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    • 20th Annual PKP Student Research and Fine Arts Conference: Oral Symposia III
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    Investigating the Interaction Between G Proteins and the 5-HT1E and 5-HT2C Serotonin Receptors Using BRET

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    Authors
    Little, Lauren
    Issue Date
    2019-02-13
    URI
    http://hdl.handle.net/10675.2/622096
    
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    Abstract
    G protein-coupled receptors (GPCRs) are important mediators in cellular signaling and are common targets of drug action. GPCRs are responsible for the transduction of extracellular signals into intracellular signals, mediated by G proteins of four types: Gs, Gi, Gq, and G12/13. A thorough understanding of a signaling pathway involves determining which G protein is coupled to a signal-activated GPCR. In this project, a technique called Bioluminescence Resonance Energy Transfer (BRET) was used to measure the interaction between an activated GPCR from the serotonin (or hydroxytryptamine, 5-HT) receptor family, and G proteins from each subtype. The cDNA for serotonin receptors 5-HT1E�and 5-HT2C�was fused with the gene for a luminescent protein called Nanoluciferase (Nluc). Then, the receptor-Nluc DNA along with DNA containing a G protein tagged with a fluorescent protein (Venus) was transfected into mammalian cells for expression. Data from BRET assays suggest that the 5-HT1Ereceptor couples to the Go and Gi subclasses of G proteins upon serotonin activation while the 5-HT2C�receptor couples to the Gq class of G proteins. Profiling serotonin receptors will deepen our understanding of serotonin receptors, associated diseases, and the drugs that target them.
    Affiliation
    Department of Biological Sciences
    Department of Chemistry & Physics
    Description
    Presentation given at the 20th Annual Phi Kappa Phi Student Research and Fine Arts Conference
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    Department of Chemistry and Physics: Student Research and Presentations
    Department of Biological Sciences: Student Research and Presentations
    20th Annual PKP Student Research and Fine Arts Conference: Oral Symposia III

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