Next-Generation Sequencing (NGS) in COVID-19: A Tool for SARS-CoV-2 Diagnosis, Monitoring New Strains and Phylodynamic Modeling in Molecular Epidemiology
Authors
John, GoldinSahajpal, Nikhil Shri
Mondal, Ashis K.
Ananth, Sudha
Williams, Colin
Chaubey, Alka
Rojiani, Amyn M.
Kolhe, Ravindra
Issue Date
2021-07-30
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This review discusses the current testing methodologies for COVID-19 diagnosis and explores next-generation sequencing (NGS) technology for the detection of SARS-CoV-2 and monitoring phylogenetic evolution in the current COVID-19 pandemic. The review addresses the development, fundamentals, assay quality control and bioinformatics processing of the NGS data. This article provides a comprehensive review of the obstacles and opportunities facing the application of NGS technologies for the diagnosis, surveillance, and study of SARS-CoV-2 and other infectious diseases. Further, we have contemplated the opportunities and challenges inherent in the adoption of NGS technology as a diagnostic test with real-world examples of its utility in the fight against COVID-19.Affiliation
Department of Pathologyae974a485f413a2113503eed53cd6c53
10.3390/cimb43020061
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Except where otherwise noted, this item's license is described as https://creativecommons.org/licenses/by/4.0/