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dc.contributor.authorTrotter, Leigh Ann
dc.contributor.authorTrotter, Leigh Ann
dc.date.accessioned2019-09-24T18:31:45Z
dc.date.available2019-09-24T18:31:45Z
dc.date.issued12-Apr
dc.identifier.urihttp://hdl.handle.net/10675.2/622654
dc.descriptionThe file you are attempting to access is currently restricted to Augusta University. Please log in with your NetID if off campus.en
dc.description.abstractBlue light treatment alters cellular signaling and affects intracellular biochemical processes in tissues. PURPOSE: This study determined the ability of blue light to modulate Nrf2 and decrease LPS-induced secretion of pro-inflammatory cytokines from cultured, human monocytic cells. METHODS: Cultured THP-1 human monocytic cells were exposed to LPS and blue light treatment. Western Blot analyses, EMSA, and ELISA were used to evaluate NF?B, Nrf2, HO-1, TNF-?, IL-6 and IL-8 production. RESULTS: Light treatment increased nuclear Nrf2 and increased HO-1. Cells pretreated with light had no detectable NF?B-DNA binding. LPS treatment increased nuclear NF?B, and had little effect on Nrf2. Light pre-treatment significantly decreased the amount of TNF-? by 63% and IL-8 by 55%. CONCLUSIONS: Blue light increases the production of Nrf2 and HO-1, decreases the ability of Nf?B to bind in the nucleus, and leads to a decrease in the secretion of pro-inflammatory proteins in human monocytic cells.en_US
dc.titleThe Effect of Nrf2 on Inflammatory Responses of Human Monocytic Cells After Blue Light Exposureen_US
dc.typeThesisen_US
dc.contributor.departmentDepartment of Oral Biologyen_US
dc.description.advisorMesser, Reginaen_US
dc.description.degreeMaster of Science in Oral Biology
dc.description.majorMasters of Science in Oral Biologyen
dc.description.committeeLewis, Jill; Schafer, Tara; Rueggeberg, Freden_US
refterms.dateFOA


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