The Effects of Myostatin Deficiency on Fracture Healing in a Rodent Model
dc.contributor.author | Kellum, Ethan Lasha | |
dc.date.accessioned | 2021-02-22T20:00:49Z | |
dc.date.available | 2021-02-22T20:00:49Z | |
dc.date.issued | 2008-04 | |
dc.identifier.uri | en | |
dc.identifier.uri | http://hdl.handle.net/10675.2/623870 | |
dc.description | The file you are attempting to access is currently restricted to Augusta University. Please log in with your NetID if off campus. | |
dc.description.abstract | Myostatin (GDF-8) is a negative regulator of skeletal muscle growth; mice lacking myostatin show increased muscle mass and enhanced muscle regeneration. It has previously been shown that myostatin deficiency increases bone strength and biomineralization throughout the skeleton. This study tests the hypothesis that myostatindeficiency enhances fracture healing using a fibula osteotomy model. Adult wild-type mice, mice heterozygous for the myostatin mutation, and mice homozygous for the disrupted myostatin sequence were included for study at two- and four-weeks following the osteotomy procedure. Fracture healing was assessed using radiographic, histological, and biomechanical techniques. The fracture callus of myostatin-deficient animals is significantly larger than that of wild-type mice at two- and four-weeks post-osteotomy, and also shows increased total bone area within the callus at each time point. Mechanical testing demonstrates that the fracture callus of myostatin-deficient mice exhibits significantly greater peak force and energy to fracture (toughness) compared to the callus of normal mice. Myostatindeficient animals also have a significantly greater number of regenerative myofibers surrounding the fracture callus compared to normal mice. Results presented here show that the improved muscle regeneration and osteogenic differentiation that accompany myostatin deficiency also enhance bone repair following injury. These findings suggest that myostatin inhibitors may serve as potential therapeutic agents for accelerating muscle and bone healing in orthopaedic trauma cases where significant damage to both muscle and bone has occurred. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Medical College of Georgia | en_US |
dc.rights | Copyright protected. Unauthorized reproduction or use beyond the exceptions granted by the Fair Use clause of U.S. Copyright law may violate federal law. | en_US |
dc.subject | GDF-8 | en_US |
dc.subject | ActRIIB | en_US |
dc.subject | Muscle Regeneration | en_US |
dc.subject | Muscle-bone Interactions | en_US |
dc.subject | Fracture Cellus | en_US |
dc.subject | Myostatin | en_US |
dc.title | The Effects of Myostatin Deficiency on Fracture Healing in a Rodent Model | en_US |
dc.type | Thesis | en_US |
dc.contributor.department | Department of Cellular Biology & Anatomy | en_US |
dc.description.advisor | Hamrick, Mark W | |
dc.description.committee | n/a | |
dc.description.degree | Master of Science | en_US |
dc.embargo | en | |
refterms.dateFOA | 2021-02-22T20:00:50Z |
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Theses and Dissertations [1565]