• LAVANDULA ANGUSTIFOLIA USE FOR DYSMENORRHEA IN YOUNG WOMEN: A REVIEW OF THE LITERATURE

      Nelson, Brenda; College of Science and Mathematics; College of Nursing; Langley-Brady, Dawn; Augusta University (2019-02-13)
      Dysmenorrhea effects 20% of women causing missed school and work days and interferes with daily life. Dysmenorrhea is caused by menstrual uterine contractions which may result in pain, nausea, vomiting and headaches. Many women utilize pharmacological symptom management, but experience side effects such as edema, libido reduction and increased symptom severity. Aromatherapy is a holistic non-pharmacological approach to symptom reduction. Aromatherapy is the use of essential oils via inhalation or topical application to relieve pain, stress and more.� The purpose of this project is to review the literature surrounding�Lavandula angustifolia�(lavender) and dysmenorrhea to give a foundation for future research. PubMed, TRIP, and Cochrane Library databases were searched for peer-reviewed journals articles in English and published within the last 10 years with the following keywords: dysmenorrhea, lavender, aromatherapy and human. The literature review resulted in six articles meeting inclusion criteria. These articles established the effectiveness of lavender in reducing dysmenorrhea pain in the first three days of menstruation, through inhalation and abdominal application. Lavender essential oil is also effective in reducing nausea and headaches resulting in an alternative for women experiencing dysmenorrhea. Aromatherapy has fewer risks than pharmacological and surgical approaches to dysmenorrhea management and should be studied further.
    • SYNTHESIS OF COUMARIN-LABELED AMINO ACIDS VIA �CLICK� CHEMISTRY

      Weathers, Angel; Department of Chemistry and Physics; Lebedyeva, Iryna; Augusta University (2019-02-13)
      �Click� Chemistry is a convenient technique often applied during the synthesis of various bioconjugates. Several methods have been developed to administer �click� chemistry. Copper(1)-catalyzed azide-alkyne cycloaddition (CuAAC) reactions usually provide high chemoselectivity under green reaction conditions, and good to great yields. Because of this, CuAAC reactions serve many applications in chemical biology. In copper halide catalyzed reactions copper iodide is frequently used to facilitate transformational changes within the reaction. Copper(1) is combined with salts, metal complexes, or ionic liquids to provide effective catalytic systems for reactions. The use of co-solvent systems such as the one used in this research project, dimethylformamide and water, improves reaction efficiency. The alkyne moiety is an indispensible component of �click� chemistry reactions. Coumarins are the specific class of fluorophores examined in this research project because they are highly sensitive fluorescent laser dyes that have extended spectra range, high emission quantum yields, and better solubility compared to more complex fluorescent tags. Amino acids are often used as building blocks because they are recognized by cell membrane proteins more readily. In this project, a number of amino acids labeled with coumarin tags through CuAAC catalyzed 1,2,3-triazole links have been developed to study their fluorescent properties.
    • That's On You, Not Me

      Miles, Edgar; Department of Communication; O'Meara, Melanie; Augusta University (2019-02-13)
      �That�s On You, Not Me� is a performance piece that was created in response to an assignment prompt for Dr. Melanie O�Meara�s Voice and Movement class in Spring 2018. The assignment was to write a haiku and perform that haiku using at least 12 individual vocal variations. Gender expression and the ways alternative expressions are received in various social contexts are existing themes in my visual art practice, so I decided to continue that exploration in my performance work for Dr. O�Meara�s class. In doing so, my performance addresses the discomfort that people experience when faced with expression that violates their expectations and whose responsibility it is to mitigate that discomfort. I present to the audience twelve individual characters created through vocal and movement variation. My intention is to open conversations about gender norms, societal expectations, the experience of �othering,� and respect for individual expression. The performance itself lasts only about three minutes, but can be followed by a brief talk about the work and a question-and-answer session.
    • Investigating the Interaction Between G Proteins and the 5-HT1E and 5-HT2C Serotonin Receptors Using BRET

