Heat shock response in CHO mammalian cells is controlled by a nonlinear stochastic process.
MetadataShow full item record
AbstractIn many biological systems, the interactions that describe the coupling between different units in a genetic network are nonlinear and stochastic. We study the interplay between stochasticity and nonlinearity using the responses of Chinese hamster ovary (CHO) mammalian cells to different temperature shocks. The experimental data show that the mean value response of a cell population can be described by a mathematical expression (empirical law) which is valid for a large range of heat shock conditions. A nonlinear stochastic theoretical model was developed that explains the empirical law for the mean response. Moreover, the theoretical model predicts a specific biological probability distribution of responses for a cell population. The prediction was experimentally confirmed by measurements at the single-cell level. The computational approach can be used to study other nonlinear stochastic biological phenomena.
CitationPLoS Comput Biol. 2007 Oct 5; 3(10):e187
- Sensing the heat stress by Mammalian cells.
- Authors: Cates J, Graham GC, Omattage N, Pavesich E, Setliff I, Shaw J, Smith CL, Lipan O
- Issue date: 2011 Aug 11
- Stochastic modelling of the eukaryotic heat shock response.
- Authors: Mizera A, Gambin B
- Issue date: 2010 Aug 7
- Mathematical modeling of heat shock protein synthesis in response to temperature change.
- Authors: Szymańska Z, Zylicz M
- Issue date: 2009 Aug 7
- Stochastic effects in a model of nematode infection in ruminants.
- Authors: Marion G, Renshaw E, Gibson G
- Issue date: 1998 Jun
- Mild heat induces a distinct "eustress" response in Chinese Hamster Ovary cells but does not induce heat shock protein synthesis.
- Authors: Peksel B, Gombos I, Péter M, Vigh L Jr, Tiszlavicz Á, Brameshuber M, Balogh G, Schütz GJ, Horváth I, Vigh L, Török Z
- Issue date: 2017 Nov 15