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    • 18th Annual PKP Student Research and Fine Arts Conference: Posters
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    The Exploration of Apoptotic Pathways in MCF-7 Cells in Response to PFOA

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    Authors
    Cannon, Jennifer D.
    Smith, April
    Peloquin, Rachel
    Gillen, Laura
    Urizar, David O.
    Issue Date
    2017-03
    URI
    http://hdl.handle.net/10675.2/621340
    
    Metadata
    Show full item record
    Abstract
    Perfluorooctanoic acid (PFOA) is a widely-used fluoropolymer that has been identified as potentially harmful in high concentrations. Previous experiments conducted in MCF-7 breast cancer cells have shown that 50μM and 100μM PFOA decreases estrogen receptor alpha (ERα) mRNA and protein, peroxisome-proliferator-activated receptor gamma (PPAR γ) mRNA, and causes a 25% decrease in cell viability within 48h of treatment. It is hypothesized that the decreased levels of ER and PPAR leads to apoptosis in these cells. Experiments are currently underway using the Caspase-Glo® 3/7 Assay (Promega) to determine if this is the mechanism by which PFOA decreases viability in MCF-7 cells. Furthermore, a Qiagen® RT2 Profiler 96-Well PCR Apoptosis Array is being performed to determine the effect that PFOA has on 84 apoptotic genes and begin to provide some insight into the mechanisms of apoptosis in MCF-7 cells. It is anticipated that anti-apoptotic genes such as Bcl-2, known to be regulated by ER , will be decreased in PFOA-treated MCF-7 cells.
    Affiliation
    Department of Biological Sciences
    Description
    Poster presented at the 18th Annual Phi Kappa Phi Student Research and Fine Arts Conference
    Collections
    18th Annual PKP Student Research and Fine Arts Conference: Posters
    Department of Biological Sciences: Student Research and Presentations

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