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EFFECT OF GABAERGIC NEURONS IN SUSCEPTIBLE VERSUS RESILIENT MALE RATS TO PTSDPost-traumatic stress disorder (PTSD) is a psychological disorder that can occur after a traumatic event. Individuals with PTSD exhibit extreme anxiety and learning and memory difficulties. Once exposed, 12-35% develop PTSD with women twice as likely to be affected than men. Our goal is to discover underlying mechanisms to prevent PTSD, as we investigate the relevance of glutamic acid decarboxylase positive (GAD+) neurons on susceptible (SUS) and resilient (RES) male rats. SUS and RES phenotypes were assessed using the highly advanced RISP protocol to reveal susceptibility to a PTSD-like phenotype. The increase of GAD+ cells in the medial prefrontal cortex (mPFC) informs us that more GABAergic neurons are present, which can cause inappropriate recall. We will be examining if there is a difference in the number of GAD+ cells in the RES versus SUS male rats. To investigate, we used cryosectioned brains from SUS or RES rats. The brains were stained using immunohistochemistry to isolate the GAD+ neurons in the mPFC and were counted. The results of this experiment will be determined and examined at a later date closer to our presentation. We expect to see a SUS male rats to have a higher number of GAD+ neurons.