Investigating the Interaction Between G Proteins and the 5-HT1E and 5-HT2C Serotonin Receptors Using BRET
dc.contributor.author | Little, Lauren | |
dc.date.accessioned | 2019-02-13T20:06:23Z | |
dc.date.available | 2019-02-13T20:06:23Z | |
dc.date.issued | 2/13/2019 | |
dc.identifier.uri | http://hdl.handle.net/10675.2/622096 | |
dc.description | Presentation given at the 20th Annual Phi Kappa Phi Student Research and Fine Arts Conference | en |
dc.description.abstract | G protein-coupled receptors (GPCRs) are important mediators in cellular signaling and are common targets of drug action. GPCRs are responsible for the transduction of extracellular signals into intracellular signals, mediated by G proteins of four types: Gs, Gi, Gq, and G12/13. A thorough understanding of a signaling pathway involves determining which G protein is coupled to a signal-activated GPCR. In this project, a technique called Bioluminescence Resonance Energy Transfer (BRET) was used to measure the interaction between an activated GPCR from the serotonin (or hydroxytryptamine, 5-HT) receptor family, and G proteins from each subtype. The cDNA for serotonin receptors 5-HT1E�and 5-HT2C�was fused with the gene for a luminescent protein called Nanoluciferase (Nluc). Then, the receptor-Nluc DNA along with DNA containing a G protein tagged with a fluorescent protein (Venus) was transfected into mammalian cells for expression. Data from BRET assays suggest that the 5-HT1Ereceptor couples to the Go and Gi subclasses of G proteins upon serotonin activation while the 5-HT2C�receptor couples to the Gq class of G proteins. Profiling serotonin receptors will deepen our understanding of serotonin receptors, associated diseases, and the drugs that target them. | |
dc.subject | Serotonin | en |
dc.subject | GPCRs | en |
dc.subject | BRET | en |
dc.title | Investigating the Interaction Between G Proteins and the 5-HT1E and 5-HT2C Serotonin Receptors Using BRET | en |
dc.type | Oral Presentation | en |
dc.contributor.department | Department of Biological Sciences | en |
dc.contributor.department | Department of Chemistry & Physics | en |
cr.funding.source | Augusta University CURS Student Research Grant | en |
dc.contributor.affiliation | Augusta University | en |
dc.contributor.sponsor | Spencer, Angela | en |
html.description.abstract | G protein-coupled receptors (GPCRs) are important mediators in cellular signaling and are common targets of drug action. GPCRs are responsible for the transduction of extracellular signals into intracellular signals, mediated by G proteins of four types: Gs, Gi, Gq, and G12/13. A thorough understanding of a signaling pathway involves determining which G protein is coupled to a signal-activated GPCR. In this project, a technique called Bioluminescence Resonance Energy Transfer (BRET) was used to measure the interaction between an activated GPCR from the serotonin (or hydroxytryptamine, 5-HT) receptor family, and G proteins from each subtype. The cDNA for serotonin receptors 5-HT1E�and 5-HT2C�was fused with the gene for a luminescent protein called Nanoluciferase (Nluc). Then, the receptor-Nluc DNA along with DNA containing a G protein tagged with a fluorescent protein (Venus) was transfected into mammalian cells for expression. Data from BRET assays suggest that the 5-HT1Ereceptor couples to the Go and Gi subclasses of G proteins upon serotonin activation while the 5-HT2C�receptor couples to the Gq class of G proteins. Profiling serotonin receptors will deepen our understanding of serotonin receptors, associated diseases, and the drugs that target them. |