HER2 Targeted Molecular MR Imaging Using a De Novo Designed Protein Contrast Agent
Authors
Qiao, JingjuanLi, Shunyi
Wei, Lixia
Jiang, Jie
Long, Robert
Mao, Hui
Wei, Ling
Wang, Liya
Yang, Hua
Grossniklaus, Hans E.
Liu, Zhi-Ren
Yang, Jenny J.
Issue Date
2011-03-24
Metadata
Show full item recordAbstract
The application of magnetic resonance imaging (MRI) to non-invasively assess disease biomarkers has been hampered by the lack of desired contrast agents with high relaxivity, targeting capability, and optimized pharmacokinetics. We have developed a novel MR imaging probe targeting to HER2, a biomarker for various cancer types and a drug target for anti-cancer therapies. This multimodal HER20targeted MR imaging probe integrates a de novo designed protein contrast agent with a high affinity HER2 affibody and a near IR fluorescent dye. Our probe can differentially monitor tumors with different expression levels of HER2 in both human cell lines and xenograft mice models. In addition to its 100-fold higher dose efficiency compared to clinically approved non-targeting contrast agent DTPA, our developed agent also exhibits advantages in crossing the endothelial boundary, tissue distribution, and tumor tissue retention over reported contrast agents as demonstrated by even distribution of the imaging probe across the entire tumor mass. This contrast agent will provide a powerful tool for quantitative assessment of molecular markers, and improved resolution for diagnosis, prognosis and drug discovery.Citation
PLoS One. 2011 Mar 24; 6(3):e18103ae974a485f413a2113503eed53cd6c53
10.1371/journal.pone.0018103
Scopus Count
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