• Login
    View Item 
    •   Home
    • Conferences, Workshops, Lecture Series, and Symposiums
    • Phi Kappa Phi Student Research and Fine Arts Conference
    • 18th Annual Phi Kappa Phi Student Research and Fine Arts Conference (2017)
    • 18th Annual PKP Student Research and Fine Arts Conference: Oral Symposia I
    • View Item
    •   Home
    • Conferences, Workshops, Lecture Series, and Symposiums
    • Phi Kappa Phi Student Research and Fine Arts Conference
    • 18th Annual Phi Kappa Phi Student Research and Fine Arts Conference (2017)
    • 18th Annual PKP Student Research and Fine Arts Conference: Oral Symposia I
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of Scholarly CommonsCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsThis CollectionTitleAuthorsIssue DateSubmit DateSubjects

    My Account

    LoginRegister

    About

    AboutCreative CommonsAugusta University LibrariesUSG Copyright Policy

    Statistics

    Display statistics

    Effectors Implicated in the Adenylyl Cyclase 1 Inhibitory Effect on Cell Migration in Pancreatic Cancer Cells

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    Medepalli_PKP_2017.pdf
    Size:
    2.947Mb
    Format:
    PDF
    Download
    Authors
    Medepalli, Vidya
    Issue Date
    2017-03
    URI
    http://hdl.handle.net/10675.2/621296
    
    Metadata
    Show full item record
    Abstract
    Pancreatic adenocarcinoma is among the most aggressive of all cancers in the United States. Cyclic adenosine monophosphate (cyclic AMP) is a second messenger that regulates the proliferation and migration of pancreatic cancer cells. Cyclic AMP is formed from cytosolic ATP by the enzyme adenylyl cyclase (AC). In the presence of forskolin, a transmembrane AC activator, the proliferation and migration of pancreatic cells have been inhibited. Since the mechanisms underlying the inhibitory effect of activated adenylyl cyclase are little understood, we investigated the downstream mediators implicated in the AC/cyclic AMP pathway. With this purpose, we overexpressed AC1 in the human pancreatic adenocarcinoma (HPAC) cell line, and through the protein kinase A (PKA) inhibitor H-89 and the exchange protein directly activated by cyclic AMP (EPAC) inhibitor ESI-09, we assessed the effector involved upon the treatment with forskolin. In a previous study, we showed that PKA alone mediates the inhibitory effect of forskolin/AC1/cyclic AMP on proliferation of HPAC cells. In the present study, we examined the effectors implicated in the AC1 inhibitory effect on cell migration through utilization of the CytoSelect 24-well cell migration assay kit. Our current experimental data suggests that PKA and EPAC are both likely to be downstream mediators in the effect of forskolin/AC1/cyclic AMP on migration of HPAC cells.
    Affiliation
    Department of Biologic Sciences
    Description
    Presentation given at the 18th Annual Phi Kappa Phi Student Research and Fine Arts Conference
    Collections
    18th Annual PKP Student Research and Fine Arts Conference: Oral Symposia I
    Department of Biological Sciences: Student Research and Presentations

    entitlement

     
    DSpace software (copyright © 2002 - 2021)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.