• To seal or To Not Seal

      Jefferson, Tiffany; Verdree, Wakia; Dental Hygiene (2020-02)
      This project sought to determine why sealants are considered the best preventative method to prevent caries in permanent molars.
    • The Future of Nano-Robots in Dentistry

      Cartledge, Brenna; Watson, Lindsey; Dental Hygiene (2020-02)
      Micro-robots are being used in the medical field for a variety of procedures, such as: drug delivery and precision therapeutics. This research is used to determine how micro-robots can be incorporated into dentistry.
    • How Effective is Velscope in Dentistry

      Abbkar, Fatima; Atchison, Sherina; Dental Hygiene (2020-02)
      Velscope is a handheld device that used to detect oral cancer as an adjunct with the intraoral physical examination, using autofluorescence. The purpose of this article is to evaluate the effectiveness of velscope in dentistry.
    • Scaling and Root Planing in Conjunction with Propolis Irrigation

      Kurowski, Ashley; Lowe, Elaine; Dental Hygiene (2020-02)
      Periodontal Disease is a highly prevalent oral disease in the United States and dental professionals are always seeking a new and improved method to help decrease or arrest the side effects. This research is looking to propose a new innate, financially feasible option for individuals battling periodontal disease.
    • The Effects of Erythritol on Dental Caries and Biofilm

      Carter, Shelby; Gailer, Hannah; Dental Hygiene (2020-02)
      This project sought to determine the effectiveness of erythritol on the prevention of dental caries and the reduction of dental biofilm.
    • A Dental Hygienist’s Role in Teledentistry

      Harmon, SaDora; Barajas, Tania; Dental Hygiene (2020-02)
      To educate Teledentistry as the new advancement in Dentistry. To elaborate on the Dental hygienist’s role in this new advancement.
    • The Effects Probiotics Have on The Oral Cavity

      Colbert, Antonisha; Hamm, Marissa; Dental Hygiene (2020-02)
      The purpose of this literature review is to explore the options of probiotics; to see if they could potentially inhibit the bad bacteria in the oral cavity.
    • Utilizing Salivary Diagnostics to Prevent Oral Disease

      Delagarza Siquian, Kristen; Giacobone, Madison; Dental Hygiene (2020-02)
      Evaluation of xerostomia should be done at every appointment to assess the patient’s comfort level and implement a preventative care plan by recommending safe and effective products for each patient. By performing salivary diagnostics and using a clinical oral dryness score (CODS), dental hygienists are able to best assess a patient’s needs in order to prevent dental caries, halitosis, periodontal disease, and opportunistic infections.2 By providing an individualized care plan based on each patients’ CODS, dental hygienists can have a positive impact on their patients’ overall oral health.
    • Whitening Alternatives for Patients with Enamel Defects

      Kinsler, Abby; Smeragliuolo, Jenny; Dental Hygiene (2020-02)
      The purpose of our literature review is to explore the alternative whitening options available to patients who suffer from enamel defects. For moderate to severe cases, most treatment plans include invasive restorations to cover the enamel surface, which may result in tooth sensitivity and a high cost. The benefits of exploring these new techniques offer treatment options to patients that may be less expensive, less invasive, and a timelier treatment time which would result in an improved esthetic appearance faster
    • Oral Manifestations of Crohn’s Disease and its effect on Dental Treatment