      Little, Lauren; Department of Biological Sciences; Department of Chemistry & Physics; Spencer, Angela; Augusta University (2019-02-13)
      G protein-coupled receptors (GPCRs) are important mediators in cellular signaling and are common targets of drug action. GPCRs are responsible for the transduction of extracellular signals into intracellular signals, mediated by G proteins of four types: Gs, Gi, Gq, and G12/13. A thorough understanding of a signaling pathway involves determining which G protein is coupled to a signal-activated GPCR. In this project, a technique called Bioluminescence Resonance Energy Transfer (BRET) was used to measure the interaction between an activated GPCR from the serotonin (or hydroxytryptamine, 5-HT) receptor family, and G proteins from each subtype. The cDNA for serotonin receptors 5-HT1E�and 5-HT2C�was fused with the gene for a luminescent protein called Nanoluciferase (Nluc). Then, the receptor-Nluc DNA along with DNA containing a G protein tagged with a fluorescent protein (Venus) was transfected into mammalian cells for expression. Data from BRET assays suggest that the 5-HT1Ereceptor couples to the Go and Gi subclasses of G proteins upon serotonin activation while the 5-HT2C�receptor couples to the Gq class of G proteins. Profiling serotonin receptors will deepen our understanding of serotonin receptors, associated diseases, and the drugs that target them.
    • MIRNA AND THEIR EFFECTS ON BONE LOSS IN TRAUMATIC BRAIN INJURY PATIENTS

      Patel, Chandani; Patel, Reeya; College of Science and Mathematics; Department of Orthopedic Surgery; Fulzele, Sadanand; Augusta University (2019-02-13)
      MicroRNAs (miRNAs) have been known to play a key role in bone regulation. Some miRNAs have been observed to increase bone formation via osteoblast formation and others seem to be involved in bone resorption via osteoclast formation. In this study, we aim to observe which miRNA of those secreted by cells during a traumatic brain injury (TBI) are involved in bone formation or bone resorption. Our focus miRNAs were: miRNA-151, miRNA-6991, miRNA-27a, miRNA-92, and miRNA-1224. Using mouse bone marrow monocytes (BMCs), we have induced osteoclast formation by feeding media containing macrophage colony stimulating factor (M-CSF) as well as receptor activator of nuclear factor kappa-B ligand (RANK-L). After osteoclastogenesis, it has been observed via tartrate resistant acid phosphatase (TRAP) staining that miRNA-151 and miRNA-6991 have been up-regulated during osteoclast differentiation. Of the ones examined in our study, miRNA-27a, miRNA-92, and miRNA-1224 have shown an increase during osteoblast differentiation. The observations from this study can contribute insight for creating possible therapeutic methods for osteoporosis related diseases.
    • A Comparitive Study of Epilepsy in Galenic, Medieval Persian and Modern Medicine

      Alapatt, Vinaya Ann; Department of Psychological Sciences; Department of History, Anthropology & Philosophy; Turner, Wendy; Augusta University (2019-02-13)
      Epilepsy is an interesting neurological disorder that exists at the crossroads of biology and spirituality. This research examined the transmission of Greek theories of epilepsy from the ninth to the thirteenth century Persian medicine and compared it to the understanding of epilepsy in modern medicine. The influence of Galenic medicine on the clinical understanding of epilepsy in medieval Persian medicine (800-1400) is evident in Ibn Sina's (aka Avicenna) medical manuscripts. Given the complex technological advancements from 13th century to 21st century, substantial progression in the understanding of epilepsy from Avicennian period to modern era was expected to find. However, modern medicine is yet to crack the full codes of this "sacred" disease. Tracing the scientific history of epilepsy reveals that today's identified etiology, symptomatology, and treatments for epilepsy, which hugely benefited from the technological advancements in diagnostic means, are extensions to the medieval understanding of epilepsy. This paper is a comparative study of epilepsy in Galenic, medieval Persian and modern medicine. On a broad scale, this research serves as an example on how ideas connect people through time.
    • Exploring the minimum flow rate limit in electro co-flow

