• In Vivo Pilot Study: Effect of Dehydration/Rehydration on Upper Anterior Tooth Color Change

      Britton, Eduardo; Nappi, A; Cao, T; Shepherd, K; Department of General Dentistry, Dental Hygiene, Department of Restorative Sciences (Augusta University Libraries, 2019)
      Isolation of tooth structure during fabrication of a bonded, direct, resin-based restoration is essential to optimizing its potential for long-term clinical success. Failure to protect etched enamel and bonding agents from contamination by saliva results in inadequate and unpredictable interfacial bonding of the restorative composite, potentially leading to marginal discoloration, open margins, recurrent decay, or ultimately to restoration loss or failure. A consequence of tooth isolation during placement of direct, esthetic restorative resins is the dehydration of enamel surfaces that will not be coated with saliva, and will, over time, lose water that has penetrated into the outer enamel layers (will dehydrate). The longer the tooth isolation time, the greater will be the subsequent loss of water from enamel. Presence of this water in enamel helps to provide for a stable tooth color. In teeth, the observed tooth color is the result of internal light penetration and interaction with tissues below the surface. Enamel is a translucent material, passing a great majority of transmitted light to fall on the more opaque and yellow-colored tissue underneath of it: dentin. In the hydrated state, enamel is more translucent than in its dehydrated state. The white opaque appearance of dehydrated enamel can be of great clinical concern, once a rubber dam has been removed, and the treated teeth with newly placed restorations are observed. Usually, because of the opaque, white nature of recently dehydrated enamel, there is an initial mismatch between an esthetic restoration just placed and its surrounding, remaining enamel. Patients are normally forwarded of this consequence, and are advised that a period of time needs to pass before the surrounding enamel becomes rehydrated, and more translucent (less opaque), before its pre-isolated color returns to a natural state. It is hoped that, at that time, the new restoration will perfectly match the color characteristics of the remaining enamel, and the recent replacement will not be visible at all, but will instead optically blend in without notice. However, prior to that time, there are definitely distinct color differences between a recently placed resin restoration and its surrounding tooth structure. To date, little-to-no information is available on the rate at which a clinician or patient can expect isolated enamel to return to its pre-isolated color, and when to expect this esthetic blending to occur.
    • Influence of Porphyromonas gingivalis on Anti-Apoptotic/Autophagic Signaling Pathways in Human Dendritic Cells

      Meghil, Mohamed; Tawfik, Omnia; Elashirty, Mahmoud; Rajendran, Mythilypriya; Arce, Roger; Schoenlein, Patricia V.; Cutler, Christopher; Department of Oral Biology & Diagnostic Sciences, Department of Periodontics, Department of Cellular Biology and Anatomy (Augusta University, 2019)
      The purpose of this study was to investigate the molecular mechanisims of P. gingivalis-mediated disruption of homeostatic apoptosis and autophagy in DCs.
    • Injection of Tumescent Solution into Maxillary Sinuses During LeFort I Osteotomies for Reduction of Intra-operative Blood Loss

      Zastrow, Stephanie; James, Jeffrey; Department of Oral and Maxillofacial Surgery (Augusta University, 2019)
      Orthognathic surgery is a treatment option for many dentofacial deformities that cannot be treated with orthodontics or minor surgeries alone. Repositioning of the maxilla, mandible, chin, or a combination of the three can have a significant effect on a patient’s occlusal function, facial appearance, and self-esteem. Successful, comprehensive treatment usually requires a collaborative approach between an orthodontist and oral and maxillofacial surgeon.
    • Innate Lymphoid Cells in Periodontitis: A Novel Therapeutic Modality

      Ghaly, Mira; Emami, Golnaz; Khodadadi, Hesam; Mozaffari, Mahmood; Baban, Babak; Department of Periodontics, Department of Oral Biology and Diagnostic Sciences (Augusta University, 2019)
      To determine the presence of ILCs in human periodontium which are emerging immune cells with the potential to be targeted, via novel therapies, in the treatment of peridontitis.
    • Investigation of the Clinical Feasibility of Incorporating Dexmedetomidine into the Outpatient Anesthesia Regimen of Oral & Maxillofacial Surgery

