21st Annual PKP Student Research and Fine Arts Conference: Oral Symposia II
Curcumin Conjugates as Potential Therapeutics for Breast CancerBreast cancer, the target of this study, is one of the most salient forms of cancer in the United States. Among U.S. women, 1 in 8 are diagnosed each year. Current treatment for breast cancer includes dichloroacetic acid (DCA), which is a prescribed for cancer therapy and is primarily effective at a specific high dosage, which leads to side effects such as neuropathy. In a search for an alternative solution with lesser negative effects, curcumin was studied. Curcumin is a component of the turmeric and has an array of health properties including the alleviation of gastrointestinal complications and certain pulmonary diseases and the inhibition of cancer growth. However, curcumin�s main drawback lies in its low bioavailability, thus allowing little to be absorbed into the body upon ingestion. The objective of this study is to design the synthesis for the improvement of DCA as well increased bioavailability of curcumin by conjugating the two components with an amino acid linker in between DCA and curcumin. Prior to synthesis of the amino acid-linked hybrid conjugates, the preceding procedures are standardized. Upon conjugation, it is anticipated that the overall bioavailability would increase, and the effective dosage would decrease, resulting in a potentially more effective breast cancer . The final synthesized compounds will then be analyzed and subsequently studied in breast cancer line cells and animal tests.
Design and Synthesis of Selective COX-2 Inhibitors as Potential Anti-inflammatory AgentsInflammation is a common immune response to harmful pathogens or damaged cells. Non-steroidal anti-inflammatory drugs (NSAIDs) are commonly used to treat inflammation as they inhibit the cyclooxygenase enzyme systems (COX). Selectivity for inhibition of the COX-2 pathway is an aim in the development of NSAIDs, as their adverse side effects are associated with the inhibition of the COX-1 pathway. We have designed and synthesized several hybrid conjugates of ibuprofen with various amino acid linkers showing promising anti-inflammatory properties and decreased gastric ulcer formation. The current developing new hybrid conjugates based on the rational drug design process. The details of the project will be discussed at the conference.
Design, synthesis and computational studies of isoniazid hybrid conjugates as potential antimycobacterial agentsTuberculosis (TB) is a bacterial pathogen caused by Mycobacterium tuberculosis, which generally causes pulmonary infection and is extremely pervasive within the lungs and between subjects. Pyrazinamide (PZA) and isoniazid are first-line anti-tuberculosis prodrug often used in combinational therapy with drugs like ethambutol, streptomycin and/or rifampicin. With prolonged administration of the recommended dose, harmful side effects have been reported: hepatitis, acute hypertension, thrombocytopenia, and gastrointestinal discomfort. To overcome the problem of toxicity and drug resistance, combination therapy has been used which utilizes the simultaneous administration of two or more antibiotics with independent modes of action and different biochemical targets in the bacteria. Recently, the concept of hybrid molecules has been introduced in anticipation that molecules of this type may overcome drug resistance. This multiple target strategy led to the discovery of various bio-effective hybrid molecules. We have synthesized several pyrazinoic acid hybrid conjugates with isoniazid via amino acid linkers with retention of the chiral integrity of the desired products. All the synthesized compounds were characterized by spectral studies. The details of the studies will be discussed at the conference.
CBD analysis in Oils and FoodsCannabidiol (CBD) has become a very prominent topic in the medical community and popular marketplace because of its widespread consumer use. Tetrahydrocannabinol (THC) and other similar molecules can be present in commercial CBD products, so testing is necessary to determine purity of the CBD. Existing methods of analysis for CBD oils are only known on GC-FID (gas chromatography - flame ionization detector) and these methods are not optimal for the wide variety of commercial CBD products available. Thus, I have optimized using a GC-MS method, based on a published GC-FID method, that can be applied to a wide variety of foods, gummies, and other items that may contain CBD and similar molecules. I have optimized the method by varying column temperature, ramp rates, and parameters within the mass spectrometer, to find a balance between run times, analyte detection, and resolution for the CBC/CBD/CBN, etc. cannabinoid molecules present in commercial CBD oil samples. I then used the optimized method on a variety of commercial and self-prepared CBD edibles to assess the recovery and degradation of CBD and other similar molecules.