• Effect of NF-κB Deletion on Bone Marrow Macrophage Respiratory Burst Ability

      Soni, Karan; Bradford, Jennifer; Biological Sciences; Bradford, Jennifer; Augusta University (1/31/2020)
      The nuclear factor-kappaB (NF-κB) signaling pathway is very important in normal immune system function and is also often aberrantly regulated in many different types of cancers. As many cancers are characterized by elevated numbers of infiltrating monocytes/macrophages, we have developed an animal model that lacks canonical NF-κB signaling in bone-marrow derived macrophages (BMDMs). As BMDMs can infiltrate solid cancers, the aim of this particular study was to assess the functionality of phagocyte oxidase ability in NF-κB deficient BMDMs. A respiratory burst assay involves stimulating the phagocyte oxidase enzyme in macrophages to release reactive oxygen species (ROS) so that they can degrade and combat invading pathogens as well as cancer cells. Based on our recent experiments that showed BMDMs lacking p65 had poor phagocytosis ability and low nitrite production, we hypothesize that BMDMs lacking NF-κB signaling will have a decreased respiratory burst response compared to control BMDMs.
    • Prehistoric Dinosaurs: An Exploration of Fact vs. Fiction Through the Creation of Comparative Sculptural Forms

      Havens, Krista; Onofrio, Jennifer; Crowther, Thomas; Art and Design; Onofrio, Jennifer; Crowther, Thomas; Augusta University (1/27/2020)
      Prehistoric Dinosaurs: An Exploration of Fact vs. Fiction Through the Creation of Comparative Sculptural Forms, is an art exhibition which displays the differences between how dinosaurs look in film and media verses how they are proposed to have looked based on scientific findings. The impetus for this project was to create a kid friendly educational tool, comprised of three hand-sculpted dinosaurs and three resin cast dinosaurs. The species of dinosaurs created were Carnotaurus, Velociraptor, and Dilophosaurus, some of the most commonly portrayed dinosaurs in the film industry. Each scientifically accurate dinosaur sculpture will be placed next to its film and media representation counterpart, to allow for the viewer to compare and contrast the differences in the physical appearances.
    • Competitive Balance in Women's Collegiate Golf

      Jones, Austin; Medcalfe, Simon; Hull College of Business; Medcalfe, Simon; Augusta University (1/31/2020)
      Since the implementation of title IX in 1972, there has been in increase in the organization and participation of women's collegiate sports teams. In 1982, which is when Women started competing in NCAA golf, participation was numbered at 739 and by 2008, there were a total of 2047 participants. This paper shows how the increase in participation among division 1 women's golf teams has affected the competitive balance in women's collegiate golf. The method of assessing this effect is to compare all the participating scores in past NCAA championships against the increase in participation over time. It is hypothesized that as participation increased, the scores have trended lower and therefore made women's golf more competitive. The division 1 men's golf team is used as a control to see that the effects are unique to the women's team.
    • Case Competition

      TBD; TBA; TBA; Augusta University (11/20/2019)
      The third Augusta University Case Competition, sponsored by the Hull College of Business, is a competition for student pairs to analyze a given business situation and advise the business's key decision-makers of the merits of their recommended course of action through a written memo. Topics include business processes, internal controls, and ethics. The top five student teams will proceed to a poster display and an in-person presentation to a panel of judges made up of local professionals in the accounting and finance fields. The top team will be named March 6, 2020, and (as discussed with Dr. Patel) the information will be provided for inclusion in the PKP Conference booklet and schedule.
    • Fast-Track Extubation in Infancy and Early Childhood Following Heart Surgery: outcome analysis and predictors of failure