      Rudduck, Alannah; Watkins, Meghan; Dental Hygiene (2020-02)
      OBJECTIVES: The purpose of this literature review is to inform dental health care professionals about CD, the oral manifestations that may be present, and what treatment modifications may be necessary. METHODS: To research information for this paper, we using the keywords: “Crohn’s Disease,” “oral manifestations,” and “dental treatment.” We gathered the majority of our articles through PubMed and EBSCO. We narrowed our search criteria by eliminating sources that were greater than five years old and articles that were not peer-reviewed. RESULTS: In order to understand CD, there is a lot that still remains unknown, and much more research needs to be conducted. However, understanding the biomarker in recent studies is the most important factor in any health care profession. The use of interprofessional communication with the patient's nutritionist, physician, and dental health team to determine key factors that are associated with CD is currently very important for further research. It’s theorized that IL12 cytokine stimulation in Th1 mediated upregulation results of IFNY may be the primary factors of CD.4 The reasons why it is significant to be conscious of the signs and symptoms of CD is due to oral inflammation may precede the intestinal manifestations.4 In addition, it’s hypothesized that the inflammatory response of IBD raises the basal cytokine response that induces periodontal disease. Several studies have been looking into this relationship further. Additional studies have recognized that CD patients have higher attribution of periodontal disease, deeper pocket depth, and clinical attachment loss.4 CONCLUSIONS: Treatment alterations to make the patient more comfortable include scheduling short appointments in the morning and allowing time for frequent restroom breaks. Despite having minimal biofilm accumulation, these patients tend to have bleeding on probing, deep periodontal pockets, decay, missing teeth and/or extensive previous dental treatment.3 Thus, clinicians need to regularly monitor caries and periodontal risks to optimize CD patient’s oral health status. Knowledgeable clinicians that recognize CD signs and symptoms should refer patients to their physicians for further diagnosis. It’s believed the more that is learned about CD and other IBD, the more overlap we will see in the oral cavity. Limitations to our research include inconsistent data and misrepresented populations. To best benefit the population, research will need to continue in order to learn more about the disease, development and management, and ways to prevent CD and other IBD, as well as less aggressive treatment options.
    • The Clinical Aspects of Hypophosphatasia

      Baker, Abby; Simpson, Sage; Dental Hygiene (2020-02-13)
      OBJECTIVES: The purpose of this research is to educate the dental community on the effects of hypophosphatasia and the clinical manifestations it presents throughout the body and the oral cavity. METHODS: Hypophosphatasia is a rare inherited metabolic disorder that has a wide spectrum of disease presentation. Due to 300 types of ALP mutations, HPP has six different major forms: perinatal lethal, prenatal benign, infantile, childhood, adulthood, and odotohypophosphatasia. There are clinical signs presented throughout the body and the oral cavity. Premature exfoliation of primary dentition is the first clinical sign of hypophosphatasia in childhood. Two different studies were reviewed in order to compare similar symptoms of hypophosphatasia. RESULTS: In one case study there were 38 patients who reported similar symptoms. In 15 (39%) of the patients a history of fractures was present. In the same study 21 (55%) of the patients had recurring headaches, 4 (11%) of the patients experienced severe muscle weakness, 23 (61%) experienced recurring muscle pain, and 18 (47%) of the patients exhibited dental abnormalities. In another case study there were 9 patients that reported only dental signs of hypophosphatasia. Dental signs of HPP were shown in childhood in 8 (88%) of the patients. The premature loss of the primary dentition was shown in 7 (77%) of the patients, absent primary dentition in 1 (11%) of the patients, and delayed loss of primary teeth in 1 (11%) of the patients. CONCLUSIONS: Since hypophosphatasia is so rare, more studies are needed on the diagnosis, preventative methods, and treatments. The majority of HPP cases are diagnosed in adulthood which ensures the disorder could have been present during childhood and was overlooked. In order to diagnose the disorder promptly, there should be a thorough medical history and clinical signs must be evaluated. There is no cure or long term prognosis for the hypophosphatasia disorder.
    • Dendritic Cell Derived Exosomes Loaded with Immunoregulatory Cargo Reprogram local Immune Responses and Inhibit Degenerative Bone Disease In vivo

