RFC message and protein, respectively. RESULTS: In the mouse, RFC transcripts and protein are expressed in the E10.0 placenta and yolk sac. In the E9.0 to E11.5 mouse embryo RFC is widely detectable, with intense signal localized to cell populations in the neural tube, craniofacial region, limb buds and heart. During early development, RFC is expressed throughout the eye, but by E12.5, RFC protein becomes localized to the retinal pigment epithelium (RPE). CONCLUSIONS: Clinical studies show a statistical decrease in the number of neural tube defects, craniofacial abnormalities, cardiovascular defects and limb abnormalities detected in offspring of female patients given supplementary folate during pregnancy. The mechanism, however, by which folate supplementation ameliorates the occurrence of developmental defects is unclear. The present work demonstrates that RFC is present in placenta and yolk sac and provides the first evidence that it is expressed in the neural tube, craniofacial region, limb buds and heart during organogenesis. These findings suggest that rapidly dividing cells in the developing neural tube, craniofacial region, limb buds and heart may be particularly susceptible to folate deficiency.
    Citation
    BMC Dev Biol. 2003 Jul 29; 3:6
    ae974a485f413a2113503eed53cd6c53
    10.1186/1471-213X-3-6
    Scopus Count
    Collections
    Department of Cellular Biology and Anatomy: Faculty Research and Presentations

    entitlement

    Related articles

    • Spatial and temporal expression of folate-binding protein 1 (Fbp1) is closely associated with anterior neural tube closure in mice.
    • Authors: Saitsu H, Ishibashi M, Nakano H, Shiota K
    • Issue date: 2003 Jan
    • Expression of folate receptor alpha in the mammalian retinol pigmented epithelium and retina.
    • Authors: Smith SB, Kekuda R, Gu X, Chancy C, Conway SJ, Ganapathy V
    • Issue date: 1999 Apr
    • SOX13 exhibits a distinct spatial and temporal expression pattern during chondrogenesis, neurogenesis, and limb development.
    • Authors: Wang Y, Ristevski S, Harley VR
    • Issue date: 2006 Dec
    • [Advances in the study of the etiologic relationship between reduced folate carrier gene (RFC1) and neural tube and craniofacial defects].
    • Authors: Pei LJ, Li Z
    • Issue date: 2004 Mar
    • A humanized mouse model for the reduced folate carrier.
    • Authors: Patterson D, Graham C, Cherian C, Matherly LH
    • Issue date: 2008 Feb
    DSpace software (copyright © 2002 - 2021)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.