Show simple item record

dc.contributor.authorDaoudi, Asma
dc.date.accessioned2024-02-08T20:36:10Z
dc.date.available2024-02-08T20:36:10Z
dc.date.issued2015-04-25
dc.identifier.urien
dc.identifier.urihttp://hdl.handle.net/10675.2/625022
dc.description.abstractB i s p h o s p h o n a t e - R e l a t e d O s t e o n e c r o s i s o f t h e J a w (BRONJ) is a n u n t r e a t a b l e d i s e a s e c h a r a c t e r i z e d b y t h e p r e s e n c e o f e x p o s e d , n o n - v i t a l b o n e in t h e o r a l c a v i t y , f o l l o w i n g d e n t a l p r o c e d u r e s w i t h a h i s t o r y o f b i s p h o s p h o n a t e (BP) t h e r a p y . T h i s s t u d y c o m p a r e d t w o r e g i m e n s o f z o l e d r o n i c a c i d (ZA) r e g i m e n s w i t h r e s p e c t t o t h e i n d u c t i o n o f clinical f e a t u r e s o f BRONJ. W e h y p o t h e s i z e d t h a t , in t h e t r e a t e d a n i m a l s in b o t h m o d e l s , 1 ) a l v e o l a r b o n e w o u l d s h o w i m p a i r e d o s t e o c l a s t ( b o n e - r e m o v i n g cell) a c t i v i t y , 2 ) t h i s i m p a i r m e n t w o u l d b e m o r e p r o f o u n d a t h i g h e r d o s e s o f BPs, a n d 3 ) t h e r e w o u l d b e a n i n c r e a s e in m i c r o b i a l c o l o n i z a t i o n a t t h e e x t r a c t i o n s i t e . T h e s t u d y i n v o l v e d 1 0 0 f e m a l e S p r a g u e - D a w l e y r a t s : 3 0 w e r e t r e a t e d w i t h 8 0 p g / k g o f i n t r a v e n o u s ZA w e e k l y f o r 1 3 w e e k s , 2 0 w i t h 1 5 0 p g / k g ZA d a i l y f o r 1 6 c o n s e c u t i v e d a y s , a n d 5 0 w i t h c o r r e s p o n d i n g s a l i n e i n j e c t i o n s . T h e i n j e c t i o n s w e r e f o l l o w e d b y e x t r a c t i o n o f t h e f i r s t a n d s e c o n d m o l a r t e e t h ( e x t r a c t i o n h a s b e e n k n o w n t o t r i g g e r b o n e n e c r o s i s in b i s p h o s p h o n a t e - t r e a t e d p a t i e n t s ) . W e e x a m i n e d o s t e o c l a s t a n d b a c t e r i a l d i s t r i b u t i o n a t o n e a n d e i g h t w e e k s p o s t e x t r a c t i o n , u s i n g T a r t r a t e - R e s i s t a n t Acid P h o s p h a t a s e (TRAP) a n d B r o w n a n d B r e n n s t a i n i n g , r e s p e c t i v e l y . T h e TRAP r e s u l t s i n d i c a t e d a s i g n i f i c a n t d e c l i n e in o s t e o c l a s t a c t i v i t y in t h e t r e a t m e n t g r o u p s a t 1 w e e k p o s t - e x t r a c t i o n c o m p a r e d t o t h e c o n t r o l s . W h e n c o m p a r e d t o t h e clinical d o s e , t h e h i g h e r d o s e h a d m o r e p r o n o u n c e d a d e c l i n e in t h e o s t e o c l a s t r e c r u i t m e n t in t h e t r e a t e d a n i m a l s a t 8 w e e k s p o s t - e x t r a c t i o n s . T h e r e w a s n o s i g n i f i c a n t d i f f e r e n c e in b a c t e r i a l c o l o n i z a t i o n b e t w e e n g r o u p s ; h o w e v e r , w e d e t e c t e d a n i n c r e a s e in f u n g a l c o l o n i z a t i o n in t h e t r e a t e d a n i m a l s c o m p a r e d t o t h e c o n t r o l s . T h e r e s u l t s o f t h i s s t u d y s u g g e s t t h e l e g i t i m a c y o f c o n s i d e r i n g b o t h a n i m a l m o d e l s f o r f u r t h e r i n v e s t i g a t i o n o f BRONJ m e c h a n i s m s .en_US
dc.language.isoen_USen_US
dc.publisherAugusta Universityen_US
dc.rightsCopyright protected. Unauthorized reproduction or use beyond the exceptions granted by the Fair Use clause of U.S. Copyright law may violate federal law.en_US
dc.titleH i s t o l o g i c a l A n a l y s i s o f M i c r o b i a l C o l o n i z a t i o n a n d O s t e o c l a s t i c A c t i v i t y i n B i s p h o s p h o n a t e - T r e a t e d R a t sen_US
dc.typeThesisen_US
dc.contributor.departmentAugusta State University Honors Thesisen_US
dc.contributor.corporatenameAugusta State University Honors Thesisen_US
dc.description.advisorElsalanty, Mohammed
dc.description.committeeN/A
dc.description.degreeN/Aen_US
dc.embargoen
refterms.dateFOA2024-02-08T20:36:12Z
dc.description.embargo2015-04-25


Files in this item

Thumbnail
Name:
daoudi.pdf
Size:
23.84Mb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record