• Login
    View Item 
    •   Home
    • Colleges & Programs
    • Medical College of Georgia (MCG)
    • Department of Cellular Biology and Anatomy
    • Department of Cellular Biology and Anatomy: Faculty Research and Presentations
    • View Item
    •   Home
    • Colleges & Programs
    • Medical College of Georgia (MCG)
    • Department of Cellular Biology and Anatomy
    • Department of Cellular Biology and Anatomy: Faculty Research and Presentations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of Scholarly CommonsCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsThis CollectionTitleAuthorsIssue DateSubmit DateSubjects

    My Account

    LoginRegister

    About

    AboutCreative CommonsAugusta University LibrariesUSG Copyright Policy

    Statistics

    Display statistics

    The Effects of Mechanical Stress on the Growth, Differentiation, and Paracrine Factor Production of Cardiac Stem Cells

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    pone.0028890.pdf
    Size:
    1.439Mb
    Format:
    PDF
    Download
    Authors
    Kurazumi, Hiroshi
    Kubo, Masayuki
    Ohshima, Mako
    Yamamoto, Yumi
    Takemoto, Yoshihiro
    Suzuki, Ryo
    Ikenaga, Shigeru
    Mikamo, Akihito
    Udo, Koichi
    Hamano, Kimikazu
    Li, Tao-Sheng
    Show allShow less
    Issue Date
    2011-12-28
    URI
    http://hdl.handle.net/10675.2/689
    
    Metadata
    Show full item record
    Abstract
    Stem cell therapies have been clinically employed to repair the injured heart, and cardiac stem cells are thought to be one of the most potent stem cell candidates. The beating heart is characterized by dynamic mechanical stresses, which may have a significant impact on stem cell therapy. The purpose of this study is to investigate how mechanical stress affects the growth and differentiation of cardiac stem cells and their release of paracrine factors. In this study, human cardiac stem cells were seeded in a silicon chamber and mechanical stress was then induced by cyclic stretch stimulation (60 cycles/min with 120% elongation). Cells grown in non-stretched silicon chambers were used as controls. Our result revealed that mechanical stretching significantly reduced the total number of surviving cells, decreased Ki-67-positive cells, and increased TUNEL-positive cells in the stretched group 24 hrs after stretching, as compared to the control group. Interestingly, mechanical stretching significantly increased the release of the inflammatory cytokines IL-6 and IL-1β as well as the angiogenic growth factors VEGF and bFGF from the cells in 12 hrs. Furthermore, mechanical stretching significantly reduced the percentage of c-kit-positive stem cells, but increased the expressions of cardiac troponin-I and smooth muscle actin in cells 3 days after stretching. Using a traditional stretching model, we demonstrated that mechanical stress suppressed the growth and proliferation of cardiac stem cells, enhanced their release of inflammatory cytokines and angiogenic factors, and improved their myogenic differentiation. The development of this in vitro approach may help elucidate the complex mechanisms of stem cell therapy for heart failure.
    Citation
    PLoS One. 2011 Dec 28; 6(12):e28890
    ae974a485f413a2113503eed53cd6c53
    10.1371/journal.pone.0028890
    Scopus Count
    Collections
    Department of Cellular Biology and Anatomy: Faculty Research and Presentations

    entitlement

    Related articles

    • Haemodynamic unloading increases the survival and affects the differentiation of cardiac stem cells after implantation into an infarcted heart.
    • Authors: Kurazumi H, Li TS, Takemoto Y, Suzuki R, Mikamo A, Guo CY, Murata T, Hamano K
    • Issue date: 2014 Jun
    • Mechanical stress induces production of angiogenic regulators in cultured human gingival and periodontal ligament fibroblasts.
    • Authors: Yoshino H, Morita I, Murota SI, Ishikawa I
    • Issue date: 2003 Aug
    • In vitro and in vivo effects of bone marrow stem cells on cardiac structure and function.
    • Authors: Xu M, Uemura R, Dai Y, Wang Y, Pasha Z, Ashraf M
    • Issue date: 2007 Feb
    • Role of epidermal growth factor and its receptor in mechanical stress-induced differentiation of human periodontal ligament cells in vitro.
    • Authors: Matsuda N, Yokoyama K, Takeshita S, Watanabe M
    • Issue date: 1998 Dec
    • Mechanical Stress Promotes Maturation of Human Myocardium From Pluripotent Stem Cell-Derived Progenitors.
    • Authors: Ruan JL, Tulloch NL, Saiget M, Paige SL, Razumova MV, Regnier M, Tung KC, Keller G, Pabon L, Reinecke H, Murry CE
    • Issue date: 2015 Jul

    Related items

    Showing items related by title, author, creator and subject.

    • Thumbnail

      Associations Between Social Determinants of Health and Net Stress

      Lassiter, Debbie Jo; Department of Physiological & Technological Nursing (Augusta University, 2021-12)
      Stress is known to cause dire physical and mental health outcomes. Extant stress research has lacked a subjective rapid screening tool for further evaluation of high risk individuals. Net stress is a new construct developed in this study as the calculation of an individual’s perceived average stress level compared with their perceived healthy stress level. Measuring net stress provides an additional construct to identify health disparities among individuals at potential risk for mental and physical illness. The two aims were first to develop the net stress construct and secondly to examine and evaluate the relationships between net stress and Social Determinants of Health (SDoH) domain variables from the 2018 Stress in AmericaTM survey developed by APA and The Harris Poll survey and analytics group. The five SDoH domains developed by Healthy People 2020 were Neighborhood and Built Environment, Health and Health Care, Social and Community Context, Education, and Economic Stability. The methods included an exploratory, secondary analysis which included multiple regression to test whether net stress would respond predictably as a valid, new construct within the sample from the survey (N = 3,091). Net stress was regressed on the groups of variables for each domain, then on a model with all variables to determine if there were substantial differences in how net stress responded. Conclusions for this study were that four of the five domains, excepting Education, had substantial associations (0.25 standard deviation) with net stress. Using slightly less restrictive criteria, net stress was associated with variables from all five SDoH domains. Results were that net stress responded as a valid, new construct within this analysis. This study found that individuals with the following circumstances had substantially higher net stress levels: larger household size up to eight residents, single parent family structure, having no insurance, having a low perceived health level, being of bisexual orientation, being female, and having low household income. The Economic Stability domain impacted all other domains. Implications for future research, healthcare practice, nursing theory, and policy were discussed.
    • Thumbnail

      The Beeper [2000 Vol. 10, No. 14]

      Baker, Toni; Steele, Deborah; Simkins, Sally L.; Deriso, Christine Hurley; Deriso, Christine Hurley; Steele, Deborah; Division of Institutional Relations; Division of Strategic Support (Medical College of Georgia, 2000-07-13)
      Features this week include: MCG working to place employees whose jobs have been eliminated; Program immerses high school students in dentistry; Ms. Garlington honored by alma mater; MCG wins fund-raising award; Stress an inevitable byproduct of change, psychologist says; Set SMART goals to lighten your load and reduce stress; 'Alex' helps nursing students respond to emergencies; MCG physicians lend skills to SWAT team; Board of Regents names presidential search committee; Tips help parents minimize sibling rivalry; Parenting is hands-on activity, child psychiatrist says.
    • Thumbnail

      The Beeper [1982 Vol. 3, No. 2]

      Donnelly, John; Roth, Mandy; Wilson, Bob; Division of Institutional Relations; Division of Strategic Support (Medical College of Georgia, 1982-01-13)
      The Beeper, a campus publication, is produced weekly featuring the Medical College of Georgia’s faculty, staff, and students activities, research, and accomplishments; including campus announcements and news.
    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.