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dc.contributor.authorZhang, Zhuo
dc.contributor.authorZou, Jun
dc.contributor.authorWang, Guo-Kun
dc.contributor.authorZhang, Jun-Tao
dc.contributor.authorHuang, Shuang
dc.contributor.authorQin, Yong-Wen
dc.contributor.authorJing, Qing
dc.date.accessioned2012-10-26T16:27:00Z
dc.date.available2012-10-26T16:27:00Z
dc.date.issued2011-02-1en_US
dc.identifier.citationNucleic Acids Res. 2011 May 1; 39(10):4387-4395en_US
dc.identifier.issn1362-4962en_US
dc.identifier.pmid21288881en_US
dc.identifier.doi10.1093/nar/gkr020en_US
dc.identifier.urihttp://hdl.handle.net/10675.2/656
dc.description.abstractMicroRNAs are endogenous small RNA molecules that regulate gene expression. Although the biogenesis of microRNAs and their regulation have been thoroughly elucidated, the degradation of microRNAs has not been fully understood. Here by using the pulseâ chase approach, we performed the direct measurement of microRNA lifespan. Five representative microRNAs demonstrated a general feature of relatively long lifespan. However, the decay dynamic varies considerably between these individual microRNAs. Mutation analysis of miR-29b sequence revealed that uracils at nucleotide position 9â 11 are required for its rapid decay, in that both specific nucleotides and their position are critical. The effect of uracil-rich element on miR-29b decay dynamic occurs in duplex but not in single strand RNA. Moreover, analysis of published data on microRNA expression profile during development reveals that a substantial subset of microRNAs with the uracil-rich sequence tends to be down-regulated compared to those without the sequence. Among them, Northern blotting shows that miR-29c and fruit fly bantam possess a relatively rapid turnover rate. The effect of uracil-rich sequence on microRNA turnover depends on the sequence context. The present work indicates that microRNAs contain sequence information in the middle region besides the sequence element at both ends.
dc.rights© The Author(s) 2011. Published by Oxford University Press.en_US
dc.subjectRNAen_US
dc.titleUracils at nucleotide position 9â 11 are required for the rapid turnover of miR-29 familyen_US
dc.typeArticleen_US
dc.identifier.pmcidPMC3105410en_US
dc.contributor.corporatenameDepartment of Biochemistry and Molecular Biology
refterms.dateFOA2019-04-10T00:28:25Z
html.description.abstractMicroRNAs are endogenous small RNA molecules that regulate gene expression. Although the biogenesis of microRNAs and their regulation have been thoroughly elucidated, the degradation of microRNAs has not been fully understood. Here by using the pulseâ chase approach, we performed the direct measurement of microRNA lifespan. Five representative microRNAs demonstrated a general feature of relatively long lifespan. However, the decay dynamic varies considerably between these individual microRNAs. Mutation analysis of miR-29b sequence revealed that uracils at nucleotide position 9â 11 are required for its rapid decay, in that both specific nucleotides and their position are critical. The effect of uracil-rich element on miR-29b decay dynamic occurs in duplex but not in single strand RNA. Moreover, analysis of published data on microRNA expression profile during development reveals that a substantial subset of microRNAs with the uracil-rich sequence tends to be down-regulated compared to those without the sequence. Among them, Northern blotting shows that miR-29c and fruit fly bantam possess a relatively rapid turnover rate. The effect of uracil-rich sequence on microRNA turnover depends on the sequence context. The present work indicates that microRNAs contain sequence information in the middle region besides the sequence element at both ends.


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