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dc.contributor.authorRobinson, Rebekah
dc.date.accessioned2021-12-03T17:05:16Z
dc.date.available2021-12-03T17:05:16Z
dc.date.issued2022-05
dc.identifier.urihttp://hdl.handle.net/10675.2/624151
dc.description.abstractRecent studies in our lab have demonstrated that interleukin 6 (IL-6) signaling through its soluble receptor (IL-6 “trans-signaling”) plays a role in the pathogenesis of diabetic retinopathy (DR). The major goals of this project are to expand upon this knowledge by evaluating the effects of trans-signaling on retinal endothelial gene expression and permeability, Müller glia mitochondrial dysfunction, and the murine vitreous proteome. This study demonstrates that (i) trans-signaling significantly upregulates genes related to inflammation and leukocyte recruitment in retinal endothelial cells, and (ii) that the changes to endothelial permeability induced by IL-6 trans-signaling are partially mediated by less of -catenin expression. It also demonstrates that (iii) IL-6 trans-signaling induces Müller glia mitochondrial dysfunction and apoptosis in a glucose-dependent manner, and (iv) that inhibition of IL-6 trans-signaling in a mouse model of DR prevents diabetes-induced alterations to the vitreous humor proteome. Together, these data emphasize the importance of IL-6 trans-signaling in DR pathology and its potential as a novel therapeutic target.
dc.publisherAugusta University
dc.subjectMolecular biology
dc.subjectCellular biology
dc.subjectdiabetic retinopathy, endothelial permeability, interleukin 6, Müller glia, trans-signaling, vitreous
dc.titleMOLECULAR MECHANISMS OF INTERLEUKIN-6 TRANS-SIGNALING MEDIATED EFFECTS ON ENDOTHELIAL PERMEABILITY, MÜLLER GLIA DYSFUNCTION, AND VITREOUS PROTEOMIC ALTERATIONS IN DIABETIC RETINOPATHY
dc.typedissertationen_US
dc.contributor.departmentBiomedical Sciences
dc.language.rfc3066en
dc.date.updated2021-12-03T17:05:17Z
dc.description.advisorSharma, Shruti
dc.description.committeeSharma, Ashok
dc.description.committeeBollag, Roni
dc.description.committeeXu, Hongyan
dc.description.committeePatel, Nikhil
dc.description.committeeLukowski, Zachary
dc.description.degreePh.D.
dc.description.embargo12/03/2023


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