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dc.contributor.authorPatel, Chandani
dc.contributor.authorPatel, Reeya
dc.date.accessioned2019-02-13T20:07:18Z
dc.date.available2019-02-13T20:07:18Z
dc.date.issued2019-02-13
dc.identifier.urihttp://hdl.handle.net/10675.2/622110
dc.descriptionPresentation given at the 20th Annual Phi Kappa Phi Student Research and Fine Arts Conferenceen
dc.description.abstractMicroRNAs (miRNAs) have been known to play a key role in bone regulation. Some miRNAs have been observed to increase bone formation via osteoblast formation and others seem to be involved in bone resorption via osteoclast formation. In this study, we aim to observe which miRNA of those secreted by cells during a traumatic brain injury (TBI) are involved in bone formation or bone resorption. Our focus miRNAs were: miRNA-151, miRNA-6991, miRNA-27a, miRNA-92, and miRNA-1224. Using mouse bone marrow monocytes (BMCs), we have induced osteoclast formation by feeding media containing macrophage colony stimulating factor (M-CSF) as well as receptor activator of nuclear factor kappa-B ligand (RANK-L). After osteoclastogenesis, it has been observed via tartrate resistant acid phosphatase (TRAP) staining that miRNA-151 and miRNA-6991 have been up-regulated during osteoclast differentiation. Of the ones examined in our study, miRNA-27a, miRNA-92, and miRNA-1224 have shown an increase during osteoblast differentiation. The observations from this study can contribute insight for creating possible therapeutic methods for osteoporosis related diseases.
dc.subjectmiRNAen
dc.subjectTBIen
dc.subjectbone lossen
dc.titleMIRNA AND THEIR EFFECTS ON BONE LOSS IN TRAUMATIC BRAIN INJURY PATIENTSen
dc.typePoster Presentationen
dc.contributor.departmentCollege of Science and Mathematicsen
dc.contributor.departmentDepartment of Orthopedic Surgeryen
dc.contributor.sponsorFulzele, Sadananden
dc.contributor.affiliationAugusta Universityen
html.description.abstractMicroRNAs (miRNAs) have been known to play a key role in bone regulation. Some miRNAs have been observed to increase bone formation via osteoblast formation and others seem to be involved in bone resorption via osteoclast formation. In this study, we aim to observe which miRNA of those secreted by cells during a traumatic brain injury (TBI) are involved in bone formation or bone resorption. Our focus miRNAs were: miRNA-151, miRNA-6991, miRNA-27a, miRNA-92, and miRNA-1224. Using mouse bone marrow monocytes (BMCs), we have induced osteoclast formation by feeding media containing macrophage colony stimulating factor (M-CSF) as well as receptor activator of nuclear factor kappa-B ligand (RANK-L). After osteoclastogenesis, it has been observed via tartrate resistant acid phosphatase (TRAP) staining that miRNA-151 and miRNA-6991 have been up-regulated during osteoclast differentiation. Of the ones examined in our study, miRNA-27a, miRNA-92, and miRNA-1224 have shown an increase during osteoblast differentiation. The observations from this study can contribute insight for creating possible therapeutic methods for osteoporosis related diseases.


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