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dc.contributor.authorAmanamba, Udochukwu
dc.date.accessioned2021-03-18T19:09:39Z
dc.date.available2021-03-18T19:09:39Z
dc.date.issued2020-12
dc.identifier.urihttp://hdl.handle.net/10675.2/623913
dc.descriptionRecord is embargoed until 12/14/2022en_US
dc.description.abstractDiabetic retinopathy (DR) is a common complication of diabetes and the main cause of blindness among adults of working age. Previous studies have established that high blood glucose levels (hyperglycemia) promote chronic sub-clinical inflammation which in turn causes retinal tissue injury and development of DR. It has also been shown that increased levels of uric acid, a by-product of the purine metabolism, generates crystals of monosodium urate (MSU) which could contribute to retinal inflammation and to the development of DR. My honors thesis project focused on investigating the molecular basis of inflammation in diabetic retinopathy (DR), specifically how MSU stimulates sterile inflammation in retinal blood vessels cells and in other retinal cells through the induction of the NLRP3-inflammasome. Human retinal endothelial (HuREC) and Human retinal epithelial cells (HuRPE) were treated with clinically relevant doses of MSU (6mg/dL) or high glucose (HG 25mM) or a combination of both. The expression of NLRP3 inflammasome constituents such as IL-1, NLRP3 protein, Toll-like receptor (TLR4), Gasdermin D (GSDMD) and Thioredoxin-interacting protein (TXNIP) were monitored using Western blotting analysis and ELISA assay. Morphometric analysis and ANOVA statistical approaches were employed to analyze the data. The results obtained showed that HuREC are more responsive to MSU alone than HuRPE. However, in all conditions, MSU significantly potentiated the production of inflammatory constituents of the NLRP3 inflammasome. Overall, the results of my studies support MSU as a contributing factor to the pathogenesis of DR. This suggests that uricemia should be monitored in diabetic patients and hypouricemic drugs could be helpful in combating DR and vision loss in diabetic patients.en_US
dc.language.isoen_USen_US
dc.publisherAugusta Universityen_US
dc.rightsCopyright protected. Unauthorized reproduction or use beyond the exceptions granted by the Fair Use clause of U.S. Copyright law may violate federal law.en_US
dc.subjectDiabetic retinopathy; crystals of monosodium urate; Human retinal endothelial cells; Human retinal epithelial cellsen_US
dc.titleBlinding Crystals: Monosodium Urate Crystals and Diabetic Retinopathyen_US
dc.typeThesisen_US
dc.contributor.departmentDepartment of Psychological Sciencesen_US
dc.description.advisorBartoli, Manuela
dc.description.embargo2022-12-14


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