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dc.contributor.authorBraziel, Nina
dc.date.accessioned2019-04-09T14:23:05Z
dc.date.available2019-04-09T14:23:05Z
dc.date.issued1978-06
dc.identifier.urien
dc.identifier.urihttp://hdl.handle.net/10675.2/622268
dc.description.abstractA set of small nuclear RNA.sp~cies (snRNA) in eukaryotic cells have been described. Their cellular function and method of formation is unknown. SnRNAs are found predominately in the nucleus and have been - shown to consist of 9-14 discrete species (1). An in vitro isolated nuclear system capable of synthesizing this class of RNA would greatly facilitate the study of l) the RNA polymerase(s) responsible for their. synthesis and pos$ible derivation from other classes of nuclear RNA, 2) transport from the nucleus and 3) post transcriptional modifications such as methylation and capping. Mouse erythroleukemia cell nuclei were capable of synthesizing RNA linearly for 70 minutes. A labeled ribonucleoside triphosphate was . ' incorporated into· RNA at a rate of 7 pmols/~gnuclear DNA/50 minutes. . " Maximum synthetic activity required the presence of high concentrations of all four ribonucleoside triphosphates (1 mM), a low incubation temperature (25°C), ~nd 5 x 107 nuclei/ml. The effect of a-amanitin on total RNA synthesis was examined. RNA polymerases A, B, and C were functioning in nuclei isolated from MEL cells in mid-log phase. The RNA synthesized .:!.!!_ vitro sedimented in sucrose gradients at approximately 4 to 20S. The synthesis of two species of rapid turn-over snRNA (C and A) was· detected, one with the same electrophoretic mobility as 5S rRNA and one with that of 4.5S pre-tRNA. Indications of the synthesis qf snRNA species D, E, arid F were observed as was the synth~sis ·of snRNA species· ~~, which unlike D, E; and F can be recovered from both cytoplasmic and nuclear cell fractions .en_US
dc.rightsCopyright protected. Unauthorized reproduction or use beyond the exceptions granted by the Fair Use clause of U.S. Copyright law may violate federal law.en_US
dc.subjectRNAen_US
dc.subjectCytoplasmen_US
dc.subjectCell Nucleousen_US
dc.subjectEukaryotic Cellen_US
dc.subjectDNAen_US
dc.titleSmall Nuclear RNA Sythesis in Isolated Mouse Erythroleukemia Cell Nucleien_US
dc.typeDissertationen_US
dc.contributor.departmentDepartment of Cell and Molecular Biologyen_US
dc.description.advisorN/Aen_US
dc.description.degreeMaster of Scienceen_US
dc.description.committeeN/Aen_US
refterms.dateFOA2019-04-09T14:23:05Z


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