• Characterization of the transcriptome profiles related to globin gene switching during in vitro erythroid maturation

      Li, Biaoru; Ding, Lianghao; Li, Wei; Story, Michael D; Pace, Betty S.; Department of Pediatrics (2012-04-26)
      Background: The fetal and adult globin genes in the human b-globin cluster on chromosome 11 are sequentially expressed to achieve normal hemoglobin switching during human development. The pharmacological induction of fetal g-globin (HBG) to replace abnormal adult sickle bS-globin is a successful strategy to treat sickle cell disease; however the molecular mechanism of g-gene silencing after birth is not fully understood. Therefore, we performed global gene expression profiling using primary erythroid progenitors grown from human peripheral blood mononuclear cells to characterize gene expression patterns during the g-globin to b-globin (g/b) switch observed throughout in vitro erythroid differentiation