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dc.contributor.authorLittle, Lauren
dc.date.accessioned2019-06-13T18:45:41Z
dc.date.available2019-06-13T18:45:41Z
dc.date.issued2019-05
dc.identifier.urihttp://hdl.handle.net/10675.2/622407
dc.descriptionThis file is restricted to Augusta University. Please log in using your JagNet ID and password to access.en_US
dc.description.abstractG protein-coupled receptors (GPCRs) are important mediators in cellular signaling and are common targets of drug action. GPCRs are responsible for the transduction of extracellular signals into intracellular signals, mediated by G proteins of four subtypes: Gs, Gi, Gq, and G12/13. A thorough understanding of a signaling pathway involves determining which G protein is coupled to a signal-activated GPCR. In this project, a technique called Bioluminescence Resonance Energy Transfer (BRET) was used to measure the interaction between an activated GPCR from the serotonin (or hydroxytryptamine, 5-HT) receptor family, and G proteins from each subtype. The cDNA for serotonin receptors 5-HT1E and 5-HT2C was fused with the gene for a luminescent protein called Nanoluciferase (Nluc). Then, the receptor-Nluc DNA along with DNA containing a G protein tagged with a fluorescent protein (Venus) was transfected into mammalian cells for expression. Data from BRET assays suggest that the 5-HT1E receptor couples to the Gi/o subclass of G proteins upon serotonin activation, while the 5-HT2C receptor couples to the Gq subclass of G proteins. Profiling serotonin receptors will deepen our understanding of serotonin receptors, associated diseases, and the drugs that target them.en_US
dc.language.isoen_USen_US
dc.publisherAugusta Universityen_US
dc.rightsCopyright protected. Unauthorized reproduction or use beyond the exceptions granted by the Fair Use clause of U.S. Copyright law may violate federal law.en_US
dc.titleInvestigating the Interaction between G Proteins and the 5-HT1E and 5-HT2C Serotonin Receptors Using BRETen_US
dc.typeThesisen_US
dc.contributor.departmentDepartment of Biological Sciencesen_US
dc.description.advisorSpencer, Angela


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