• Login
    View Item 
    •   Home
    • Theses and Dissertations
    • Theses and Dissertations
    • View Item
    •   Home
    • Theses and Dissertations
    • Theses and Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of Scholarly CommonsCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsThis CollectionTitleAuthorsIssue DateSubmit DateSubjects

    My Account

    LoginRegister

    About

    AboutCreative CommonsAugusta University LibrariesUSG Copyright Policy

    Statistics

    Display statistics

    Astrocytes Modulate Endothelial Cell Growth, Differentiation, and Fibronectin Expression

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    Jiang_Baoen_ PhD_1993.pdf
    Size:
    2.229Mb
    Format:
    PDF
    Download
    Authors
    Jiang, Baoen
    Issue Date
    1993-08
    URI

    http://hdl.handle.net/10675.2/623858
    
    Metadata
    Show full item record
    Abstract
    The retinal vascular development in the nerve fiber layer occurs by in situ differentiation of vascular precursor cells, but the mechanisms controlling this process are still unknown. We sought to determine the stimuli regulating. the organization of vascular precursor cells into retinal vessels by analyzing the roles of extracellular matrix and astrocytes. Immunolocalization analyses in the developing rat show that rat retinal vessels form as a polygonal network, beginning at the optic disc and extending to the ora. serrata by pll. Factor VIII-positive endothelial cells and laminin- and fibronectin-positive basement membrane codistribute with lumenized vessels. GFAP-positive astrocytic processes associate closely with the formed vessels, but ~re also present between the vessels. In the avascular area just distal to the forming vessels, GFAP-positive astrocytes form a fibronectin-positive polygonal meshwork pattern that resembles the immature vascular network. Laminin is not expressed in this area. In the area of the astrocyte-fibronectin meshwork, Bandierea Griffonia Simplifolia I B4 lectin labeled vascular precursor cells elongate and align along the astrocyte processes. Double label analysis indicates that both astrocytes and vascular . cells produce fibronectin. In situ hybridization shows that fibronectin mRNA expression is heaviest in the meshwork areas, suggesting that fibronectin synthesis is required in the early stage of retinal vasculogenesis. 0~11 culture experiments confirmed a strong influence of astrocytes on retinal microvascular endothelial cell growth, morphological differentiation, and extracellular matrix expression. Immunolocalization studies of cultured cells suggested and Western blot and Northern blot analyses confirmed a significant increase in fibronectin expression, when endothelial cells were grown in conditioned medium (CM) from astrocytes as compared with controls grown in CM from endothelial cells or pericytes. When retinal microvascular endothelial cells were grown in CM from astrocyte I endothelial cell co-culture, their growth was significantly inhibited as compared with controls grown in normal growth medium or CM from endothelial cells, astrocytes, or a mixture of 50% astrocyte CM/50% endothelial cell CM. In addition, endothelial cells grown in CM from astrocyte I endothelial cell co-culture elongated and became aligned in capillary-like structures. Endothelial cells grown in CM from astrocytes, endothelial cells, or a mixture of 50%astrocyte CM/50% endothelial cell CM alone retained normal· cobblestone morphology. CM from astrocytes alone stimulates retinal microvascular endothelial cell growth as compared with CM from endothelial cells, astrocyte I endothelial cell co-culture, or a mixture of 50% astrocyte CM/50% endothelial cell CM. These data indicate that rat retinal vascular development occurs by in situ differentiation of vascular precursor cells in close contact with astrocytes. Moreover, astrocytes modulate retinal microvascular endothelial cell growth, morphological differentiation, and fibronectin expression by release of growth factor-like substances. At least two factors are involved - one that requires astrocyte/ endothelial cell contact for activity and one that does not.
    Affiliation
    Department of Cellular Biology & Anatomy
    Description
    The file you are attempting to access is currently restricted to Augusta University. Please log in with your NetID if off campus.
    Collections
    Theses and Dissertations

    entitlement

     
    DSpace software (copyright © 2002 - 2021)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.