      Overlie, Benjamin; Department of Biological Sciences; Department of Chemistry & Physics; Millan, Josefa Guerrero; Augusta University (2019-02-13)
      Controlled generation of micron and sub-micron sized drops continues to be of strong interest for the scientific community due to the variety of applications in fields like cosmetics, food industry, and drug delivery among others. By flowing two immiscible liquids into a glass-based microfluidic device, we can make emulsion drops with a minimum size of the order of the tip size. Adding an external electric field, similarly to what it is done in the classical electrospray, allows the generation of droplets with sizes below the smallest geometrical characteristic of the device. In this work, we are focused on the region of small flow rates. There is a minimum flow rate below which a cone-jet cannot be formed regardless of the applied voltage. This limit marks the minimum drop size that could be generated. We study pairs of liquids with different viscosities and conductivities using high speed microscopy and current measurements. With these data we will try to understand this limit and the characteristics of the modes observed in this region.
    • CHARACTERIZING THE ROLE OF PANCREATIC STELLATE CELLS IN THE TRANSITION OF CHRONIC PANCREATITIS TO PANCREATIC CANCER

      Godoy, Catalina; Department of Biological Sciences; Department of Pharmacology & Toxicology; Csanyi, Gabor; Sabbatini, Maria; Augusta University (2019-02-13)
      Background- Chronic pancreatitis (CP) and pancreatic cancer are two diseases that share a mutual histological feature known as fibrosis produced by pancreatic stellate cells (PaSCs). In response to pancreatic inflammation, PaSCs are activated from quiescent phenotype into myofibroblast-like cells, which express extracellular matrix components. PaSCs are also known to facilitate the migration and invasion of pancreatic cancer cells, which are accompanied by increased matrix metalloprotease (MMP) production and epithelial-to mesenchymal transition (EMT). NADPH oxidase (Nox) is a family of enzymes that catalyze the transfer of an electron from NAD(P)H to oxygen to generate superoxide or hydrogen peroxide. Because Nox1 is expressed in PaSCs, the objective of this study was to assess the extent to which Nox1 in PaSCs facilitates the migration and invasion of pancreatic cancer cells by regulating the expression of MMP and genes involved in EMT. Results/Discussion-We found that the lack of Nox1 lowers the expression of MMP-9 mRNA and the EMT-induced gene Snail in PaSCs. Further studies need to be done in PaSCs from mice with CP and CP-associated oncogenic KRas-driven pancreatic cancer.
    • Design, Synthesis, and Anti-inflammatory Studies of NSAID Hybrid Conjugates

      Honkanadavar, Hitesh; Department of Chemistry & Physics; Panda, Siva; Augusta University (2019-02-13)
      Non-steroidal anti-inflammatory drugs are one of the most common drugs administered worldwide as highly effective analgesic, antipyretic and anti-inflammatory agents. The drugs function by inhibiting the COX-2 enzyme system which leads to a decrease in inflammation; however, the drugs also inhibit the COX-1 enzyme system which is critical to normal renal function, gastric mucosal integrity, vascular homeostasis, and the autocrine response to circulating hormones which can lead to gastric ulcers and renal dysfunction. Hybrid conjugates of existing non-steroidal anti-inflammatory drugs (NSAIDs) have already been synthesized with Ibuprofen, Acetaminophen, and amino acids to increase potency and decrease toxicity. Computational chemistry studies of these compounds show that the free phenol moiety in acetaminophen plays a greater role in the inhibition of the COX 2 enzyme system than the amine moiety. The previous compounds utilized the phenol moiety to form the product. New hybrid conjugates of Ibuprofen and Acetaminophen via amino acid linkers have been synthesized, leaving the phenol moiety free. The compounds have been characterized by NMR and IR. Biological studies indicate that some of the synthesized compounds are showing improved potency when compared to Ibuprofen alone. Computational chemistry studies and molecular modeling will be used to support the�in-vivo�biological activity.
    • The Study of 5ht-1d and 5ht-1f Receptor Interactions with Mini G Proteins via Bret Analysis