      Elashiry, Mahmoud; Biomedical Sciences (Augusta University, 2020-12)
      Background: Histopathological study of periodontitis (PD) lesions at sites of bone loss reveals infiltration with dendritic cells (DC) CD4+ T cell clusters and other inflammatory cells. DCs can direct bone protective T-regulatory cell (Tregs) responses, or bone destructive T-helper 17 (Th17). The use of exosomes (EXO), natural nanoparticles released by DCs and other cells, are under intense scrutiny in clinical trials for autoimmune diseases and cancer, but no studies to date have harnessed DC-derived EXO to regulate alveolar bone loss in PD. Aim: To determine the ability of custom DC-derived EXO to reprogram immune cell functions of recipient DCs and T cells and mitigate inflammatory bone loss in mice. Methods: Murine bone marrow derived donor DC subtypes, including immune regulatory DCs (regDC), immature DCs (iDC) and immune stimulatory (stimDC) DCs were the source of purified DC EXO. Reg DC EXO were actively loaded with TGFB1/IL10 using ultrasonication. Preliminary in vitro studies of EXO cargo, stability and resistance of cytokine cargo to proteolysis, as well as immune functions and osteoclastogenesis was investigated. The following DC EXO subtypes were then tested in vivo in six groups of mice, in the ligature induced PD model: Group 1, no ligature, Groups 2, 3, 4, 5 and 6, 8 ligature plus gingival injection of, respectively, PBS, regDC EXO, iDC EXO, stimDC EXO and free TGFB1/IL10. Biodistribution and in vivo uptake of EXO by gingival recipient DCs and T cells were tracked. The ability of DC EXO to modulate gingival recipient DC and CD4 T cells and cytokine expression was confirmed. TRAP staining of histological sections measured osteoclast number, while bone loss volume was measured in 3-D by micro-CT. Results: Injected EXO showed a high affinity for gingival site of inflammatory bone loss. RegDC EXO containing TGFb/IL-10 cargo, protected cargo against proteolytic degradation and were taken up by recipient DCs and T cells in vivo, promoting Tregs, while inhibiting Th17 recruitment and inhibiting bone loss. In contrast, EXO subtypes lacking TGFb/IL-10 or free TGFB/IL-10 did not shift the Treg-Th17 balance and did not inhibit bone loss. Mechanistically, a key role for TGFb1 in induction of Tregs by regDC EXO was found using blocking antibodies to TGFb and/or IL-10. T.E.M. analysis revealed TGFb1 localized in the EXO lumen and in the transmembrane domain, which sustained signaling in recipient DCs. Blocking experiments revealed that sustainable prolonged TGFb1 signaling required initial interaction between regDCs EXO and TGFBR1 complex on acceptor cells, followed by internalization of regDC EXO with TGFB1-TGFBR1 complex for sustained SMAD2/3 phosphorylation. Conclusion: This is the first study to demonstrate the efficacy of DCs exosomes for inhibition of experimental bone loss and the cellular immune mechanisms involved. This provides the basis for a future novel immunotherapeutic strategy for PD in humans.
    • The effects of altered salivary viscosity on the incidence and extent of dental caries in rats

      Biesbrock, Aaron Reed; Department of Oral Pathology (Augusta University, 1989-05-02)
      The research was intended to determine whether alterations in salivary viscosity brought about by altering dietary components affected the incidence and extent of dental caries in rats.
    • The stability of an increased vertical dimension of occlusion in growing and non-growing golden hamsters

      Berman, Scott C.; Department of Oral Biology (Augusta University, 1993-03)
    • Toxicity of visible light-cured denture resins

      Barron, Dara Jewell; Department of Oral Biology (1992-04)
      In this study three commercial formulations of visible light-cured (VLC) denture resins have been analyzed. The products used are those suggested for the reline, repair and fabrication of dentures to improve their fit. The biocompatibility of these resins was investigated by measuring RNA and DNA synthesis of oral epithelial cells in vitro. The extent to which oral cells recover from toxic resin exposure, the conversion of monomer into polymer, the presence of inorganic filler, and resin leacha~ility have also been studied. It was shown that VLC denture resins inhibit the synthesis of RNA and· DNA relative to a heat-cured resin control (p~0.05). Although epithelial cells appeared to recover from toxic resin exposure, this recovery was inconsistent among experiments. Infrared spectroscopy illustrated chemical group differences that occurred before and after photo-polymerization. Using these differences, the conversion of monomer into polymer ranged from 77% to 97%. This conversion was significantly affected (p< .003) by the type of curing unit, duration of photo-polymerization, and surface exposed to visible light. Soluble substances in cured and uncured resin products were analogous using HPLC. The range of inorganic filler present was 0-15%. These investigations suggest that visible light-cured denture resins may impair the replication of oral epithelial cells. This effect may be related to the leachability of unpolymerized resin constituents, the presence or absence of filler particles, or polymerization by-products.
    • Efficacy of Epigallocatechin-3-gallate-palmitate as a Virucidal Compound Against Norovirus