      Taylor, D. Craig; Ferguson, Henry W.; Department of Oral and Maxillofacial Surgery (Augusta University, 2019)
      The purpose of this investigation is to evaluate incorporating a Dexmedetomidine infusion into an established IV Sedation regimen in terms of clinical efficiency (Anesthesia time), Patient subjective experience, and physiologic response to the infusion in comparison to a control appointment without the infusion.
    • Maxillary growth in patients with complete unilateral cleft lip and palate treated with Nasoalveolar molding

      Manente, M; Levy-Bercowski, D; Abreu, A; Fortson, W; Deleon, E; Yu, J; Looney, S; Department of Orthodontics; Department of Plastic Surgery; Department of Biostatistics and Data Science (Augusta University Libraries, 2019)
      In patients with cleft lip and/or palate (CLP), the nasolabial defect has a significant esthetic impact on the face and may impair psychosocial development. Nasoalveolar molding (NAM) is a pre-surgical orthopedic technique aimed to improve the alveolar and nasolabial morphology of patients with cleft lip and palate. This technique is used to facilitate and improve the future surgical correction in cleft lip and palate patients. Influences such as differences in patient age and gingivoperiosteoplasty procedures are among many that have made it difficult for conclusive results to be found and published on the impact of the NAM technique on maxillary growth in patients with complete unilateral cleft lip and palate (CUCLP).
    • A multidisciplinary approach to the management of a maxillary midline diastema: A clinical report

      Romero, Mario F.; Babb, C; Department of Restorative Sciences, Department of General Dentistry (Augusta University, 2019)
      Anterior maxillary spacing has been shown to be one of the most negative influences on self-perceived dental appearance, and a maxillary midline diastema (MMD) is commonly cited by patients as a primary concern during dental consultations. MMD has been defined as a space greater than 0.5 mm between the mesial surfaces of the 2 maxillary central incisors. An MMD greater than 2 mm in the mixed dentition is unlikely to spontaneously close. African Americans are more than twice as likely to have an MMD than whites or Hispanics. In esthetic situations, without a comprehensive smile analysis and proper planning, overtreatment and undesirable effects can occur. Tooth size especially has been emphasized as the primary element of an esthetic smile design. One method of establishing tooth size is tooth biometry as described by Chu. He reported that maxillary anterior tooth widths average 8.5 mm for central incisors, 6.5 mm for lateral incisors, and 7.5 mm for canines and that 80% of the patient population falls within ±0.5 mm of these values. Other important elements of smile analysis include the dental midline, tooth morphology, axial inclinations, and the soft tissue components of gingival health, levels, and harmony. The direct bonding technique is a straightforward, conservative method for diastema closure. However, artistic skills, a knowledge of tooth morphology, and the appropriate selection and use of composite resin materials are essential for success. According to Spear and Kokich, “some existing dentitions simply cannot be restored to a more pleasing appearance without the assistance of several different dental disciplines.” Therefore, complex esthetic dilemmas may require more than one dental discipline, for example, operative dentistry and orthodontics, to establish a functional, maintainable, and pleasant smile. This article illustrates a clinical situation in which an MMD was addressed by first completing a comprehensive smile analysis, followed by closure using limited orthodontics and direct composite resin restorations.
    • Murine Dendritic Cell Interactions with Minor-fimbriae P. gingivalis

      Yuan, J; Auersvald, c; Elashiry, M; Meghil, M; Elashiry, M; Finger Stadler, A; Arce, R.M.; Department of Periodontics (Augusta University, 2019)
      The objective was to determine DC maturity phenotype of murine bone marrow-derived DCs in response to wild/type (PgWT) and minor-fimbriated DPG3.
    • Phosphorylation of EPS8 Mediates Its Downstream Signaling and Biological Functions

      Shahoumi, Linah; Yeudall, W. Andrew; Department of Oral Biology & Diagnostic Sciences, Georgia Cancer Center (Augusta University, 2019)
      The purpose of this study was to investigate the role of EPS8 phosphorylation in modulating biochemical signaling, cell proliferation and motility in HNSCC.

