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    Uracils at nucleotide position 9â 11 are required for the rapid turnover of miR-29 family

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    Authors
    Zhang, Zhuo
    Zou, Jun
    Wang, Guo-Kun
    Zhang, Jun-Tao
    Huang, Shuang
    Qin, Yong-Wen
    Jing, Qing
    Issue Date
    2011-02-1
    URI
    http://hdl.handle.net/10675.2/656
    
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    Abstract
    MicroRNAs are endogenous small RNA molecules that regulate gene expression. Although the biogenesis of microRNAs and their regulation have been thoroughly elucidated, the degradation of microRNAs has not been fully understood. Here by using the pulseâ chase approach, we performed the direct measurement of microRNA lifespan. Five representative microRNAs demonstrated a general feature of relatively long lifespan. However, the decay dynamic varies considerably between these individual microRNAs. Mutation analysis of miR-29b sequence revealed that uracils at nucleotide position 9â 11 are required for its rapid decay, in that both specific nucleotides and their position are critical. The effect of uracil-rich element on miR-29b decay dynamic occurs in duplex but not in single strand RNA. Moreover, analysis of published data on microRNA expression profile during development reveals that a substantial subset of microRNAs with the uracil-rich sequence tends to be down-regulated compared to those without the sequence. Among them, Northern blotting shows that miR-29c and fruit fly bantam possess a relatively rapid turnover rate. The effect of uracil-rich sequence on microRNA turnover depends on the sequence context. The present work indicates that microRNAs contain sequence information in the middle region besides the sequence element at both ends.
    Citation
    Nucleic Acids Res. 2011 May 1; 39(10):4387-4395
    ae974a485f413a2113503eed53cd6c53
    10.1093/nar/gkr020
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