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    Mitochondrial Dysfunction and Adipogenic Reduction by Prohibitin Silencing in 3T3-L1 Cells

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    Authors
    Liu, Dong
    Lin, Yiming
    Kang, Ting
    Huang, Bo
    Xu, Wei
    Garcia-Barrio, Minerva
    Olatinwo, Moshood
    Matthews, Roland
    Chen, Yuqing Eugene
    Thompson, Winston E.
    Issue Date
    2012-03-30
    URI
    http://hdl.handle.net/10675.2/706
    
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    Abstract
    Increase in mitochondrial biogenesis has been shown to accompany brown and white adipose cell differentiation. Prohibitins (PHBs), comprised of two evolutionarily conserved proteins, prohibitin-1 (PHB1) and prohibitin-2 (PHB2), are present in a high molecular-weight complex in the inner membrane of mitochondria. However, little is known about the effect of mitochondrial PHBs in adipogenesis. In the present study, we demonstrate that the levels of both PHB1 and PHB2 are significantly increased during adipogenesis of 3T3-L1 preadipocytes, especially in mitochondria. Knockdown of PHB1 or PHB2 by oligonucleotide siRNA significantly reduced the expression of adipogenic markers, the accumulation of lipids and the phosphorylation of extracellular signal-regulated kinases. In addition, fragmentation of mitochondrial reticulum, loss of mitochondrial cristae, reduction of mitochondrial content, impairment of mitochondrial complex I activity and excessive production of ROS were observed upon PHB-silencing in 3T3-L1 cells. Our results suggest that PHBs are critical mediators in promoting 3T3-L1 adipocyte differentiation and may be the potential targets for obesity therapies.
    Citation
    PLoS One. 2012 Mar 30; 7(3):e34315
    ae974a485f413a2113503eed53cd6c53
    10.1371/journal.pone.0034315
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