• Login
    View Item 
    •   Home
    • Theses and Dissertations
    • Theses and Dissertations
    • View Item
    •   Home
    • Theses and Dissertations
    • Theses and Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of Scholarly CommonsCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsThis CollectionTitleAuthorsIssue DateSubmit DateSubjects

    My Account

    LoginRegister

    About

    AboutCreative CommonsAugusta University LibrariesUSG Copyright Policy

    Statistics

    Display statistics

    The effects of progesterone on matrix mettalloproteinases in cultured human gingival fibroblasts

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    Lohse_Jennifer_MS_2001.pdf
    Size:
    5.231Mb
    Format:
    PDF
    Download
    Authors
    Lohse, Jennifer E.
    Issue Date
    2001-05
    URI

    http://hdl.handle.net/10675.2/623227
    
    Metadata
    Show full item record
    Abstract
    During pregnancy many wo~en suffer from severe gingivitis, which almost never progresses to periodontitis, and resolves post-partum. Since progesterone is elevated during pregnancy and is known to influence MMPs in some reproductive tissues, we theorized that similar effects might be seen in oral tissue. The purpose of this research was to study gestational gingival changes in vitro, some of which may be due to progesterone modulation of certain MMPs. Primary cultures of non-pregnant female and male human gingival fibroblasts (HGF) cells were grown to confluence in 4% FBS in phenol red-free DMEM. The cells were then pre-treated for 72 hours in serum-free DMEM, without or with 10-6 M medroxyprogesterone acetate (MPA). IL-1 ~ (1 ng/ml) was added to initiate MMP production. After 24 hours, medium was removed and cells harvested for reverse·transcriptase - polymerase chain reaction (RT-PCR). Media samples were tested for collagenase production, utilizing zymograms (agarose gels containing gelatin). Cqmmercial ELISAs quantified production of total MMP-3 and pro-MMP-13. The mRNAs for MMPs-1, -2, -3, - 7, -9, -10, -13 and GAPDH (control) were amplified by RT-PCR in order to determine which were modulated by IL-1 ~ +/- MPA. MPA-treated and untreated cells were compared; cells from male and female sources behaved similarly. High levels_ of MMP activity were detected in untreated cells and control stimulated cells with zymogram gels, and levels decreased with MPA treatment. The RT-PCR showed that with the addition of MPA to HGF cultures, there was almost total cessation of specific MMP gene transcription for MMPs-1, -2, -3, -7, -10 and _-13 in response to IL-1 ~- The EL.ISAs confirmed significantly decreased MMP-3 and -13 protein levels (p<0.05). We conclude that MPA has the ability to significantly decrease production and activity of MMPs in response to IL-1 B, which could help to explain why pregnancy gingivitis usually doesn't lead to periodontitis.'
    Affiliation
    School of Graduate Studies
    Collections
    Theses and Dissertations

    entitlement

     
    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.