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dc.contributor.authorLinatoc, A John
dc.date.accessioned2021-02-22T20:32:53Z
dc.date.available2021-02-22T20:32:53Z
dc.date.issued1993-02
dc.identifier.urien
dc.identifier.urihttp://hdl.handle.net/10675.2/623873
dc.descriptionThe file you are attempting to access is currently restricted to Augusta University. Please log in with your NetID if off campus.
dc.description.abstractThe goals of periodontal therapy are to encourage healing through regeneration of lost periodontal tissue, restore biologic function, improve esthetics, and provide comfort. Fibroblasts are the principal cell type in the connective tissues of the periodontium and they perform important functions in development, physiology, and healing. Extracellular matrix proteins, such as tenascin, are secreted by fibroblasts and may play an important role in morphogenesis and cel,l differentiation. A better understanding of cellular interactions may facilitate the development of predictable regenerative periodontal procedures. The purpose of this study was to examine tenascin localization in human gingival tissues and cultures of two types of human periodontal fibroblasts. Co-cultures of periodontal fibroblasts and human gingival epithelial cells were also produced and examined for tenascin localization. In addition, the effects of medium and time on tenascin secretion by cultured periodontal fibroblasts were quantitatively analyzed by computer assisted densitometric analysis. The results indicate that tenascin is localized at the interface between the overlying epithelium and the connective tissue papilla. Co-cultures failed to show the same tenascin staining pattern as was found in the gingival tissues. No significant difference in tenascin secretion was found between cultured gingival or periodontal ligament fibroblasts. Medium containing 10% fetal bovine serum (FBS) stimulated a significant increase in tenascin production from 48hrs to 96hrs (p50.0405) as compared to serum free medium (p50.0001) or medium conditioned by human gingiv~l fibroblasts (p50.0031), hamster cheek pouch epithelial cells (p50.0001), and human gingival epithelial cells (ps0.02880). It appears that the cell culturing techniques, tissue explant characteristics, the quality and quantity of soluble serum, and/or inducing factors may have influenced the observed pattern of tenascin secretion.en_US
dc.language.isoen_USen_US
dc.publisherMedical College of Georgiaen_US
dc.rightsCopyright protected. Unauthorized reproduction or use beyond the exceptions granted by the Fair Use clause of U.S. Copyright law may violate federal law.en_US
dc.subjectTenascinen_US
dc.subjectPeriodonatal Fibroblasten_US
dc.subjectGingival Epithelial Cellsen_US
dc.titleThe Effects of Human Gingival Epithelial Cells on Tenascin Production by Human Gingival Fibroblastsen_US
dc.typeThesisen_US
dc.contributor.departmentDepartment of Oral Biologyen_US
dc.description.advisorCaughman, Gretchen
dc.description.committeen/a
dc.description.degreeMaster of Science in Oral Biologyen_US
dc.embargoen
refterms.dateFOA2021-02-22T20:32:54Z


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