The Study of 5-HT1D and 5-HT1F Receptor Interactions with G Proteins via BRET Analysis
dc.contributor.author | Trang, Amy | |
dc.date.accessioned | 2019-06-13T20:18:59Z | |
dc.date.available | 2019-06-13T20:18:59Z | |
dc.date.issued | 2019-05 | |
dc.identifier.uri | http://hdl.handle.net/10675.2/622413 | |
dc.description | This file is restricted to Augusta University. Please log in using your JagNet ID and password to access. | en_US |
dc.description.abstract | G protein-coupled receptors (GPCRs) are receptors involved in signal transduction, a process for converting extracellular signals into internal messages to elicit a cellular response. Signal transduction pathways involve activating various G protein subtypes (Gs, Gi/o, Gq/11 and G12/13) which typically lead to second messenger production. Traditionally, second messenger concentration assays are used to identify GPCR coupling with G protein(s), but they are not efficient in profiling GPCRs since they compare the concentrations from different downstream signals. Instead, novel tools, such as Bioluminescence Resonance Energy Transfer (BRET) and mini G (mG) proteins, can be used to profile GPCRs. BRET is a technique that provides quantitative data when protein-protein interaction occurs and requires the proteins of interest to be fused with either a bioluminescent protein or fluorescent protein. In this study, we used mG proteins representing each G protein subtype to identify 5-hydroxytryptamine (5-HT; serotonin) receptor coupling upon serotonin stimulation. Through BRET assays, we determined that both the 5-HT1D and 5-HT1F receptors couple primarily with the mGsiand mGo classes of mG proteins. This supports previous studies that these receptors couple to Gi/o proteins and suggests that the use of mG proteins in BRET assays is an effective tool for GPCR profiling. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Augusta University | en_US |
dc.rights | Copyright protected. Unauthorized reproduction or use beyond the exceptions granted by the Fair Use clause of U.S. Copyright law may violate federal law. | en_US |
dc.title | The Study of 5-HT1D and 5-HT1F Receptor Interactions with G Proteins via BRET Analysis | en_US |
dc.type | Thesis | en_US |
dc.contributor.department | Department of Biological Sciences | en_US |
refterms.dateFOA | 2020-06-15T14:27:41Z |