      Trang, Amy; Department of Chemistry & Physics; Department of Pharmacology and Toxicology; Spencer, Angela; Lambert, Nevin; Augusta University (2019-02-13)
      G protein-coupled receptors (GPCRs) are receptors involved in signal transduction, a process for converting extracellular signals into internal messages to elicit a cellular response. Signal transduction pathways involve activating various G protein subtypes (Gs, Gi/o, Gq/11�and G12/13) which typically lead to second messenger production. Traditionally, second messenger concentration assays are used to identify GPCR coupling with G protein(s), but they are not efficient in profiling GPCRs since they compare the concentrations from different downstream signals. Instead, novel tools, such as Bioluminescence Resonance Energy Transfer (BRET) and mini G (mG) proteins, can be used to profile GPCRs. BRET is a technique that provides quantitative data when protein-protein interaction occurs and requires the proteins of interest to be fused with either a bioluminescent protein or fluorescent protein. In this study, we used mG proteins representing each G protein subtype to identify 5-hydroxytryptamine (5-HT; serotonin) receptor coupling upon serotonin stimulation. Through BRET assays, we determined that both the 5-HT1D�and 5-HT1F�receptors couple primarily with the mGsiand mGo�classes of mG proteins. This supports previous studies that these receptors couple to Gi/o�proteins and suggests that the use of mG proteins in BRET assays is an effective tool for GPCR profiling.
    • Unconventional Coupling of 5HT7 receptors to Gs heterotrimers

      Adams, Elizabeth; Department of Pharmacology and Toxicology; Lambert, Nevin; Augusta University (2019-02-13)
      GPCRs play a major role in cell signaling through their interactions with heterotrimeric G proteins. In conventional models of GPCR-G protein coupling, agonist binding promotes a conformational change within the receptor, which then associates with G proteins, facilitating the exchange of GDP for GTP. GTP-bound G proteins dissociate from the receptor and exert their effects on downstream signaling molecules. Previous studies suggest that serotonin 5HT7 receptors associate with Gs�heterotrimers prior to agonist binding, and that 5HT7-Gs�complexes dissociate after the G protein is activated. Here we study this unconventional mode of coupling using bioluminescence resonance energy transfer (BRET) between luciferase-tagged 5HT7 receptors and Gs�heterotrimers labeled with Venus. Our results confirm that 5HT7 receptors interact with inactive (GDP-bound) Gs�heterotrimers in the absence of an agonist, and that this interaction is stabilized by the inverse agonist methiothepin. Stimulation with the endogenous agonist serotonin (5HT) decreased BRET between 5HT7 receptors and Gs, indicating that the activation of the receptor leads to 5HT7-Gscomplex dissociation. Interestingly, Gs�activation was not required for complex dissociation. These results are consistent with the hypothesis that 5HT7 receptors couple to Gs�heterotrimers via an unconventional mechanism involving ligand-sensitive complexes of receptors and inactive Gs.
    • Exact diagonalization RIXS studies of the doped 1d t1-t2-J model at the O K-edge

      Price, Gregory; Department of Chemistry & Physics; Datta, Trinanjan; Augusta University (2019-02-13)
      Resonant inelastic x-ray scattering (RIXS) is a novel spectroscopic method for probing charge and spin excitations in quantum magnets. In one dimension, where quantum fluctuations are most prominent, a system of interacting electrons can support fractionalized spinless charge excitations (holons) and chargeless spin excitation (spinons). Currently, X-ray spectroscopic techniques such as RIXS can excite the O K-edge core electrons of correlated quantum magnets to probe the physical nature of the above mentioned spin-charge separated state. Using exact diagonalization we investigate the O K-edge RIXS response of the one dimensional antiferromagnetic spin chain compound with nearest and next-nearest neighbor hoppings. We also study the spin-anisotropic version of the same model. Interaction of the core electrons with the X-rays generate multi-spinon excitations in the RIXS spectrum, for example in strontium copper oxide. We find that the RIXS spectrum of the t1-t2-J model with spin anisotropy presents a rich source of physical information, including allowing us to identify microscopic pathways for how the quantum spin fluctuations control the appearance of the four spinon excitations observed in the isotropic O K-edge spectrum.
    • Concentrations of Iron, Copper, Nickel, and Zinc in Rae's Creek

