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dc.contributor.authorSahajpal, Nikhil S.
dc.contributor.authorMondal, Ashis K.
dc.contributor.authorAnanth, Sudha
dc.contributor.authorNjau, Allan
dc.contributor.authorFulzele, Sadanand
dc.contributor.authorAhaluwalia, Pankaj
dc.contributor.authorChaubey, Alka
dc.contributor.authorHegde, Madhuri
dc.contributor.authorRojiani, Amyn M.
dc.contributor.authorKolhe, Ravindra
dc.date.accessioned2022-03-09T20:17:39Z
dc.date.available2022-03-09T20:17:39Z
dc.date.issued2021-06-29
dc.identifier.issn2374-2895
dc.identifier.doi10.1177/23742895211023948
dc.identifier.urihttp://hdl.handle.net/10675.2/624250
dc.description.abstractThe COVID-19 pandemic, caused by severe acute respiratory syndrome coronavirus 2, led to unprecedented demands assigned to clinical diagnostic laboratories worldwide, forcing them to make significant changes to their regular workflow as they adapted to new diagnostic tests and sample volumes. Herein, we summarize the modifications/adaptation the laboratory had to exercise to cope with rapidly evolving situations in the current pandemic. In the first phase of the pandemic, the laboratory validated 2 reverse transcription polymerase chain reaction–based assays to test ∼1000 samples/day and rapidly modified procedures and validated various preanalytical and analytical steps to overcome the supply chain constraints that would have otherwise derailed testing efforts. Further, the pooling strategy was validated for wide-scale population screening using nasopharyngeal swab samples and saliva samples. The translational research arm of the laboratory pursued several initiatives to understand the variable clinical manifestations that this virus presented in the population. The phylogenetic evolution of the virus was investigated using next-generation sequencing technology. The laboratory has initiated the formation of a consortium that includes groups investigating genomes at the level of large structural variants, using genome optical mapping via this collaborative global effort. This article summarizes our journey as the laboratory has sought to adapt and continue to positively contribute to the unprecedented demands and challenges of this rapidly evolving pandemic.en_US
dc.description.sponsorshipPart of this project has been funded in the lab by the National Institute of Allergy and Infectious Diseases, a component of the NIH, Department of Health and Human Services, under contract 75N93019C00052en_US
dc.language.isoenen_US
dc.publisherSageen_US
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectPathology and Forensic Medicineen_US
dc.subjectCOVID-19, pandemic, clinical laboratory, testing, pooling strategyen_US
dc.titleMaking a Difference: Adaptation of the Clinical Laboratory in Response to the Rapidly Evolving COVID-19 Pandemicen_US
dc.typeArticleen_US
dc.identifier.eissn2374-2895
dc.contributor.departmentDepartment of Pathology; Center for Healthy Agingen_US
dc.identifier.journalAcademic Pathologyen_US
dc.identifier.pii10.1177/23742895211023948
dc.source.journaltitleAcademic Pathology
dc.source.volume8
dc.source.beginpage237428952110239
refterms.dateFOA2022-03-09T20:17:39Z


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