      Geister, Emma; Esquivel, Raquel; Crethers, Danielle; Weatherholt, Danalynn; Sanchez, Maria Gabriela; Munoz, Gustavo; Polimenakos, Anastasios C.; Department of Cellular Biology and Anatomy; Department of Surgery; Polimenakos, Anastasios C.; et al. (1/30/2020)
      Early extubation (EE) has become a critical determinant in perioperative management following congenital heart surgery (CHS) during early childhood. Fast track (FT) strategies and EE, when feasible, can have beneficial effect on clinical outcomes. We sought to determine the impact of EE on clinical outcomes, total hospital costs, identify predictors of failure and suggested criteria for new patients. A retrospective chart review of children ?6 years old (n = 64) who underwent CHS between January-December 2017 was performed. EE was defined as successful removal of the endotracheal tube in the operating room or upon arrival in intensive care unit (ICU). Groups were identified as (A):EE/Fast track and (B):no EE. Determinants for EE failure were assessed, and cost analysis pursued.�We found 39 patients were EE compared to 25 that were not. Children who were EE (mean=6.795 days, sd = 4.250) spend significantly less (p�< 0.0001) overall time in the ICU compared to non-EE patients (mean= 19.960 days, sd= 13.081). We also found that the total hospital stay for patients who were EE (mean= 6.976 days, sd= 4.090) was significantly reduced compared to those who were not (mean=21.783 days, sd=13.450) (p�<0.0001). Furthermore, we found that children who were EE had a significant reduction (p�<0.0001, sd= 23,196.203) in total hospital cost than patients who were not EE. Based on our analysis, we concluded that EE is feasible following CHS during early childhood but requires team approach and thoughtful use of FT protocols.

      Duncan, Leslie; Jensen, Caleb; He, Leilei; Lang, Liwei; Teng, Yong; Biological Sciences; Oral Biology and Diagnostic Sciences; Georgia Cancer Center; Teng, Yong; Lang, Liwei; et al. (1/16/2020)
      A promising arsenal of histone deacetylase (HDAC)-targeted treatment has emerged in the past decade, as the abnormal targeting or retention of HDACs to DNA regulatory regions often occurs in many cancers, including head and neck squamous cell carcinoma (HNSCC). However, few has been studied regarding the beneficial role of HDAC inhibition in anti-HNSCC therapy and the underlying molecular mechanisms remain elusive. Epidermal growth factor receptor (EGFR) is commonly expressed at high levels in HNSCC (more than 90%) and serves as a prime target for new anti-HNSCC therapy. Interestingly, Trichostatin A (TSA), one of HDAC inhibitors, not only inhibits EGFR phosphorylation, but also induces repression of EGFR total protein amount in HNSCC cells. We further show that TSA induces EGFR degradation through the ubiquitin-proteasome pathway in HNSCC cells, which is associated with downregulated AKT and ERK1/2 signaling pathways. The study uncovers that EGFR is one of targets of HDAC-based treatment, providing mechanistic insight into the action of HDAC inhibitors. As there is an increasing interest in using HDAC inhibitors for cancer treatment in the clinic, the outcomes from the present study would be significantly beneficial for the development of new rational HDAC-targeted anticancer modalities.
    • Mindfulness Meditation Through a Mobile App

      Huff, Sabrina; Murray, Cleston-Lee; Restrepo, Leigha; Jones, Shelby; Widner, Sabina; Murray, Cleston - Lee; Psychological Sciences; Widner, Sabina; Augusta University (1/28/2020)
      Personal health is an important aspect of the self. In order to change health, behavior must also be changed. Changing behavior is often effortful and many do not adhere to desired changes. The Theory of Planned Behavior (TPB; Ajzen, 1991) suggests that self-efficacy, attitudes, intention, and subjective norms all play a part in behavior change. This present study examined each of these components as it relates to a particular behavior change, that is, mindfulness meditation. We hypothesized that self-efficacy, attitudes, intention, and subjective norms would positively correlate with the number of minutes spent meditating.� Participants were 144 undergraduates who volunteered for this two-part study. Time One included educating individuals on mindfulness meditation, followed with instructions to meditate for the next six days, log meditation minutes using a mobile app (Smiling Mind), and complete a survey adapted by Azjen (2002) measuring self-efficacy, attitudes, intentions, and subjective norms related to meditating. Time Two consisted of collection of meditation data and another survey on the experience of meditating. Preliminary analyses suggested no relationship between any of the TPB components and meditation, which may cast doubt on the reliability of the TPB constructs to predict behavior change.