      Bridgers, Aerial; Department of Chemistry & Physics; Klug, Christopher; Augusta University (2019-02-13)
      Heavy metals can enter water systems through a variety of methods, such as through soil run-off, rain, or industrial activity near the system. Many of these heavy metals are toxic to both the wildlife and people around the system if present in high enough concentrations. The aim of this research was to create a model for a local water system, Rae's Creek, outlining the concentrations of iron, copper, nickel, and zinc present throughout a seven-month period. Additionally, this research sought to pinpoint any correlation between increases in metal concentrations and outside events, such as rain or the Master's Tournament held yearly in Augusta. Results indicated that copper and zinc concentrations were well above guidelines set for recreational water quality by the Environmental Protection Agency, while iron and nickel concentrations were generally below the limitations set for their concentrations. While rain had no observed effect on the heavy metal concentrations, there are two specific dates where all four metals had a marked increase in concentration. However, it is inconclusive as to what caused this increase.
    • FLATFISH ASSEMBLAGE AND ABUNDANCE DIFFERENCES BETWEEN THE SAINT CATHERINES ISLAND AND SATILLA RIVER ESTUARIES

      Bickle, Abigail; Coleman, Alex; Brown, Jason; Thiruvaiyaru, Dharma; Sethuraman, Sankar; Department of Biological Sciences; Department of Mathematics; Reichmuth, Jessica; Saul, Bruce; Augusta University (2019-02-13)
      St. Catherines Island is a pristine uninhabited barrier island off of the Georgia coast, and is believed to have experienced less anthropogenic alteration when compared to mainland estuarine habitats. In contrast, the Satilla River estuary has been historically affected by human activity, especially during the construction of navigational "cuts" through the marsh in the early 1900s. These cuts were used to support economic gain no longer in the area. Because of the differences in human influence, we hypothesize the fish assemblages will be different. We compared abundance of various resident flatfish species captured when trawling and using gill nets in these two systems. As benthic species, flatfish may be especially affected by anthropogenic disturbances of the estuarine substrates. This study provides insight into the effects of human disturbances on benthic fish species populations and assemblages. We compared catch-per-unit effort for six flatfish species between data among several sites, seasonally, between 2015 and 2018. We also looked at environmental variables when comparing abundance. The data are reflective of differences that exist in resident flatfish populations, and this condition could be explained by anthropogenic activities.
    • WILLIAMSON ETHER REACTION USING A SOLAR HEAT SOURCE DESIGNED FOR UNDERGRADUATE CHEMISTRY LABORATORIES

      Hammond, Caroline; Wyman, Kailey; Blair, Gregory; Department of Chemistry and Physics; Agee, Brian; Augusta University (2019-02-13)
      Since the 1990�s, scientists have been attempting to make chemical synthesis procedures more environmentally friendly.� One area of environmental concern is the amount of electricity required to provide enough energy to complete an experiment. Recently proposed solar reflectors developed from satellite dishes have the ability to be incorporated into student laboratory procedures to eliminate electricity use while demonstrating green chemistry techniques at the same time. As a result, demand to incorporate more green chemistry techniques into student laboratories has increased. An effective means for minimizing the amount of electricity needed to drive chemical reactions to completion is proposed through the use of solar parabolic reflectors. A comparative study was conducted using an electrical and solar heat source on the Williamson Ether synthesis of 2-butoxynaphthalene. This reaction was chosen as the test reaction due to its widespread use among many undergraduate chemistry programs.
    • INVESTIGATING THE REQUIREMENT OF HOB1 ON THE SENSITIVITY OF�SCHIZOSACCHAROMYCES POMBE�AFTER EXPOSURE TO VARIOUS DNA DAMAGING AGENTS

      Qureshi, Arman; Department of Biological Sciences; Abdulovic-Cui, Amy L; Augusta University (2019-02-13)
      It is inherently important that when damaged, DNA is repaired efficiently and with high accuracy. BIN1 encodes a protein that plays a role in genomic stability, specifically in cell cycle regulation, chromatin remodeling, and DNA repair. Previous research has shown that the protein Bin1 exhibits an inhibitory role in the double strand break repair pathway of non-homologous end joining (NHEJ). The homolog of�BIN1,�HOB1, is found in the fission yeast,�Schizosaccharomyces pombe. To understand the role�HOB1�has on yeast survival after damage, two strains of�S. pombe, a wild type strain (WT) and a strain without�HOB1�(hob1?), were exposed to various DNA damaging agents. Each treatment introduced different types of DNA damage that require repair by different DNA repair pathways. These treatments included UV radiation, hydrogen peroxide treatment, Bleomycin treatment, and Cisplatin Treatment. After treatment with each respective agent, the death response of each strain was calculated and the % of surviving cells at multiple doses was graphed logarithmically. The data collected overwhelming support the idea that the presence of�HOB1�has a positive role on the survival of yeast after DNA damage. The WT strains tested survived better than the�hob1?�counterparts.
    • Racial Dissimilarities as a Social Determinant of Health Outcomes: Evidence from Counties in the State of Georgia