      Widjaja, Nicole; Department of Oral Biology and Diagnostic Sciences (Augusta University, 2020-05)
      Norovirus is a highly infectious, non-enveloped virus found to be the leading cause of global gastroenteritis outbreaks. Every year within the United States, this virus is responsible for an average of 19-21 million cases of acute gastroenteritis, approximately 570-800 deaths, and has been the cause of 1.7 to 1.9 million outpatient visits. On a global scale, healthcare costs and lost productivity are estimated to $60 billion due to illnesses and outbreaks caused by the burden of norovirus. Unfortunately, current measures to prevent the transmission of norovirus remain insufficient as the Center for Disease Control and Prevention (CDC) can only recommend hand washing with soap and water as the best preventative measure. The only other hand hygiene method available is alcohol-based hand sanitizers, but the CDC states that they are not effective in inactivating norovirus particles and warns that it should not be considered a substitute to hand washing. Recently, epigallocatecin-3-gallate (EGCG) a major component extracted from the leaves of Camellia sinensis, also commonly known as tea plant, has shown potential to be the next viable candidate as an antiviral solution. Lipid derivatives of EGCG, most notably EGCGpalmitate, has shown to express potent antiviral properties and has showed to play a crucial role in the fight against other non-enveloped viruses such as poliovirus and adenovirus. In this study, we determined the efficacy of EGCG-palmitate in novel formulations against human norovirus surrogates by utilizing the EU international standards for hand hygiene in vitro studies against norovirus. Evidence is provided determining the virucidal activity of alcohol-based ProtecTeaV formulations containing EGCG-palmitate as well as the potential for EGCG-palmitate as a persistent residual virucidal activity against norovirus surrogates, feline calicivirus (FCV) and murine norovirus-1 (MNV-1). By creating an effective, environmentally friendly, non-toxic and long lasting solution composed of EGCG-palmitate, the results of this innovative approach would expand the options available to reduce the transmission of norovirus essentially bridging the gap for a new preventative hand hygiene and ultimately impacting the spread of norovirus on a worldwide scale.
    • The Application of Low-Cost, Close-Range Photogrammetry in Dentistry

      Patel, Mohit; Mettenburg, D.; Biological Sciences, Restorative Sciences (Augusta University Libraries, 2020-05-05)
      This item presents the abstract for a poster presentation at the 21st Annual Phi Kappa Phi Student Research and Fine Arts Conference.
    • Influence of Porphyromonas gingivalis on Anti-Apoptotic/Autophagic Signaling Pathways in Human Dendritic Cells

      Meghil, Mohamed; Tawfik, Omnia; Elashirty, Mahmoud; Rajendran, Mythilypriya; Arce, Roger; Schoenlein, Patricia V.; Cutler, Christopher; Department of Oral Biology & Diagnostic Sciences, Department of Periodontics, Department of Cellular Biology and Anatomy (Augusta University, 2019)
      The purpose of this study was to investigate the molecular mechanisims of P. gingivalis-mediated disruption of homeostatic apoptosis and autophagy in DCs.
    • Murine Dendritic Cell Interactions with Minor-fimbriae P. gingivalis

      Yuan, J; Auersvald, c; Elashiry, M; Meghil, M; Elashiry, M; Finger Stadler, A; Arce, R.M.; Department of Periodontics (Augusta University, 2019)
      The objective was to determine DC maturity phenotype of murine bone marrow-derived DCs in response to wild/type (PgWT) and minor-fimbriated DPG3.