Show simple item record

dc.contributor.authorElliis, Portia
dc.date.accessioned2020-11-10T20:00:50Z
dc.date.available2020-11-10T20:00:50Z
dc.date.issued1997-01
dc.identifier.urien
dc.identifier.urihttp://hdl.handle.net/10675.2/623671
dc.description.abstractOrthodontic bonding materials· are present in the oral cavity, bathed in oral fluids and may be in contact with hard and soft tissues for extended periods of time. Therefore, it is important to be aware of the potential toxicity than can result from the components that may leach out of the materials over time. The purpose of this study was to determine the effects of orthodontic bonding materials and sealants (Phase II w/ fluoride--F, Phase II w/o fluoride--NF, Phase II Dual Cure w/light--DCL, Phase II Dual Cure w/o light-- DCN, Light Bond Sealant--LC, and Phase II Sealant--CC) on cell metabolism. Sample disks were aseptically fabricated according to the manufacturer's recommendations. Eluates of materials were prepared by daily transfer of the discs to fresh culture medium (DMEM + 5% ~S) over an 8-day period. Oral epithelial cells were plated in 96 well plates, and after 24 .h of incubation at 37°C and 5% C02, the cells were fed eluatecontaining medium. After an additional 24 h incubation, viable cell numbers were . determined using the MTS assay. DNA and RNA synthesis were determined by labeling ·the cells with eH] thymidine and uridine, respectively. Medium-containing eluates and radioisotopes were added to pre-plated cells as described previously. The raw data from each assay were converted to percent control values. Multifactor AN OVA ( a.=0.05) and least square means analysis were performed on the DNA, RNA and MTS data to evaluate significant effects of days of elution and specimen types. DNA data from day 1 eluates · showed significant stimulation by all specimen types ( 143.3-176.0%) compared to control (p=0.0001), whereas RNA synthesis assay showed significant inhibition by all materials (28.7-59.5%, p=O.OOOl). MTS data revealed that only Phase II w/o fluoride, Phase II Dual Cure w/light and Phase II Sealant produced significant reduction in enzyme activity with day 1 eluates (90.7, 95.8 and 96.6%, respectively) compared to control (p=O.OOOl). Significant differences between specilnen types and days were also noted on other days for each assay. These results suggest that orthodontic bonding materials may have both inhibitory and stimulatory effects on various aspects of cell metabolism and these reactions are time dependent. (Wilmer Eames Study Club and MCG Biocompatibility Progratn)en_US
dc.language.isoen_USen_US
dc.publisherAugusta Universityen_US
dc.rightsCopyright protected. Unauthorized reproduction or use beyond the exceptions granted by the Fair Use clause of U.S. Copyright law may violate federal law.en_US
dc.subjectorthodonticsen_US
dc.subjectbonding materialsen_US
dc.subjectcytotoxicityen_US
dc.titleThe Cytotoxic effects of orthodontic bonding materials on cell metabolismen_US
dc.typeThesisen_US
dc.contributor.departmentMedical College of Georgiaen_US
dc.description.advisorN/A, N/A
dc.description.degreeMaster of Scienceen_US
dc.description.committeeN/A, N/A
dc.embargoen
refterms.dateFOA2020-11-10T20:00:50Z


Files in this item

Thumbnail
Name:
Ellis_Portia_MS_1997.pdf
Size:
2.582Mb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record