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dc.contributor.authorSaj, Dalia
dc.date.accessioned2020-06-16T19:39:25Z
dc.date.available2020-06-16T19:39:25Z
dc.date.issued2020-05
dc.identifier.urihttp://hdl.handle.net/10675.2/623399
dc.descriptionThis file is restricted to Augusta University. Please log in using your JagNet ID and password to access.en_US
dc.description.abstractIncreasing obesity rates have put the American population at higher risk for developing obesity-related medical conditions such as hypertension, heart disease, and diabetes. The hydroxycarboxylic acid (HCA) receptor family is a family of G protein-coupled receptors (GPCRs) that are expressed in adipose tissue and function as metabolic sensors, making them potential pharmaceutical targets in the treatment of obesity and other metabolic disorders. The HCA receptor family consists of the HCA1, HCA2, and HCA3 receptors, which are activated by hydroxycarboxylic acids such as lactate and 3-hydroxybutyric acid. We utilized bioluminescence resonance energy transfer (BRET) to study agonist-induced coupling of luciferase-tagged HCA receptors to Venus fluorescent protein-tagged G protein heterotrimers or arrestins. Our results indicate that the three HCA receptors couple to the Gαi/o subfamily of G proteins. The data additionally confirms a lack of coupling to the other G protein subfamilies (Gαs, Gαq, and Gα12/13), and lacks evidence of arrestin recruitment to HCA receptors. Overall, our study highlights the use of BRET as a powerful tool for analysis of GPCR signaling and demonstrates its possible use for future studies to determine the potency of potential drugs targeting HCA receptors as a therapy for health-related problems such as obesity.en_US
dc.description.sponsorshipAugusta University CURS Student Research Granten_US
dc.description.sponsorshipNational Institutes of Healthen_US
dc.language.isoen_USen_US
dc.publisherAugusta Universityen_US
dc.rightsCopyright protected. Unauthorized reproduction or use beyond the exceptions granted by the Fair Use clause of U.S. Copyright law may violate federal law.en_US
dc.titleProfiling the HCA Receptor Family through BRET Analysis of GPCR-G-Protein and GPCR-Arrestin Interactionsen_US
dc.typeThesisen_US
dc.contributor.departmentDepartment of Biological Sciences; Department of Pharmacology & Toxicologyen_US
dc.description.fundingNIH grant GM130142en_US
dc.description.advisorAngela Spencer, Nevin Lambert
refterms.dateFOA2020-06-16T19:39:26Z


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