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  • Arsenal: Volume 1, Issue 2

    Walton, Amberly; Venugopal, Natasha; White, Adam; Layton, James; Alexander, Khadijah; Anosike, Kingsley (Augusta University Libraries, 2017)
  • Studying Gene Expression in Whole Embryos by in situ Hybridization: A Peer-to-Peer Laboratory Guide

    Kalra, Aarushi; Xia, Di; Department of Biological Sciences (Augusta University Libraries, 2017-05-11)
    The extracellular matrix (ECM) plays an important role in cell to cell signaling pathways. Our goal is to provide a full laboratory guide for students to study gene expression in zebrafish embryos by in situ hybridization. Prior to our study, the laboratory had observed disorganized and shortened cilia in cells that are important for cell signaling in the pronephric duct and neural tube floor plate of the zebrafish embryo. Ciliogenesis depends on a master transcriptional regulator, foxj1a, whose mRNA expression can be monitored through in situ hybridization and microscopic imaging. Knockdown morpholino-injected, control mismatched morpholino-injected, and uninjected embryos were fixed to determine if foxj1a transcription is qualitatively affected by ECM gene knockdown. Our results showed that the knockdown embryos portrayed an inconsistent foxj1a signal strength along the length of the pronephric duct, when compared to analysis of control mismatched and wild-type uninjected embryos. We created this manuscript for other students to observe how ECM gene knockdown can affect foxj1a mRNA expression, but also to give them a guide to the tools they would need to explore their own genes of interest, in zebrafish or in many other organisms and tissues.
  • Techniques Used to Establish the First Person Narrator and Perspective in Double Indemnity and Murder, My Sweet

    Walton, Breana; Department of English and Foreign Languages (Augusta University Libraries, 2017-05-11)
    Directed by Billy Wilder and Edward Dmytryk respectively, the films noir Double Indemnity (1944) and Murder, My Sweet (1944) each have a storyline that unfolds from a first person perspective as told by a narrator. The techniques used in the films establish this first person perspective through which the films are understood. Both films include voice over as a technique, which determines who the narrator is and the amount of information withheld or disclosed to the audience. Establishing the visual perspective of the narrator is portrayed through differently for each film. While, Double Indemnity utilizes camera angle, Murder, My Sweet uses camera filters and special effects. Lastly, to achieve the first person narration, the character narrating in each film must be present in every scene or give explanation of events that occur in his absence. The various techniques used in each film function cohesively to establish the narrator and achieve his perspective through which the plot is understood by the audience.
  • Arsenal: Volume 1, Issue 1

    Walton, Amberly; Venugopal, Natasha; White, Adam; Layton, James; Alexander, Khadijah; Anosike, Kingsley (Augusta University Libraries, 2016-10)
  • The Cytotoxic Effects of Novel Persin Analogues on a Breast Cancer Cell Line

    Jones, Keri Leigh; Department of Biological Sciences (Augusta University Libraries, 2016-10)
    Roberts, Gurisik, Biden, Sutherland, and Butt (2007) and Butt et al. (2006) previously found that persin, a compound isolated from avocado leaves, can induce apoptosis, or programmed cell death, in mammary epithelial cells of lactating mice in vivo and in certain human breast cancer cell lines in vitro. It has also been found that at higher doses, persin is cardiotoxic in mice and causes necrosis in mammary glands of lactating mammals (Oelrichs, 1995). Therefore, compounds with reduced mammary gland necrosis and cardiotoxicity but with the apoptotic effects of persin on breast cancer cells could be potential chemotherapeutic agents. Six novel analogues of persin have been synthesized to test their effects on MCF-7 breast cancer cells and MCF-10A normal breast epithelial cells. Cells cultured from each cell line were treated with each analogue at varying concentrations to determine potential cytotoxic doses. Cytotoxicity of the compounds was determined by a commercially available Cell Proliferation Assay. Compounds that were significantly cytotoxic were tested for apoptotic activity using an enzyme-linked immunosorbent assay. Three compounds were found to be cytotoxic to both cell lines, whereas the others had little to no impact on cell viability.
  • The Real "Monster" in Frankenstein

    Urizar, David O.; Department of Biological Sciences (2016-10)
    The story of Frankenstein is typically seen as a battle between Victor Frankenstein and the “monster” of the story. However I argue that that the real “monster” of the story is in fact Victor Frankenstein who is suffering from paranoid schizophrenia and that the “monster” is really just a delusions that Victor uses to cope with the idea that he in fact is the killer of the story. This concept is evident in the fact that no one in the story has ever seen both Victor Frankenstein and the “monster” alive in the same place. The characteristics of the “monster’ also point towards the idea that the “monster” could not possibly exist. Even the way that Victor acts throughout the book point to the idea that he does not really care for the safety of his loved ones. Overall the actions that play out in the story point towards the idea that Victor Frankenstein is the real “monster” of the story.
  • Escape from a Life of Secrets and Emergence of Psychopathy from a Mask of Sanity

    Sivised, Vittika; Department of English and Foreign Languages (2016-10-11)
    This paper explores the progression of psychopathy within the main character of Jim Thompson’s The Killer Inside Me. Lou Ford hides behind a social mask depicting a kindhearted oaf to conceal the psychopathic and violent personality that lies beneath. Throughout the novel, Lou Ford’s psychopathic personality begins to surface as he progresses from mere verbal jabs to murder as he tries to escape from his past and his obligations to his father. These obligations that he has put upon himself keep him from leaving the town; however, as he destroys the chains that bind him to the town by murdering those who represents these chains, his psychopathic personality, which is his real personality, grows in strength, and soon, the truth of his violent nature is known by the rest of the characters. In the end, to truly escape from the town, Lou Ford commits his final act: suicide. This act of suicide frees him from the past and he was able to be who he always was, a psychopath.