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dc.contributor.authorAl-Shabrawey, Mohamed
dc.contributor.authorAhmad, Saif
dc.contributor.authorMegyerdi, Sylvia
dc.contributor.authorOthman, Amira
dc.contributor.authorBaban, Babak
dc.contributor.authorPalenski, Tammy L.
dc.contributor.authorShin, Eui Seok
dc.contributor.authorGurel, Zafer
dc.contributor.authorHsu, Stephen
dc.contributor.authorSheibani, Nader
dc.date.accessioned2012-10-26T16:40:54Z
dc.date.available2012-10-26T16:40:54Z
dc.date.issued2012-07-14en_US
dc.identifier.citationMol Vis. 2012 Jul 14; 18:1895-1906en_US
dc.identifier.issn1090-0535en_US
dc.identifier.pmid22876114en_US
dc.identifier.urihttp://hdl.handle.net/10675.2/724
dc.description.abstractPurpose: The purpose of this study was to evaluate caspase-14 expression in the retina under normal and diabetic conditions, and to determine whether caspase-14 contributes to retinal microvascular cell death under high glucose conditions.
dc.description.abstractMethods: Quantitative real-time polymerase chain reaction and western blot analysis were used to evaluate caspase-14 expression in retinal cells, including pericytes (PCs), endothelial cells (ECs), astrocytes (ACs), choroidal ECs, and retinal pigment epithelium (RPE) cells. We also determined caspase-14 expression in the retinas of human subjects with or without diabetic retinopathy (DR) and in experimental diabetic mice. Retinal ECs and PCs were infected with adenoviruses expressing human caspase-14 or green fluorescent protein. Caspase-14 expression was also assessed in retinal vascular cells cultured under high glucose conditions. The number of apoptotic cells was determined with terminal deoxynucleotidyl transferase dUTP nick end labeling staining and confirmed by determining the levels of cleaved poly (ADP-ribose) polymerase-1 and caspase-3.
dc.description.abstractResults: Our experiments demonstrated that retinal ECs, PCs, ACs, choroidal ECs, and RPE cells expressed caspase-14, and DR was associated with upregulation and/or activation of caspase-14 particularly in retinal vasculature. High glucose induced marked elevation of the caspase-14 level in retinal vascular cells. There was a significant increase in the apoptosis rate and the levels of cleaved poly (ADP-ribose) polymerase-1 and caspase-3 in retinal ECs and PCs overexpressing caspase-14.
dc.description.abstractConclusions: Our findings indicate that caspase-14 might play a significant role in the pathogenesis of DR by accelerating retinal PC and EC death. Further investigations are required to elaborate the underlying mechanisms.
dc.rightsCopyright © 2012 Molecular Vision.en_US
dc.subjectResearch Articleen_US
dc.titleCaspase-14: A novel caspase in the retina with a potential role in diabetic retinopathyen_US
dc.typeArticleen_US
dc.identifier.pmcidPMC3413417en_US
dc.contributor.corporatenameDepartment of Oral Biology
dc.contributor.corporatenameDepartment of Ophthalmology
dc.contributor.corporatenameVision Discovery Institute
refterms.dateFOA2019-04-10T00:40:32Z
html.description.abstractPurpose: The purpose of this study was to evaluate caspase-14 expression in the retina under normal and diabetic conditions, and to determine whether caspase-14 contributes to retinal microvascular cell death under high glucose conditions.
html.description.abstractMethods: Quantitative real-time polymerase chain reaction and western blot analysis were used to evaluate caspase-14 expression in retinal cells, including pericytes (PCs), endothelial cells (ECs), astrocytes (ACs), choroidal ECs, and retinal pigment epithelium (RPE) cells. We also determined caspase-14 expression in the retinas of human subjects with or without diabetic retinopathy (DR) and in experimental diabetic mice. Retinal ECs and PCs were infected with adenoviruses expressing human caspase-14 or green fluorescent protein. Caspase-14 expression was also assessed in retinal vascular cells cultured under high glucose conditions. The number of apoptotic cells was determined with terminal deoxynucleotidyl transferase dUTP nick end labeling staining and confirmed by determining the levels of cleaved poly (ADP-ribose) polymerase-1 and caspase-3.
html.description.abstractResults: Our experiments demonstrated that retinal ECs, PCs, ACs, choroidal ECs, and RPE cells expressed caspase-14, and DR was associated with upregulation and/or activation of caspase-14 particularly in retinal vasculature. High glucose induced marked elevation of the caspase-14 level in retinal vascular cells. There was a significant increase in the apoptosis rate and the levels of cleaved poly (ADP-ribose) polymerase-1 and caspase-3 in retinal ECs and PCs overexpressing caspase-14.
html.description.abstractConclusions: Our findings indicate that caspase-14 might play a significant role in the pathogenesis of DR by accelerating retinal PC and EC death. Further investigations are required to elaborate the underlying mechanisms.


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