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dc.contributor.authorDoughty, Deanna
dc.date.accessioned2019-02-11T13:37:52Zen
dc.date.available2019-02-11T13:37:52Zen
dc.date.issued2018-12en
dc.identifier.urihttp://hdl.handle.net/10675.2/622072en
dc.descriptionRecord is embargoed until 2/11/2024
dc.description.abstractGlioblastoma (GBM) is the most aggressive and common adult brain tumor subtype, with the majority of patients surviving less than one year. The GBM microenvironment is composed of tumor cells as well as non-cancerous cells, such as microglia, a component of the immune system in the brain. To better understand the role of microglia in GBM, we have optimized in vitroculture conditions for primary microglia. Growing microglia in culture is challenging, but this technique is needed for planned future experiments. Microglia were isolated from mouse neuronal tissue by magnetic bead antibody cell separation using the cellular marker CX3CR1. Isolated microglia were then cultured in various culture conditions, and cellular morphology by light microscopy was used to determine cell health, viability, and activation status. It was determined that the primary microglia grow best in neurobasal media in wells coated with poly-D lysine. Future studies aim to isolate a larger number of cells to allow forco-culture of the inactivated microglia with GBM cells. These results will allow us to better understand the role that microglia play in GBM progression.
dc.language.isoenen
dc.publisherAugusta Universityen
dc.rightsCopyright protected. Unauthorized reproduction or use beyond the exceptions granted by the Fair Use clause of U.S. Copyright law may violate federal law.en
dc.titleOptimizing Isolation and Culture of Primary Microgliaen
dc.typeThesisen
dc.contributor.departmentDepartment of Biological Sciencesen
dc.description.advisorBradford, Jenniferen
dc.contributor.sponsorAmerican Cancer Society -- Institutional Research Granten
dc.contributor.sponsorAugusta University PILOT Awarden
refterms.dateFOA2020-06-15T16:00:09Z
dc.description.embargo2/11/2024


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