      Bowerman, Brielle; Rueggeberg, FA; Brenes, C; Department of Restorative Sciences, Department of General Dentistry (Augusta University Libraries, 2019)
      A variety of manufacturing techniques have been used throughout the history of dentistry, in order to fabricate indirect restorations. Formative processes (pouring or pressing items into molds) are used when making conventional dentures, or when pressing ceramics. Recently, subtractive fabrication methods have enabled clinicians to mill a wide variety of ceramic and resin-based blanks directly into final forms, fitting the oral structures with high degrees of precision. Examples of older additive techniques include wax buildups to establish missing tooth structure for fabrication of subsequent cast restorations and the manual layering of powdered porcelains for development of ceramic facings on metallic substrates, or for ceramic veneers themselves. Tremendous advancements have been made in the field of 3D digital printing for many industrially based applications. Advances in research and development have resulted in tabletop 3D printers that produce rapid prototype specimens having very high accuracy and surface feature details. Recently, these advances have resulted in the manufacture and availability of a wide variety of 3D digital printers that dental offices now use to directly fabricate a wide range of restorative appliances (denture bases and teeth, temporary restorations, splints) as well as ancillary devices (impression trays, surgical implant guides, casts, try-in set-ups, and stents). Contemporary dental 3D printing typically involves use of near or true ultraviolet radiation (405 nm & 385 nm, respectively) in order to fabricate the basic desired form from a vat of photo-polymerizable monomers. Subsequent to initial form fabrication, the specimen is alcohol-washed of excess surface monomer, and is then subjected to an additional exposure of strong near/UV light, in order to maximize the polymerization process and provide optimal physical properties, as well as to minimize cytotoxicity resulting from leaching of unreacted, residual monomer within the bulk of the as-printed item.

      Faigen, A; James, J; Stevens, M; Department of Oral and Maxillofacial Surgery (Augusta University, 2019)
      Lifetime implant success, in both esthetic and functional categories is a multifactorial process. Connective tissues, hard and soft, play a major role in the health of an implant platform and its associated restoration. While bone loss around the crestal portion of the implant up to 2.0mm within the first year of use is expected. We desire to determine factors which predict bone loss and overall implant success.. Many of the factors which can be used to account for such bone loss are inappropriate stresses and force distribution, trauma during surgery, micromechanical movement, infection, and multiple other patient factors. Bone loss has not been linked to a single implant type, placement procedure, or specialty, but remains a significant challenge in the long-term success of implants.
    • Proliferative Verrucous Leukoplakia (PVL) Expresses High Levels of Toll-Like Receptor 2 (TLR2)

      Koh, Joon; Kurago, Zoya; Georgia Cancer Center (Augusta University, 2019)
      In the current study, we analyzed samples of human oral mucosal PVL and other epithelial disorders to test the possibility that, if TLR2 is involved in early stages of carcinogenesis, then keratinocytes in early-intermediate stages of PVL may express more TLR2 than keratinocytes in non-dysplastic epithelium.
    • The Role of Oral Microbiota in Bisphosphonate-Induced Osteonecrosis of the Jaw: Rat Tibial Defect Model

      Jernigan, Joshua; Awad, Mohamed E.; Elsayed, Ranya; Elsalanty, Mohammed (Augusta University, 2019)
      This study aims to develop a rat model for post-traumatic osteonecrosis at an extra-oral bone site that simulates the oral micro-environment, specifically the proximal tibia.

      Baxter, John; Brenes, C; Rueggeberg, FA; Departments of General Dentistry; Department of Restorative Sciences (Augusta University, 2019)
      Direct, tissue-borne, full denture restoration of edentulous arches has become a well accepted restoration modality for millions of people. In this methodology, the tissue-bearing surface of a polymeric material replacing the form and structure of lost alveolar bond and overlying mucosa rests directly on the residual ridge tissue. Retention of the denture is attained through capillary adhesion forces acting to wet (cover) both the oral mucosa and the polymeric denture bases that rests upon it. Good wetting by saliva is thus one of the many critical features affecting adhesion of the denture base to the oral mucosa. Measurement of the ability of a fluid to wet a surface is performed using the shape of a fluid droplet on that surface. If the fluid wets that surface, then the droplet will spread out. If the fluid does not wet that surface, it will bead up. Quantitative measurement of fluid flow on a surface is performed using measurement of the “contact angle.” In this method, a controlled volume is dispensed onto a surface, and while viewing the interaction of that fluid in silhouette, the angle formed at the tangent of that drop and the flat surface is determined. The lower the contact angle, the more a fluid wets a surface, and the higher its value, the less wetting is that fluid on a specific surface.
    • Time To Open Repair Of Mandible Fractures And Associated Complications

      James, Jeffrey; Farrell, Thomas; Faigen, Alex; Anderson, Jessica; Department of Oral and Maxillofacial Surgery (Augusta University, 2019)
      The aim of this study is to determine whether a correlation exists between the time from injury to repair of mandible fractures and the development of post operative complications.