      Miranda Henderson; Canela, Jenelly; Fischer, Jeff; Reichmuth, Jessica; Biological Sciences; Fischer, Jeff; Reichmuth, Jessica; Augusta University (1/30/2020)
      Microsporidia are spore-forming obligate intracellular parasitic fungi that infect eukaryotic organisms. They are ubiquitous in nature and infections occur worldwide in terrestrial and aquatic hosts. Some species of Microsporidia have been shown to infect the hepatopancreas of shrimp, which may affect their ability to obtain nutrients, stunt their growth, and increase their susceptibility to additional diseases. Microsporidiosis in shrimp has been shown to negatively impact the commercial shrimp industry, resulting in great economic loss specifically to the state of Georgia since this fishery is the largest and most lucrative. This study was conducted to evaluate the prevalence of microsporidia in shrimp from the Satilla River Estuary in Georgia because of man-made cuts that have altered water quality conditions that could affect shrimp health specifically. Shrimp were caught at four collection sites using 6.1m (20ft) otter trawls and cast nets and were transported on ice back to the lab where they were frozen until dissection. Using bright-field light microscopy and a previously established staining technique, microsporidian spores were detected in hepatopancreas� extracts in greater than 30% of the shrimp analyzed.�
    • Investigating Signaling Pathways Involving the HCA Receptor Family

      Saj, Dalia; Spencer, Angela; Okashah, Najeah; Lambert, Nevin; Biological Sciences; Chemistry and Physics; Department of Pharmacology and Toxicology; Spencer, Angela; Augusta University (1/31/2020)
      Increasing obesity rates have put the American population at higher risk for developing obesity-related medical conditions such as hypertension, heart disease, and diabetes. The hydroxycarboxylic acid (HCA) receptor family is a family of G protein-coupled receptors (GPCRs) that are expressed in adipose tissue and function as metabolic sensors, making them potential pharmaceutical targets in the treatment of obesity and other metabolic disorders. The HCA receptor family consists of the HCA1, HCA2, and HCA3�receptors, which are activated by hydroxycarboxylic acids such as lactate and 3-hydroxybutyric acid. We utilized bioluminescence resonance energy transfer (BRET) to study agonist-induced coupling of luciferase-tagged HCA receptors to Venus fluorescent protein-tagged G protein heterotrimers or arrestins. Our results indicate that the three HCA receptors couple to the Gi/o�subfamily of G proteins. The data additionally confirms a lack of coupling to the other G protein subfamilies (Gs,�Gq,�and G12), and lacks evidence of arrestin recruitment to HCA receptors. Overall, our study highlights the use of BRET as a powerful tool for analysis of GPCR signaling and demonstrates its possible use for future studies to determine the potency of potential drugs targeting HCA receptors as a therapy for health-related problems such as obesity.

      Gaw, Victoria; Glenn, Manderrious; Cannon, Jennifer; Biological Sciences; Cannon, Jennifer; Augusta University (1/31/2020)
      Perfluorooctanoic acid (PFOA) is a synthetic chemical belonging to a larger group of fluorotelomers. These compounds have been used in the production of both industrial and consumer products as surfactants and are environmentally persistent pollutants. While the long-term effects of PFOA are largely unknown, there is increasing evidence suggesting it to be an endocrine disruptor. Studies have shown that PFOA binds to and activates peroxisome-proliferator-activated receptor ? (PPAR?), which can regulate the expression of other genes and receptors. Previous experiments in our lab demonstrated that PFOA treatment of MCF-7 breast cancer cells (an ER?-positive cell line) decreased expression of ER? mRNA and protein, and decreased cell viability by ~20% within 48h of treatment compared to DMSO controls. However, these cells were treated in the absence of fetal bovine serum (FBS).� When we repeated these experiments without serum withdrawal, we initially noted a tendency towards increased proliferation in MCF-7 cells treated with 50�M and 100�M PFOA at both 24h and 48h compared to control. To further examine the role of ER? in this PFOA-induced proliferation, we carried out additional experiments in MCF-7 cells along with experiments in another ER?-positive cell line, T47D, as well as an ER?-negative cell line, MDA-MB-23.
    • Establishing a GFP Marker in Zebrafish to Study the Localization of Tinagl1

