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dc.contributor.authorBish, Lawrence T.
dc.contributor.authorGeorge, Isaac
dc.contributor.authorMaybaum, Simon
dc.contributor.authorYang, Jonathan
dc.contributor.authorChen, Jonathan M.
dc.contributor.authorSweeney, H. Lee
dc.contributor.editorMcNeil, Paul L.
dc.date.accessioned2012-10-26T16:29:30Z
dc.date.available2012-10-26T16:29:30Z
dc.date.issued2011-09-13en_US
dc.identifier.citationPLoS One. 2011 Sep 13; 6(9):e23818en_US
dc.identifier.issn1932-6203en_US
dc.identifier.pmid21931616en_US
dc.identifier.doi10.1371/journal.pone.0023818en_US
dc.identifier.urihttp://hdl.handle.net/10675.2/675
dc.description.abstractBackground: Myostatin is a negative regulator of skeletal muscle mass whose activity is upregulated in adult heart failure (HF); however, its role in congenital heart disease (CHD) is unknown.
dc.description.abstractMethods: We studied myostatin and IGF-1 expression via Western blot in cardiac tissue at varying degrees of myocardial dysfunction and after biventricular support in CHD by collecting myocardial biopsies from four patient cohorts: A) adult subjects with no known cardiopulmonary disease (left ventricle, LV), (Adult Normal), (n = 5); B) pediatric subjects undergoing congenital cardiac surgery with normal RV size and function (right ventricular outflow tract, RVOT), (n = 3); C) pediatric subjects with worsening but hemodynamically stable LV failure [LV and right ventricle (LV, RV,)] with biopsy collected at the time of orthotopic heart transplant (OHT), (n = 7); and D) pediatric subjects with decompensated bi-ventricular failure on BiVAD support with biopsy collected at OHT (LV, RV, BiVAD), (n = 3).
dc.description.abstractResults: The duration of HF was longest in OHT patients compared to BIVAD. The duration of BiVAD support was 4.361.9 days. Myostatin expression was significantly increased in LV-OHT compared to RV-OHT and RVOT, and was increased more than double in decompensated biventricular HF (BiVAD) compared to both OHT and RVOT. An increased myostatin/IGF-1 ratio was associated with ventricular dysfunction.
dc.description.abstractConclusions: Myostatin expression in increased in CHD, and the myostatin/IGF-1 ratio increases as ventricular function deteriorates. Future investigation is necessary to determine if restoration of the physiologic myostatin/IGF-1 ratio has therapeutic potential in HF.
dc.rightsBish et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.subjectResearch Articleen_US
dc.subjectBiologyen_US
dc.subjectBiochemistryen_US
dc.subjectProteinsen_US
dc.subjectGrowth Factorsen_US
dc.subjectGeneticsen_US
dc.subjectGene Expressionen_US
dc.subjectMolecular Cell Biologyen_US
dc.subjectCell Growthen_US
dc.subjectMedicineen_US
dc.subjectCardiovascularen_US
dc.subjectCardiomyopathiesen_US
dc.subjectCongenital Heart Diseaseen_US
dc.subjectHeart Failureen_US
dc.subjectPediatric Cardiologyen_US
dc.subjectSurgeryen_US
dc.subjectCardiovascular Surgeryen_US
dc.subjectPediatric Surgeryen_US
dc.titleMyostatin Is Elevated in Congenital Heart Disease and After Mechanical Unloadingen_US
dc.typeArticleen_US
dc.identifier.pmcidPMC3172210en_US
dc.contributor.corporatenameDepartment of Cellular Biology and Anatomy
dc.contributor.corporatenameCollege of Graduate Studies
refterms.dateFOA2019-04-10T00:31:11Z
html.description.abstractBackground: Myostatin is a negative regulator of skeletal muscle mass whose activity is upregulated in adult heart failure (HF); however, its role in congenital heart disease (CHD) is unknown.
html.description.abstractMethods: We studied myostatin and IGF-1 expression via Western blot in cardiac tissue at varying degrees of myocardial dysfunction and after biventricular support in CHD by collecting myocardial biopsies from four patient cohorts: A) adult subjects with no known cardiopulmonary disease (left ventricle, LV), (Adult Normal), (n = 5); B) pediatric subjects undergoing congenital cardiac surgery with normal RV size and function (right ventricular outflow tract, RVOT), (n = 3); C) pediatric subjects with worsening but hemodynamically stable LV failure [LV and right ventricle (LV, RV,)] with biopsy collected at the time of orthotopic heart transplant (OHT), (n = 7); and D) pediatric subjects with decompensated bi-ventricular failure on BiVAD support with biopsy collected at OHT (LV, RV, BiVAD), (n = 3).
html.description.abstractResults: The duration of HF was longest in OHT patients compared to BIVAD. The duration of BiVAD support was 4.361.9 days. Myostatin expression was significantly increased in LV-OHT compared to RV-OHT and RVOT, and was increased more than double in decompensated biventricular HF (BiVAD) compared to both OHT and RVOT. An increased myostatin/IGF-1 ratio was associated with ventricular dysfunction.
html.description.abstractConclusions: Myostatin expression in increased in CHD, and the myostatin/IGF-1 ratio increases as ventricular function deteriorates. Future investigation is necessary to determine if restoration of the physiologic myostatin/IGF-1 ratio has therapeutic potential in HF.


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