Modulation of Syndecan-1 Shedding after Hemorrhagic Shock and Resuscitation
dc.contributor.author | Haywood-Watson, Ricky J. | |
dc.contributor.author | Holcomb, John B. | |
dc.contributor.author | Gonzalez, Ernest A. | |
dc.contributor.author | Peng, Zhanglong | |
dc.contributor.author | Pati, Shibani | |
dc.contributor.author | Park, Pyong Woo | |
dc.contributor.author | Wang, WeiWei | |
dc.contributor.author | Zaske, Ana Maria | |
dc.contributor.author | Menge, Tyler | |
dc.contributor.author | Kozar, Rosemary A. | |
dc.contributor.editor | McNeil, Paul L. | |
dc.date.accessioned | 2012-10-26T16:29:29Z | |
dc.date.available | 2012-10-26T16:29:29Z | |
dc.date.issued | 2011-08-19 | en_US |
dc.identifier.citation | PLoS One. 2011 Aug 19; 6(8):e23530 | en_US |
dc.identifier.issn | 1932-6203 | en_US |
dc.identifier.pmid | 21886795 | en_US |
dc.identifier.doi | 10.1371/journal.pone.0023530 | en_US |
dc.identifier.uri | http://hdl.handle.net/10675.2/670 | |
dc.description.abstract | The early use of fresh frozen plasma as a resuscitative agent after hemorrhagic shock has been associated with improved survival, but the mechanism of protection is unknown. Hemorrhagic shock causes endothelial cell dysfunction and we hypothesized that fresh frozen plasma would restore endothelial integrity and reduce syndecan-1 shedding after hemorrhagic shock. A prospective, observational study in severely injured patients in hemorrhagic shock demonstrated significantly elevated levels of syndecan-1 (554±93 ng/ml) after injury, which decreased with resuscitation (187±36 ng/ml) but was elevated compared to normal donors (27±1 ng/ml). Three pro-inflammatory cytokines, interferon-γ, fractalkine, and interleukin-1β, negatively correlated while one anti-inflammatory cytokine, IL-10, positively correlated with shed syndecan-1. These cytokines all play an important role in maintaining endothelial integrity. An in vitro model of endothelial injury then specifically examined endothelial permeability after treatment with fresh frozen plasma orlactated Ringers. Shock or endothelial injury disrupted junctional integrity and increased permeability, which was improved with fresh frozen plasma, but not lactated Ringers. Changes in endothelial cell permeability correlated with syndecan-1 shedding. These data suggest that plasma based resuscitation preserved endothelial syndecan-1 and maintained endothelial integrity, and may help to explain the protective effects of fresh frozen plasma after hemorrhagic shock. | |
dc.rights | Haywood-Watson et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | en_US |
dc.subject | Research Article | en_US |
dc.subject | Biology | en_US |
dc.subject | Molecular Cell Biology | en_US |
dc.subject | Medicine | en_US |
dc.subject | Anatomy and Physiology | en_US |
dc.subject | Immune Physiology | en_US |
dc.subject | Cytokines | en_US |
dc.subject | Diagnostic Medicine | en_US |
dc.subject | Clinical Laboratory Sciences | en_US |
dc.subject | Transfusion Medicine | en_US |
dc.subject | Surgery | en_US |
dc.subject | Trauma Surgery | en_US |
dc.title | Modulation of Syndecan-1 Shedding after Hemorrhagic Shock and Resuscitation | en_US |
dc.type | Article | en_US |
dc.identifier.pmcid | PMC3158765 | en_US |
dc.contributor.corporatename | Department of Cellular Biology and Anatomy | |
dc.contributor.corporatename | College of Graduate Studies | |
refterms.dateFOA | 2019-04-10T00:30:41Z | |
html.description.abstract | The early use of fresh frozen plasma as a resuscitative agent after hemorrhagic shock has been associated with improved survival, but the mechanism of protection is unknown. Hemorrhagic shock causes endothelial cell dysfunction and we hypothesized that fresh frozen plasma would restore endothelial integrity and reduce syndecan-1 shedding after hemorrhagic shock. A prospective, observational study in severely injured patients in hemorrhagic shock demonstrated significantly elevated levels of syndecan-1 (554±93 ng/ml) after injury, which decreased with resuscitation (187±36 ng/ml) but was elevated compared to normal donors (27±1 ng/ml). Three pro-inflammatory cytokines, interferon-γ, fractalkine, and interleukin-1β, negatively correlated while one anti-inflammatory cytokine, IL-10, positively correlated with shed syndecan-1. These cytokines all play an important role in maintaining endothelial integrity. An in vitro model of endothelial injury then specifically examined endothelial permeability after treatment with fresh frozen plasma orlactated Ringers. Shock or endothelial injury disrupted junctional integrity and increased permeability, which was improved with fresh frozen plasma, but not lactated Ringers. Changes in endothelial cell permeability correlated with syndecan-1 shedding. These data suggest that plasma based resuscitation preserved endothelial syndecan-1 and maintained endothelial integrity, and may help to explain the protective effects of fresh frozen plasma after hemorrhagic shock. |