      Blackburn, Helena; LeMosy, Ellen; Biological Sciences; Department of Cellular Biology and Anatomy; LeMosy, Ellen; Augusta University (1/27/2020)
      Tinagl1 is a secreted protein found in the basement membrane under epithelial cells. The LeMosy lab previously showed that tinagl1 knockdowns resulted in abnormal spinal development and heart orientation during zebrafish development. These data, together with changes in length of motile cilia, suggested that tinagl1 is involved in cilia function during development. The mechanism of this interaction is unknown, and it is unclear whether Tinagl1 is only in basement membranes at the basal side of cells, or if it also localizes to the apical side of cells where most cilia project. A deeper understanding of the localization of Tinagl1 during development is a logical next step in understanding how this protein functions. Zebrafish provide an excellent model for studying this localization as they display strong phenotypic effects that can be easily imaged. The localization of Tinagl1 will be tracked using a Tinagl1-GFP fusion construct developed through PCR and insertion into a Tol2 transposon vector. This construct will be injected into early embryos together with transposase mRNA to create mosaic fish showing Tinagl1-GFP in selected tissues. Successful germline integration of the tinagl1-GFP DNA will lead to the development of a transgenic line of zebrafish allowing imaging of Tinagl1 localization during development.

      Gunby, Kimberly; Sabbatini, Maria E.; College of Science and Mathematics; Biological Sciences; Sabbatini, Maria; Augusta University (1/30/2020)
      My research is aimed at finding the cyclase-associated protein 1 (CAP1) binding domain on human adenylyl cyclase 3 (AC3). Previous studies in our lab show that the interaction between CAP1 and AC3 inhibits migration and invasion of pancreatic cancer cells. The inhibitory mechanism is thought to involve the binding of AC3 and CAP1, causing the inhibition of globular-actin polymerization needed for filopodia formation and cell motility. A better understanding of this interaction will help facilitate the discovery for drugs that inhibit the migration and invasion of pancreatic cancer cells. To locate the binding region, we constructed mutants of WT AC3 plasmid using a Site-Directed Mutagenesis kit. We substituted a highly conserved proline residue at position 307 for an arginine residue (P307R) and a glutamate residue at position 308 for an alanine residue (E308A). The mutations were confirmed by sequencing. We then transfected pancreatic cancer cell line PANC-1 with WT and mutant AC3 plasmids and confirmed the expression using Western-blotting. To test whether the mutated AC3 could still interact with CAP1, we performed co-immunoprecipitation. We found that the residues proline and arginine in AC3 are not required for the interaction with CAP1. Further substitutions of other conserved residues are underway.
    • Diagnosis of Mental Illness in the Narrator of Charlotte Gilman's "The Yellow Wallpaper" Using the DSM-5

      Fang, Wayne; Sadenwasser, Tim; Biological Sciences; English and Foreign Languages; Sadenwasser, Tim; Augusta University (12/10/2019)
      Charlotte Gilman's "The Yellow Wallpaper" explores mental illness, freedom, and the faults of the rest cure by exploring the life of a wife who has been diagnosed with neurasthenia. With this story Gilman describes an increasingly common practice during her time, and how problematic it was for the individuals who were diagnosed. Through the wife's narration, Gilman shows how many women felt trapped since they were forced to undertake the rest cure due to one-sided relationship dynamics. Using this narrative of the wife's deteriorating mental health, Gilman argues for equality in relationships as well as better treatments for mental health. In this presentation, I will use the Diagnostic and Statistics Manual of Mental Disorders (DSM-5) as well as other scholarly sources to diagnosis the wife's mental illness. To do this, I will take the wife's narration and compare it to diagnostic criteria as presented in the DSM-5. By examining the narrator's thoughts and actions I will be able to examine the progression of her mental illness. Examining the wife's mental health can show how many women of her may have felt trapped. This in turn can explain how many women faced unequal power dynamics in their marriages.
    • The Perception of "The Invisible Empire of The Ku Klux Klan" as a Benevolent Secret Society from 1915 to 1965

