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dc.contributor.authorBlackburn, Helena
dc.date.accessioned2021-03-18T19:18:08Z
dc.date.available2021-03-18T19:18:08Z
dc.date.issued2020-12
dc.identifier.urihttp://hdl.handle.net/10675.2/623914
dc.descriptionRecord is embargoed until 12/10/2022en_US
dc.description.abstractTinagl1 is a secreted protein found in the basement membrane under epithelial cells. The LeMosy lab previously showed that tinagl1 knockdowns resulted in abnormal spinal development and heart orientation during zebrafish development. These data, together with changes in length of motile cilia, suggested that tinagl1 is involved in cilia function during development. The mechanism of this interaction is unknown, and it is unclear whether tinagl1 is only in basement membranes at the basal side of cells, or if it also localizes to the apical side of cells where most cilia project. A deeper understanding of the localization of tinagl1 during development is a logical next step in understanding how this protein functions. Zebrafish provide an excellent model for studying this localization as they display strong phenotypic effects that can be easily imaged. The localization of tinagl1 will be tracked using a tinagl1-GFP fusion construct developed through PCR and insertion into a Tol2 transposon vector. This construct will be injected into early embryos together with transposase mRNA to create mosaic fish showing tinagl1-GFP in selected tissues. Successful germline integration of the tinagl1-GFP DNA will lead to the development of a transgenic line of zebrafish allowing imaging of tinagl1 localization during development.en_US
dc.language.isoen_USen_US
dc.publisherAugusta Universityen_US
dc.rightsCopyright protected. Unauthorized reproduction or use beyond the exceptions granted by the Fair Use clause of U.S. Copyright law may violate federal law.en_US
dc.subjectTinagl1en_US
dc.titleEstablishing a GFP Marker in Zebrafish to Study the Localization of Tinagl1en_US
dc.typeThesisen_US
dc.contributor.departmentDepartment of Biological Sciencesen_US
dc.description.advisorLeMosy, Ellen K.
dc.description.embargo2022-12-10


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