• High glucose treated cells may lead to cellular senescence effecting function of bladder

      Vincent, Julie; Klee, Nicole; Webb, Clinton; Department of Physiology; College of Education; Klee, Nicole; Department of Physiology; Webb, Clinton; Augusta University (2018-02-12)
      Introduction: Diabetic bladder dysfunctioneffects 30-50% of all diabetespatientsand is characterized by symptoms of overactive and underactive bladder, which greatly effects quality of life.Diabetes is correlated with increased cellular senescence. Senescence is a physiologic phenomenon; however, chronic high levels can lead to tissue dysfunction. Multiple in vitrostudies have shown that high glucose exposure results in an increase in cellular senescent cells.The smooth muscle layer of the bladderis responsible for contraction and relaxation of the bladder; therefore, we hypothesize that primary bladder smooth muscle cells exposed to a high glucose environment will result in an increased number of cellular senescent cells.Methods:Rat primary BSMcells were incubated in normal glucose (4mM), high glucose (22mM), high mannitol (22mM), and bleomycin(+ control). Abeta-galactoside assay was utilized to visualize the presence of senescent cells.Results: Cells treated with high glucose exhibited increased cellular senescent cellscompared to both normal and high mannitol control. Conclusion: We conclude that high glucose exposure increases cellular senescence in primary bladder smooth muscle cells. An increased amount of cellular senescence within the smooth muscle layer of the bladder could contribute to bladder dysfunction as seen with diabetes.