      Typhair, Dillon; McClelland-Nugent, Ruth; History, Anthropology, & Philosophy; McClelland-Nugent, Ruth; Augusta University (1/31/2020)
      This paper looks at the history of Americans' changing attitudes toward the Ku Klux Klan. It contributes to the scholarships on Civil War history and domestic terrorism through the case of the KKK. The journalist Edward Pollard's book, The Lost Cause: a new Southern history of the War of the Confederates (1867), influenced generations of Americans both South and North by writing a revisionist history of the Civil War painting confederates as rebels who should still fight to maintain white supremacy. This belief in this "lost cause" led many Americans, in the South especially, to support and have positive attitudes toward the KKK. However even as the Klan claimed to support the ideals of the lost cause, their actions often undermined their claims of benevolence and of the upholding of Southern value. The Klan especially after its revival post-WWI terrorized through violent acts anyone they deemed not "pure American." Today, it is unlikely the Klan will ever be regarded as positively as it once was even if similar hate groups still plague our society.
    • The Application of Low-Cost, Close-Range Photogrammetry in Dentistry

      Patel, Mohit; Mettenburg, Don; Rueggeberg, Frederick; College of Science and Mathematics; Restorative sciences, School of Dentistry; Rueggeberg, Frederick; Augusta University (1/31/2020)
      Currently, three-dimensional scanning is required by a number of procedures in dentistry: Orthodontics, Prosthodontics, Endodontics, Oral Surgery, and General Restorative Dentistry. Close-Range Photogrammetry is a technique that produces three-dimensional coordinates of points identified from multiple images of an object taken at different angles. This technique may provide a low-cost alternative to expensive intra-oral scanning systems and structured light based 3D scanners. The objective of this proof-of-concept project was to evaluate the accuracy and precision of virtual 3D models created using a low cost 3D-printable, open source, 3D scanner (OpenScan) connected to an Adreno controller, Agisoft Metashape software, and a budget smartphone used as a camera. A 3D printed set of teeth were scanned using the system and were also manually measured for comparison. A Layer of wax was applied to a region on the teeth on the model, and a second scan was obtained. The volume of wax coating was calculated using software and compared to measurements taken by hand. The two values were found to be quite similar, proving these initial attempts at accurately scanning dental models were viable for further development
    • Roles of Astrocyte-Derived Estrogen in the Brain

      Meyre, Ja; Brann, Darrell; Wang, Jing; Augusta University Honors Thesis Program; Department of Neuroscience & Regenerative Medicine; Department of Neurology; Brann, Darrell; Wang, Jing; Augusta University (1/28/2020)
      The steroid hormone, 17?-estradiol (E2) is an important hormone that regulates many functions in the body. Traditionally, E2 was believed to be produced primarily by the ovaries in females, but a number of studies have shown that brain cells such as neurons and astrocytes can also make significant quantities of E2. The study presented in this thesis examined the role of astrocyte-derived E2 in exerting neuroprotection in the CA1 region of the hippocampus, as well as its ability to regulate two specific pathways implicated in neuroprotection - the LIF and STAT3 pathways. Since the hippocampal CA1 region is known to be highly vulnerable to global cerebral ischemia (GCI), such as occurs after cardiac arrest, we used a mouse GCI model to examine the neuroprotective role of astrocyte-derived E2 in the hippocampal CA1 region. The results of the study indicate that mice that lack the enzyme aromatase in astrocytes and were unable to produce astrocyte-derived E2, have decreased reactive astrocyte activation after GCI, greater neuronal deficits after GCI in both genders, and they have significantly decreased LIF-STAT3 signaling in the hippocampus.