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    • 19th Annual PKP Student Research and Fine Arts Conference: Posters
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    CHARACTERIZATION OF NF-¿B DEFICIENT BONE-MARROW MACROPHAGES

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    Authors
    Pepper, Anthony
    Fischer, Jeffrey
    Issue Date
    2018-02-12
    URI
    http://hdl.handle.net/10675.2/621733
    
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    Abstract
    The aim of this study was tocharacterize bone-marrow derived macrophages (BMDMs) that lack canonical nuclear factor-kappaB (NF-?B) signaling. The macrophages for the study were obtained by harvesting the bone-marrow fromp65LysMCre (KO) mice and LysMCrecontrolmice.To determine NF-?B deletion efficiency, p65 (a transcription factor in the canonical pathway) protein levels were evaluated by fluorescent microscopyin bothKOand control BMDMs that had been stimulated withlipopolysaccharide(LPS).The induction ofiNOSwasmonitoredin KO and control BMDMswhenactivated by NF-?B stimulatorsIFN-?andLPS.The regulation of iNOSwas assessedby comparing macrophages that had been treated withLPS, IFN-?, or both to a control treatment under fluorescent microscopy. In addition to staining, a nitric oxide assay was employed to help determine the extent of iNOS activity.The macrophages were also visualized under light microcopyby comparing macrophagesthat were stimulated with LPS andIFN-?tounstimulated cellsusing fluorescence microscopy.Currently,a caspase assay is in progress to help further evaluate the effects of p65 losswithin macrophages.
    Affiliation
    Department of Biological Sciences
    Description
    Presentation given at the 19th Annual Phi Kappa Phi Student Research and Fine Arts Conference
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    19th Annual PKP Student Research and Fine Arts Conference: Posters

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