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dc.contributor.authorBradford, jennifer W
dc.contributor.authorKorkaya, Ahment K
dc.contributor.authorFischer, Jeffrey
dc.contributor.authorPeppers, Anthony
dc.contributor.authorCrosson, Sean
dc.contributor.authorRayamajhi, Manira
dc.contributor.authorMiao, Edward A
dc.contributor.authorBaldwin Jr, Albert S
dc.date.accessioned2024-02-08T18:46:14Z
dc.date.available2024-02-08T18:46:14Z
dc.identifier.urihttp://hdl.handle.net/10675.2/625018
dc.description.abstractHere, we describe the production and characterization of a novel p65fl/fl/LysMCre mouse model, which lacks canonical nuclear factor-kappaB member RelA/p65 (indicated as p65 hereafter) in bone marrow-derived macrophages. Cultured bone marrow-derived macrophages that lack p65 protein reveal NF-κB signaling deficiencies, a reduction in phagocytic ability, and reduced ability to produce nitrites. Despite abnormal bone marrow-derived macrophage function, p65fl/fl/LysMCre mice do not exhibit differences in naïve systemic immune profiles or colony forming units and time to death following Salmonella infection as compared to controls. Additionally, p65fl/fl/LysMCre mice, especially females, display splenomegaly, but no other obvious physical or behavioral differences as compared to control animals. As bone marrow-derived macrophages from this transgenic model are almost completely devoid of canonical nuclear factor-kappaB pathway member p65, this model has the potential for being very useful in investigating bone marrow-derived macrophage NF-kappaB signaling in diverse biological and biomedical studies.en_US
dc.language.isoen_USen_US
dc.publisherSage Journalsen_US
dc.subjectAnimal modelen_US
dc.subjectbone marrow-derived macrophageen_US
dc.subjectcytokineen_US
dc.subjectinnate immunityen_US
dc.subjectnuclear factor-kappaBen_US
dc.titleProduction of a p65fl/fl/LysMCre mouse model with dysfunctional NF-kappaB signaling in bone marrow-derived macrophagesen_US
dc.typeAbstracten_US
dc.typeArticleen_US
dc.contributor.departmentAugusta Universityen_US
dc.identifier.journalInnate Immunityen_US
dc.description.degreeBiologyen_US
refterms.dateFOA2024-02-08T18:46:15Z


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