• Login
    View Item 
    •   Home
    • Colleges & Programs
    • Medical College of Georgia (MCG)
    • Department of Biochemistry and Molecular Biology
    • Department of Biochemistry and Molecular Biology: Faculty Research and Presentations
    • View Item
    •   Home
    • Colleges & Programs
    • Medical College of Georgia (MCG)
    • Department of Biochemistry and Molecular Biology
    • Department of Biochemistry and Molecular Biology: Faculty Research and Presentations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of Scholarly CommonsCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsThis CollectionTitleAuthorsIssue DateSubmit DateSubjects

    My Account

    LoginRegister

    About

    AboutCreative CommonsAugusta University LibrariesUSG Copyright Policy

    Statistics

    Display statistics

    Amino acid derivatives are substrates or non-transported inhibitors of the amino acid transporter PAT2 (slc36a2)

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    Publisher version
    View Source
    Access full-text PDFOpen Access
    View Source
    Check access options
    Check access options
    Authors
    Ganapathy, Vadivel
    Issue Date
    2011-01
    URI
    http://hdl.handle.net/10675.2/616
    
    Metadata
    Show full item record
    Abstract
    The H+-coupled amino acid transporter PAT2 (SLC36A2) transports the amino acids proline, glycine, alanine and hydroxyproline. A physiological role played by PAT2 in amino acid reabsorption in the renal proximal tubule is demonstrated by mutations in SLC36A2 that lead to an iminoglycinuric phenotype (imino acid and glycine uria) in humans. A number of proline, GABA and tryptophan derivatives were examined to determine if they function either as transported substrates or non-transported inhibitors of PAT2. The compounds were investigated following heterologous expression of rat PAT2 in Xenopus laevis oocytes. PAT2 function was characterised by: radiotracer uptake and competition (cis-inhibition) studies; radiotracer efflux and trans-stimulation; and measurement of substrate-induced positive inward current by two-electrode voltage-clamp. In general, the proline derivatives appeared to be transported substrates and the relative ability to induce current flow was closely related to the inhibitory effects on PAT2-mediated
    Research Highlights: ⠺PAT2 (SLC36A2) transports proline, glycine, alanine and hydroxyproline. ⠺Heterocyclic proline derivatives are transported substrates. ⠺Heterocyclic GABA derivatives are translocated slowly. ⠺Tryptophan derivatives (e.g. α-methyl-
    Abbreviations: 1-ACHC, 1-aminocyclohexanecarboxylic acid
    Keywords: PAT2
    Citation
    Biochim Biophys Acta. 2011 Jan; 1808(1):260-270
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.bbamem.2010.07.032
    Scopus Count
    Collections
    Department of Biochemistry and Molecular Biology: Faculty Research and Presentations

    entitlement

    Related articles

    • Substrate specificity and functional characterisation of the H+/amino acid transporter rat PAT2 (Slc36a2).
    • Authors: Kennedy DJ, Gatfield KM, Winpenny JP, Ganapathy V, Thwaites DT
    • Issue date: 2005 Jan
    • Transport of L-proline by the proton-coupled amino acid transporter PAT2 in differentiated 3T3-L1 cells.
    • Authors: Zebisch K, Brandsch M
    • Issue date: 2013 Feb
    • A novel bifunctionality: PAT1 and PAT2 mediate electrogenic proton/amino acid and electroneutral proton/fatty acid symport.
    • Authors: Foltz M, Boll M, Raschka L, Kottra G, Daniel H
    • Issue date: 2004 Nov
    • Serotonin, L-tryptophan, and tryptamine are effective inhibitors of the amino acid transport system PAT1.
    • Authors: Metzner L, Kottra G, Neubert K, Daniel H, Brandsch M
    • Issue date: 2005 Sep
    • Substrate specificity and transport mode of the proton-dependent amino acid transporter mPAT2.
    • Authors: Foltz M, Oechsler C, Boll M, Kottra G, Daniel H
    • Issue date: 2004 Aug
    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.