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    • 20th Annual Phi Kappa Phi Student Research and Fine Arts Conference (2019)
    • 20th Annual PKP Student Research and Fine Arts Conference: Posters
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    EXAMINING THE EFFECTS OF AETOKTHONOS HYDRILLICOLA EXTRACT ON OXIDATIVE STRESS IN C6 CELLS

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    Authors
    Ward, Kayla
    Issue Date
    2019-02-13
    URI
    http://hdl.handle.net/10675.2/622117
    
    Metadata
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    Abstract
    Aetokthonos hydrillicola, a species of cyanobacteria, has colonized an invasive species of hydrilla in the lakes of the Southeastern United States. This cyanobacterium is suspected to cause Avian Vacuolar Myclinopathy (AVM). AVM is a neurological disease that affect birds. Bald eagles and American coots have been primarily studied and known to be affected by AVM. Symptoms of AVM consist of brain lesions, loss of basic motor skills, and the disease often leads to death. Extracts of A. hydrillicola are toxic to C6 cells, and this cell line is used as a model to examine the mechanism of toxicity. The aim of this research project is to understand the role of oxidative stress in A. hydrillicola cytotoxicity and determine if antioxidant compounds may protect the cells. Common oxidative stress inhibitors, Gingko biloba extract and selenium, have been tested in different concentrations in order to determine if oxidative stress is present and preventable. These compounds did not prevent toxicity in the C6 cells exposed to the cyanobacterial extracts. The presence of oxidative is currently being further investigated using a 2-7 dichlorofluorescin diacetate (DCFH-DA) assay, which indicates the presence of reactive oxygen species.
    Affiliation
    Department of Biological Sciences
    Description
    Presentation given at the 20th Annual Phi Kappa Phi Student Research and Fine Arts Conference
    Collections
    Department of Biological Sciences: Student Research and Presentations
    20th Annual PKP Student Research and Fine Arts Conference: Posters

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