Cortical gene transcription response patterns to water maze training in aged mice
Stranahan, Alexis M.
Becker, Kevin G.
MetadataShow full item record
AbstractBackground: The hippocampus mediates the acquisition of spatial memory, but the memory trace is eventually transferred to the cortex. We have investigated transcriptional activation of pathways related to cognitive function in the cortex of the aged mouse by analyzing gene expression following water maze training.
Results: We identified genes that were differentially responsive in aged mice with accurate spatial performance during probe trials or repeated swimming sessions, relative to home cage conditions. Effective learners exhibited significantly greater activation of several pathways, such as the mitogen-activated protein kinase and insulin receptor signaling pathways, relative to swimmers. The genes encoding activity-related cytoskeletal protein (Arc) and brain-derived neurotrophic factor (BDNF) were upregulated in proficient learners, relative to swimmers and home cage controls, while the gene encoding Rho GTPase activating protein 32 (GRIT) was downregulated. We explored the regulation of Arc, BDNF, and GRIT expression in greater morphological detail using in situ hybridization. Recall during probe trials enhanced Arc expression across multiple cortical regions involved in the cognitive component of water maze learning, while BDNF expression was more homogeneously upregulated across cortical regions involved in the associational and sensorimotor aspects of water maze training. In contrast, levels of GRIT expression were uniformly reduced across all cortical regions examined.
Conclusions: These results suggest that cortical gene transcription is responsive to learning in aged mice that exhibit behavioral proficiency, and support a distributed hypothesis of memory storage across multiple cortical compartments.
CitationBMC Neurosci. 2011 Jun 29; 12:63
- Reduced cortical BDNF expression and aberrant memory in Carf knock-out mice.
- Authors: McDowell KA, Hutchinson AN, Wong-Goodrich SJ, Presby MM, Su D, Rodriguiz RM, Law KC, Williams CL, Wetsel WC, West AE
- Issue date: 2010 Jun 2
- Hippocampal BDNF mediates the efficacy of exercise on synaptic plasticity and cognition.
- Authors: Vaynman S, Ying Z, Gomez-Pinilla F
- Issue date: 2004 Nov
- Spatial memory training modifies the expression of brain-derived neurotrophic factor tyrosine kinase receptors in young and aged rats.
- Authors: Silhol M, Arancibia S, Maurice T, Tapia-Arancibia L
- Issue date: 2007 May 25
- Prodynorphin knockout mice demonstrate diminished age-associated impairment in spatial water maze performance.
- Authors: Nguyen XV, Masse J, Kumar A, Vijitruth R, Kulik C, Liu M, Choi DY, Foster TC, Usynin I, Bakalkin G, Bing G
- Issue date: 2005 Jun 20
- Spatial learning and expression patterns of PP1 mRNA in mouse hippocampus.
- Authors: Haege S, Galetzka D, Zechner U, Haaf T, Gamerdinger M, Behl C, Hiemke C, Schmitt U
- Issue date: 2010