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dc.contributor.authorMyllymaki, Satu Marja
dc.contributor.authorTeravainen, Terhi Piritta
dc.contributor.authorManninen, Aki
dc.contributor.editorMei, Lin
dc.date.accessioned2012-10-26T16:26:59Z
dc.date.available2012-10-26T16:26:59Z
dc.date.issued2011-05-6en_US
dc.identifier.citationPLoS One. 2011 May 6; 6(5):e19453en_US
dc.identifier.issn1932-6203en_US
dc.identifier.pmid21573123en_US
dc.identifier.doi10.1371/journal.pone.0019453en_US
dc.identifier.urihttp://hdl.handle.net/10675.2/653
dc.description.abstractBackground: Formation of apical compartments underlies the morphogenesis of most epithelial organs during development. The extracellular matrix (ECM), particularly the basement membrane (BM), plays an important role in orienting the apico-basal polarity and thereby the positioning of apical lumens. Integrins have been recognized as essential mediators of matrix-derived polarity signals. The importance of b1-integrins in epithelial polarization is well established but the significance of the accompanying a-subunits have not been analyzed in detail.
dc.description.abstractPrincipal Findings: Here we demonstrate that two distinct integrin-dependent pathways regulate formation of apical lumens to ensure robust apical membrane biogenesis under different microenvironmental conditions; 1) a2b1- and a6b4- integrins were required to establish a basal cue that depends on Rac1-activity and guides apico-basal cell polarization. 2) a3b1-integrins were implicated in positioning of mitotic spindles in cysts, a process that is essential for Cdc42-driven epithelial hollowing.
dc.description.abstractSignificance: Identification of the separate processes driven by particular integrin receptors clarifies the functional hierarchies between the different integrins co-expressed in epithelial cells and provides valuable insight into the complexity of cell-ECM interactions thereby guiding future studies addressing the molecular basis of epithelial morphogenesis during development and disease.
dc.rightsMyllymäki et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.subjectResearch Articleen_US
dc.subjectBiologyen_US
dc.subjectMolecular Cell Biologyen_US
dc.subjectCell Adhesionen_US
dc.subjectIntegrinsen_US
dc.subjectCellular Structuresen_US
dc.subjectCytoskeletonen_US
dc.subjectCellular Typesen_US
dc.subjectEpithelial Cellsen_US
dc.subjectExtracellular Matrixen_US
dc.subjectBasement Membraneen_US
dc.subjectExtracellular Matrix Adhesionsen_US
dc.subjectExtracellular Matrix Compositionen_US
dc.subjectIntegrinsen_US
dc.titleTwo Distinct Integrin-Mediated Mechanisms Contribute to Apical Lumen Formation in Epithelial Cellsen_US
dc.typeArticleen_US
dc.identifier.pmcidPMC3089628en_US
dc.contributor.corporatenameDepartment of Neurology
dc.contributor.corporatenameCollege of Graduate Studies
refterms.dateFOA2019-04-10T00:28:07Z
html.description.abstractBackground: Formation of apical compartments underlies the morphogenesis of most epithelial organs during development. The extracellular matrix (ECM), particularly the basement membrane (BM), plays an important role in orienting the apico-basal polarity and thereby the positioning of apical lumens. Integrins have been recognized as essential mediators of matrix-derived polarity signals. The importance of b1-integrins in epithelial polarization is well established but the significance of the accompanying a-subunits have not been analyzed in detail.
html.description.abstractPrincipal Findings: Here we demonstrate that two distinct integrin-dependent pathways regulate formation of apical lumens to ensure robust apical membrane biogenesis under different microenvironmental conditions; 1) a2b1- and a6b4- integrins were required to establish a basal cue that depends on Rac1-activity and guides apico-basal cell polarization. 2) a3b1-integrins were implicated in positioning of mitotic spindles in cysts, a process that is essential for Cdc42-driven epithelial hollowing.
html.description.abstractSignificance: Identification of the separate processes driven by particular integrin receptors clarifies the functional hierarchies between the different integrins co-expressed in epithelial cells and provides valuable insight into the complexity of cell-ECM interactions thereby guiding future studies addressing the molecular basis of epithelial morphogenesis during development and disease.


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