      Lee, Divesia; Hull College of Business; Department of English & Foreign Languages; Medcalfe, Simon; Slade, Catherine; Hoffman, Todd; Augusta University (2019-02-13)
      Social determinants of health account for about 50 percent of health outcomes- more than any other category, yet is the most understudied, therefore warranting further investigation. We contend that within social determinants of health, analysis of racial segregation is of importance. Racial segregation is a structural form of racism, where people of similar race live in communities apart from people of other races. Prior studies have used a dissimilarity index to measure racial segregation and its impact on health outcomes, and has suggested that racial residential segregation has a negative impact on health outcomes, but none of these studies have focused on county level data or the State of Georgia in particular. Using a dataset from the Robert Wood Johnson Foundation, supplemented by other public health and demographic data for all counties in Georgia, we use regression analysis to model the relationship between segregation and various health outcomes. A variety of social determinants of health were analyzed ranging from factors of economic stability, neighborhood and physical environment, and education, to aspects of the healthcare system. Initial results suggest that racial segregation relates to health outcomes, but it depends on the health outcomes being measured. Conclusions are pending further quantitative analysis.
    • Coffee In Augusta

      Guajardo, Aleighna; Department of Communication; Department of Anthropology & Philosophy; Bratton, Angela; Bryant, Will; Augusta University (2019-02-13)
      For my Honors thesis, I produced a short documentary on local coffee shops in Augusta, focusing on local coffee shops that offer unique environments for their customers. After viewing several documentaries on coffee for reference, I filmed and edited my finished product to demonstrate the importance of supporting local coffee shops, the hard work that goes into opening up a shop in the coffee business, and bring awareness to a few of the different shops currently operating in Augusta. The documentary briefly covers the history of each cafe and incorporates footage of normal business taken from within the coffee shops; such as, cashiers taking orders and baristas making a variety of coffee drinks. The film communicates through interviews, b roll, and music the true ambience of the coffee shops and what qualities these cafes offer to customers that is distinct from larger franchises. Each coffee shop included in the documentary offers a unique environment that is key to what is referred to as the third space of society, a mainly social location that is neither home nor work. This concept of third space from anthropology is defined and explored throughout the film to show the importance of small local coffee shops.
    • Blurred Lines within the Music Industry: A Different Perspective of Copyright Law and Sampling in the Digital Age

      Wingate, Montrel; Department of History, Anthropology & Philosophy; Turner, Wendy; VanTuyll, Debra; VanTuyll, Hubert; Augusta University (2019-02-13)
      This thesis focuses on the relationship of music and law. Throughout, the debated question is: should the laws of copyright be redefined? The case Williams v. Bridgeport Music, Inc., which focuses on the songs "Blurred Lines" by Robin Thicke and "Got to Give It Up" by Marvin Gaye is the trial central to this thesis. Following a brief history of sampling, Williams v. Bridgeport Music, Inc. is reexamined, challenging the substantiality of the evidence presented. The court proved that the songs have similarities on the surface, yet there is a notable structural difference among the songs. A proposed solution is given, advocating a revision of copyright laws and a substantive similarity test with emphasis on the expert listener rather than the lay listener.
    • Transport properties in Graphene Bilayer

      Trowel, Alonte; Department of Chemistry & Physics; Datta, Trinanjan; Augusta University (2019-02-13)
      Graphene is a single layer of carbon atoms arranged in a hexagonal pattern. It has many potential technological applications and provides a testbed to verify fundamental concepts in physics. Using quantum mechanical transmission and reflection amplitudes we study the transport properties of bilayer graphene. For the parameter range that We explored we find that the transmission probability is controlled by the applied bias. We also outline how this approach can be utilized to study oligomers and oligoacenes.