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dc.contributor.authorBisch, Frederic C.
dc.date.accessioned2020-10-15T22:28:06Z
dc.date.available2020-10-15T22:28:06Z
dc.date.issued1994-08
dc.identifier.urien
dc.identifier.urihttp://hdl.handle.net/10675.2/623600
dc.description.abstractThe objective of this study was to evaluate the effects of cold storage (4°C) on osteoblast viability and interleukin-6 (IL-6) production. Osteoblasts were harvested from murine calvaria utilizing sequential collagenase digestion and the Ficoll-Paque technique. Twelve-well plates were then inoculated with 2 X 104 cells/ml and placed in cold storage for up to 14 days. The cells were then incubated at 37°C for up to 20 days. During the incubation periods supernatants were collected daily and the cells were evaluated for alkaline phosphatase activity. We developed an enzyme immunoassay (EIA) for IL-6 which was sensitive to 50 pg/ml (standard curve r~ .996) with no cross reactivity with other recombinant murine cytokines. The EIA was th~n employed to assay the supernatants for IL-6 production. Storage at 4 oc for up to 48 hours resulted in a pre9ipitous drop in IL-6 production. After 48 hours in cold storage there was no bone cell via~ility.en_US
dc.language.isoen_USen_US
dc.publisherAugusta Universityen_US
dc.rightsCopyright protected. Unauthorized reproduction or use beyond the exceptions granted by the Fair Use clause of U.S. Copyright law may violate federal law.en_US
dc.subjectInterleukin-6en_US
dc.subjectIL-6en_US
dc.subjectosteoblasten_US
dc.titleThe objective of this study was to evaluate the effects of cold storage (4°C) on osteoblast viability and interleukin-6 (IL-6) production. Osteoblasts were harvested from murine calvaria utilizing sequential collagenase digestion and the Ficoll-Paque technique. Twelve-well plates were then inoculated with 2 X 104 cells/ml and placed in cold storage for up to 14 days. The cells were then incubated at 37°C for up to 20 days. During the incubation periods supernatants were collected daily and the cells were evaluated for alkaline phosphatase activity. We developed an enzyme immunoassay (EIA) for IL-6 which was sensitive to 50 pg/ml (standard curve r~ .996) with no cross reactivity with other recombinant murine cytokines. The EIA was th~n employed to assay the supernatants for IL-6 production. Storage at 4 oc for up to 48 hours resulted in a pre9ipitous drop in IL-6 production. After 48 hours in cold storage there was no bone cell via~ility.en_US
dc.typeThesisen_US
dc.contributor.departmentDepartment of Oral Biologyen_US
dc.description.advisorCaughman, Gretchen
dc.description.degreeMaster of Scienceen_US
dc.description.committeeLake, Francis
dc.description.committeeSchuster, George
dc.description.committeeStein, Sideny
dc.embargoen
refterms.dateFOA2020-10-15T22:28:07Z


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