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dc.contributor.authorDing, Lingling
dc.contributor.authorKuhne, Wendy W.
dc.contributor.authorHinton, David E.
dc.contributor.authorSong, Jian
dc.contributor.authorDynan, William S.
dc.date.accessioned2012-10-26T16:26:51Z
dc.date.available2012-10-26T16:26:51Z
dc.date.issued2010-10-11en_US
dc.identifier.citationPLoS One. 2010 Oct 11; 5(10):e13287en_US
dc.identifier.issn1932-6203en_US
dc.identifier.pmid20949019en_US
dc.identifier.doi10.1371/journal.pone.0013287en_US
dc.identifier.urihttp://hdl.handle.net/10675.2/601
dc.description.abstractBackground: Small laboratory fish share many anatomical and histological characteristics with other vertebrates, yet can be maintained in large numbers at low cost for lifetime studies. Here we characterize biomarkers associated with normal aging in the Japanese medaka (Oryzias latipes), a species that has been widely used in toxicology studies and has potential utility as a model organism for experimental aging research.
dc.description.abstractPrincipal Findings: The median lifespan of medaka was approximately 22 months under laboratory conditions. We performed quantitative histological analysis of tissues from age-grouped individuals representing young adults (6 months old), mature adults (16 months old), and adults that had survived beyond the median lifespan (24 months). Livers of 24-month old individuals showed extensive morphologic changes, including spongiosis hepatis, steatosis, ballooning degeneration, inflammation, and nuclear pyknosis. There were also phagolysosomes, vacuoles, and residual bodies in parenchymal cells and congestion of sinusoidal vessels. Livers of aged individuals were characterized by increases in lipofuscin deposits and in the number of TUNEL-positive apoptotic cells. Some of these degenerative characteristics were seen, to a lesser extent, in the livers of 16-month old individuals, but not in 6-month old individuals. The basal layer of the dermis showed an age-dependent decline in the number of dividing cells and an increase in senescence-associated β-galactosidase. The hearts of aged individuals were characterized by fibrosis and lipofuscin deposition. There was also a loss of pigmented cells from the retinal epithelium. By contrast, age-associated changes were not apparent in skeletal muscle, the ocular lens, or the brain.
dc.description.abstractSignificance: The results provide a set of markers that can be used to trace the process of normal tissue aging in medaka and to evaluate the effect of environmental stressors.
dc.rightsDing et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.subjectResearch Articleen_US
dc.subjectBiochemistryen_US
dc.subjectGeriatricsen_US
dc.subjectGastroenterology and Hepatology/Hepatologyen_US
dc.subjectPathology/Histopathologyen_US
dc.subject.meshAgingen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBiological Markersen_US
dc.subject.meshEyeen_US
dc.subject.meshIn Situ Nick-End Labelingen_US
dc.subject.meshLiveren_US
dc.subject.meshMuscle, Skeletalen_US
dc.subject.meshMyocardiumen_US
dc.subject.meshOryziasen_US
dc.subject.meshSkinen_US
dc.titleQuantifiable Biomarkers of Normal Aging in the Japanese Medaka Fish (Oryzias latipes)en_US
dc.typeArticleen_US
dc.identifier.pmcidPMC2952620en_US
dc.contributor.corporatenameInstitute of Molecular Medicine and Genetics
refterms.dateFOA2019-04-09T22:34:29Z
html.description.abstractBackground: Small laboratory fish share many anatomical and histological characteristics with other vertebrates, yet can be maintained in large numbers at low cost for lifetime studies. Here we characterize biomarkers associated with normal aging in the Japanese medaka (Oryzias latipes), a species that has been widely used in toxicology studies and has potential utility as a model organism for experimental aging research.
html.description.abstractPrincipal Findings: The median lifespan of medaka was approximately 22 months under laboratory conditions. We performed quantitative histological analysis of tissues from age-grouped individuals representing young adults (6 months old), mature adults (16 months old), and adults that had survived beyond the median lifespan (24 months). Livers of 24-month old individuals showed extensive morphologic changes, including spongiosis hepatis, steatosis, ballooning degeneration, inflammation, and nuclear pyknosis. There were also phagolysosomes, vacuoles, and residual bodies in parenchymal cells and congestion of sinusoidal vessels. Livers of aged individuals were characterized by increases in lipofuscin deposits and in the number of TUNEL-positive apoptotic cells. Some of these degenerative characteristics were seen, to a lesser extent, in the livers of 16-month old individuals, but not in 6-month old individuals. The basal layer of the dermis showed an age-dependent decline in the number of dividing cells and an increase in senescence-associated β-galactosidase. The hearts of aged individuals were characterized by fibrosis and lipofuscin deposition. There was also a loss of pigmented cells from the retinal epithelium. By contrast, age-associated changes were not apparent in skeletal muscle, the ocular lens, or the brain.
html.description.abstractSignificance: The results provide a set of markers that can be used to trace the process of normal tissue aging in medaka and to evaluate the effect of environmental stressors.


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