• Login
    View Item 
    •   Home
    • Theses and Dissertations
    • Theses and Dissertations
    • View Item
    •   Home
    • Theses and Dissertations
    • Theses and Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of Scholarly CommonsCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsThis CollectionTitleAuthorsIssue DateSubmit DateSubjects

    My Account

    LoginRegister

    About

    AboutCreative CommonsAugusta University LibrariesUSG Copyright Policy

    Statistics

    Display statistics

    The objective of this study was to evaluate the effects of cold storage (4°C) on osteoblast viability and interleukin-6 (IL-6) production. Osteoblasts were harvested from murine calvaria utilizing sequential collagenase digestion and the Ficoll-Paque technique. Twelve-well plates were then inoculated with 2 X 104 cells/ml and placed in cold storage for up to 14 days. The cells were then incubated at 37°C for up to 20 days. During the incubation periods supernatants were collected daily and the cells were evaluated for alkaline phosphatase activity. We developed an enzyme immunoassay (EIA) for IL-6 which was sensitive to 50 pg/ml (standard curve r~ .996) with no cross reactivity with other recombinant murine cytokines. The EIA was th~n employed to assay the supernatants for IL-6 production. Storage at 4 oc for up to 48 hours resulted in a pre9ipitous drop in IL-6 production. After 48 hours in cold storage there was no bone cell via~ility.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    Bisch_Frederick_MS_1994.pdf
    Size:
    2.160Mb
    Format:
    PDF
    Download
    Authors
    Bisch, Frederic C.
    Issue Date
    1994-08
    URI

    http://hdl.handle.net/10675.2/623600
    
    Metadata
    Show full item record
    Abstract
    The objective of this study was to evaluate the effects of cold storage (4°C) on osteoblast viability and interleukin-6 (IL-6) production. Osteoblasts were harvested from murine calvaria utilizing sequential collagenase digestion and the Ficoll-Paque technique. Twelve-well plates were then inoculated with 2 X 104 cells/ml and placed in cold storage for up to 14 days. The cells were then incubated at 37°C for up to 20 days. During the incubation periods supernatants were collected daily and the cells were evaluated for alkaline phosphatase activity. We developed an enzyme immunoassay (EIA) for IL-6 which was sensitive to 50 pg/ml (standard curve r~ .996) with no cross reactivity with other recombinant murine cytokines. The EIA was th~n employed to assay the supernatants for IL-6 production. Storage at 4 oc for up to 48 hours resulted in a pre9ipitous drop in IL-6 production. After 48 hours in cold storage there was no bone cell via~ility.
    Affiliation
    Department of Oral Biology
    Collections
    Theses and Dissertations

    entitlement

     
    DSpace software (copyright © 2002 - 2021)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.